The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.
Cryo-Transmission Electron Microscopy of Frozen-Hydrated Sections of Escherichia coli and Pseudomonas aeruginosa
Open accessAbstract
High-pressure freezing of Escherichia coli K-12 and Pseudomonas aeruginosa PAO1 in the presence of cryoprotectants provided consistent vitrification of cells so that frozen-hydrated sections could be cut, providing ∼2-nm resolution of structure. The size and shape of the bacteria, as well as their surface and cytoplasmic constituents, were nicely preserved and compared well with other published high-resolution techniques. Cells possessed a rich cytoplasm containing a diffuse dispersion of ribosomes and genetic material. Close examination of cells revealed that the periplasmic space was compressed during cryosectioning, a finding which provided supporting evidence that this space is filled by a compressible gel. Since the outer membrane and peptidoglycan layer are bonded together via lipoproteins, the space between them (although still part of the periplasmic space) was not as compacted. Even when this cryosectioning compression was taken into account, there was still substantial variability in the width of the periplasmic space. It is possible that the protoplast has some capacity to float freely within the periplasm.
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Cites
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Cryo-electron microscopy of vitrified specimens
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Self-citationStructures of Gram-Negative Cell Walls and Their Derived Membrane Vesicles
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Self-citationFINE STRUCTURE IN FROZEN-ETCHED YEAST CELLS
Moor, H., & Mühlethaler, K. (1963) · The Journal of Cell Biology · doi:10.1083/jcb.17.3.609 · Cited by 1,332
Practical Methods in Electron Microscopy
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Crystal structure of bacterial multidrug efflux transporter AcrB
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Cited by
Peptidoglycan structure and architecture
Vollmer, W., Blanot, D., & De Pedro, M. A. (2008) · FEMS Microbiology Reviews · doi:10.1111/j.1574-6976.2007.00094.x · Cited by 2,340
Biosynthesis and Assembly of Capsular Polysaccharides in Escherichia coli
Whitfield, C. (2006) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.75.103004.142545 · Cited by 990
Murata, H., Koepsel, R. R., Matyjaszewski, K., & Russell, A. J. (2007) · Biomaterials · doi:10.1016/j.biomaterials.2007.06.012 · Cited by 722
Biosynthesis and Export of Bacterial Lipopolysaccharides
Whitfield, C., & Trent, M. S. (2014) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-060713-035600 · Cited by 709
Hoffmann, C., Leis, A., Niederweis, M., Plitzko, J. M., & Engelhardt, H. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0709530105 · Cited by 596
Metadata
- Authors
- Matias, V. R. F., Al-Amoudi, A., Dubochet, J., & Beveridge, T. J.
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 2003-10-15
- Volume
- 185
- Issue
- 20
- Pages
- 6112-6118
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- OpenAlex
- W1982889726
- Cited by
- 388
- References
- 36
- FWCI
- 11.32
- Type
- journal article
- Language
- en
- Open access
- green
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- Matias, V. R. F., Al-Amoudi, A., Dubochet, J., & Beveridge, T. J. (2003). Cryo-Transmission Electron Microscopy of Frozen-Hydrated Sections of Escherichia coli and Pseudomonas aeruginosa. Journal of Bacteriology. https://doi.org/10.1128/jb.185.20.6112-6118.2003
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
