The DOI matches its Crossref record; 18 of the 18 references it cites were found in a registry or an index.
Constitutive expression of the phoP regulon attenuates Salmonella virulence and survival within macrophages
Open accessAbstract
The phoP genetic locus is a two-component regulatory system (phoP-phoQ) that controls the expression of genes essential for Salmonella typhimurium virulence and survival within macrophages. Strains with a phoP constitutive mutation (phenotype PhoPC) showed up to 10-fold greater expression of phoP-activated genes (pag loci) than did strains with a wild-type phoP locus (phenotype PhoP+). While the phoP constitutive mutation resulted in increased expression of pag loci, it also dramatically reduced the expression of other protein species. Comparison of the protein content of PhoP+ and PhoPC strains by two-dimensional protein gel electrophoresis demonstrated that at least 40 separate protein species were changed in expression as a result of this mutation. The PhoPC S. typhimurium were found to be attenuated for virulence and survival within macrophages. This finding suggests that a balanced PhoP-PhoQ regulatory response, which allows expression of phoP-repressed as well as -activated genes, is required for full virulence of S. typhimurium. We have further shown that small numbers of PhoPC bacteria can be used as a live attenuated vaccine to protect against mouse typhoid. As few as 15 PhoPC bacteria protected mice against challenge with 10(5) 50% lethal doses of wild-type organisms, suggesting that important protective antigens are regulated by the PhoP-PhoQ virulence regulon.
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Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
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O'Farrell, P. (1975) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)41496-8 · Cited by 19,246
Mutants of Salmonella typhimurium that cannot survive within the macrophage are avirulent.
Fields, P. I., Swanson, R. V., Haidaris, C. G., & Heffron, F. (1986) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.83.14.5189 · Cited by 1,114
Taylor, R. K., Miller, V. L., Furlong, D. B., & Mekalanos, J. J. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.9.2833 · Cited by 1,054
Self-citationA two-component regulatory system (phoP phoQ) controls Salmonella typhimurium virulence.
Miller, S. I., Kukral, A. M., & Mekalanos, J. J. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.13.5054 · Cited by 929
Self-citationRegulation of exoprotein gene expression in Staphylococcus aureus by agr
Recsei, P., Kreiswirth, B., O'Reilly, M., Schlievert, P., Gruss, A., & Novick, R. P. (1986) · Molecular and General Genetics MGG · doi:10.1007/bf00330517 · Cited by 618
Coordinate Regulation and Sensory Transduction in the Control of Bacterial Virulence
Miller, J. F., Mekalanos, J. J., & Falkow, S. (1989) · Science · doi:10.1126/science.2537530 · Cited by 616
Self-citationA Salmonella Locus that Controls Resistance to Microbicidal Proteins from Phagocytic Cells
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Cited by
COMMUNICATION MODULES IN BACTERIAL SIGNALING PROTEINS
Parkinson, J. S., & Kofoid, E. C. (1992) · Annual Review of Genetics · doi:10.1146/annurev.ge.26.120192.000443 · Cited by 1,450
The Pleiotropic Two-Component Regulatory System PhoP-PhoQ
Groisman, E. A. (2001) · Journal of Bacteriology · doi:10.1128/jb.183.6.1835-1842.2001 · Cited by 887
Salmonellae interplay with host cells
Haraga, A., Ohlson, M. B., & Miller, S. I. (2008) · Nature Reviews Microbiology · doi:10.1038/nrmicro1788 · Cited by 832
Self-citationEnvironmental signals controlling expression of virulence determinants in bacteria
Mekalanos, J. J. (1992) · Journal of Bacteriology · doi:10.1128/jb.174.1.1-7.1992 · Cited by 788
Self-citationGunn, J. S., Lim, K. B., Krueger, J., Kim, K., Guo, L., Hackett, M., & Miller, S. I. (1998) · Molecular Microbiology · doi:10.1046/j.1365-2958.1998.00757.x · Cited by 655
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Metadata
- Authors
- Miller, S. I., & Mekalanos, J. J.
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 1990-05
- Volume
- 172
- Issue
- 5
- Pages
- 2485-2490
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- OpenAlex
- W1897079930
- Cited by
- 395
- References
- 18
- FWCI
- 8.29
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Miller, S. I., & Mekalanos, J. J. (1990). Constitutive expression of the phoP regulon attenuates Salmonella virulence and survival within macrophages. Journal of Bacteriology. https://doi.org/10.1128/jb.172.5.2485-2490.1990
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
