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Cited by 83Bacterial Genetics and BiotechnologyFungal and yeast genetics researchBiochemical and Structural Characterization

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The primary role of comA in establishment of the competent state in Bacillus subtilis is to activate expression of srfA

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Nakano, M M, Zuber, P·1991·Journal of Bacteriology
DOI 10.1128/jb.173.22.7269-7274.1991PMC PMC209234
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Abstract

The establishment of genetic competence in Bacillus subtilis requires the genes of the competence regulon which function in the binding, processing, and transport of DNA. Their expression is governed by multiple regulatory pathways that are composed of the comA, comP, sin, abrB, spo0H, spo0K, spo0A, degU, and srfA gene products. Among these, srfA is thought to occupy an intermediate position in one of the pathways that controls late competence gene expression. The full expression of srfA requires the gene products of comP, comA, and spo0K. To determine the role of these genes in the regulation of competence development, the expression of the srfA operon was placed under control of the isopropyl-beta-D-thiogalactopyranoside (IPTG)-inducible promoter Pspac and the expression of the Pspac-srfA construct was examined in mutants blocked in early competence. By monitoring the IPTG-induced expression of Pspac-srfA with a srfA-lacZ operon fusion, it was observed that srfA expression was no longer dependent on the products of comP, comA, and spo0K. Production of the lipopeptide antibiotic surfactin in Pspac-srfA-bearing cells was induced in the presence of IPTG and was independent of ComP and ComA. Competence development was induced by IPTG and was independent of comP, comA, and spo0K in cells carrying Pspac-srfA. These results suggest that the ComP-ComA signal transduction pathway as well as Spo0K is required for the expression of srfA in the regulatory cascade of competence development. Studies of Pspac-srfA also examined the involvement of srfA in the growth stage-specific and nutritional regulation of a late competence gene.

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Cited by

Metadata

Authors
Nakano, M. M., & Zuber, P.
Affiliations
Louisiana State University (US)
ORCID
Venue
Journal of Bacteriology
Publisher
American Society for Microbiology
Published
1991-11
Volume
173
Issue
22
Pages
7269-7274
ISSN
  • 0021-9193 (ISSN-L)
  • 1098-5530
DOI
10.1128/jb.173.22.7269-7274.1991
PMC
PMC209234
OpenAlex
W2131154089
Cited by
83
References
0
Citations by year
2026: 1 · 2024: 2 · 2023: 2 · 2022: 1 · 2021: 2 · 2020: 2 · 2018: 4 · 2017: 1 · 2016: 3 · 2015: 1 · 2014: 10
FWCI
3.64
Type
journal article
Language
en
Open access
Licence
journals.asm.org/non-commercial-tdm-license (tdm)
Locations
  • Journal of Bacteriology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health
  • National Institute of General Medical Sciences (GM39476)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
Keywords
Bacillus subtilis, genetic competence, coma, COMP, competence regulon, surfactin, signal transduction, Spo0A, nutritional regulation
MeSH terms
Bacillus subtilis, beta-Galactosidase, Chromosome Mapping, Chromosomes, Bacterial, Genes, Bacterial, Genes, Regulator, Genotype, Phenotype, Plasmids, Promoter Regions, Genetic, Transformation, Bacterial, Restriction Mapping, Gene Expression Regulation, Bacterial

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