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Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system

Bikard, David, Jiang, Wenyan, Samai, Poulami, Hochschild, Ann, Zhang, Feng, Marraffini, Luciano A.·2013·Nucleic Acids Research
DOI 10.1093/nar/gkt520PMC PMC3753641
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  • Due to its catalytically inactive nature, modifications of gene expression caused by dCas9 are transient, since the genomic DNA is not altered.

    “Due to its catalytically inactive nature, modifications of gene expression caused by dCas9 are transient, since the genomic DNA is not altered [61,77].”

    From CRISPR-Cas: Converting A Bacterial Defence Mechanism into A State-of-the-Art Genetic Manipulation Tool (2019) · cites this paper as [61]

    The Introduction says dCas9’s mutations abolish DNA cleavage without impairing DNA binding, and the Results attribute repression to that binding rather than DNA editing. The Discussion reports that repression can be reversed when an inducer is withdrawn, but it does not establish that dCas9-mediated expression changes are always transient; reversibility there depends on withdrawing the inducer. Page numbers were not available in our copy. (LLM, conf=0.84)

    Experimental — accuracy not yet measured

  • By introducing two mutations in the RuvC and HNH catalytic domains of Cas9, scientists engineered a 'dead' Cas9 (dCas9) that could still bind to DNA but had no cleaving activity.

    “By introducing two mutations in the RuvC and HNH catalytic domains of Cas9, scientists engineered a ‘dead’ Cas9 (dCas9) that could still bind to DNA but had no cleaving activity [61].”

    From CRISPR-Cas: Converting A Bacterial Defence Mechanism into A State-of-the-Art Genetic Manipulation Tool (2019) · cites this paper as [61]

  • Bikard et al. demonstrated that CRISPR could be used as a new tool to regulate gene expression by either activating or repressing the transcription of bacterial genes.

    “Later in the same year, Bikard et al. demonstrated how CRISPR could be used as a new tool to regulate gene expression by either activating or repressing the transcription of bacterial genes [61].”

    From CRISPR-Cas: Converting A Bacterial Defence Mechanism into A State-of-the-Art Genetic Manipulation Tool (2019) · cites this paper as [61]

  • When both RuvC and HNH catalytic domains of Cas9 are modified through two silencing mutations, the system loses DNA cleaving capabilities but retains ability to bind targeted sequences.

    “When both RuvC and HNH catalytic domains of Cas9 are modified through two silencing mutations, the system loses its DNA cleaving capabilities but retains the ability to bind to targeted sequences [61,77].”

    From CRISPR-Cas: Converting A Bacterial Defence Mechanism into A State-of-the-Art Genetic Manipulation Tool (2019) · cites this paper as [61]

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Research works printed but not linked: 36

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Metadata

Authors
Bikard, D., Jiang, W., Samai, P., Hochschild, A., Zhang, F., & Marraffini, L. A.
Venue
Nucleic Acids Research
Publisher
Oxford University Press (OUP)
Published
2013-06-12
Volume
41
Issue
15
Pages
7429-7437
ISSN
  • 1362-4962
  • 0305-1048
DOI
10.1093/nar/gkt520
PMC
PMC3753641
Cited by
1,145
References
9
Language
en
Licence
creativecommons.org/licenses/by-nc/3.0/ (vor, 1244 d)

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