The DOI matches its Crossref record; 46 of the 46 references it cites were found in a registry or an index.
The PmrA-Regulated pmrC Gene Mediates Phosphoethanolamine Modification of Lipid A and Polymyxin Resistance in Salmonella enterica
Open accessAbstract
The PmrA/PmrB regulatory system of Salmonella enterica controls the modification of lipid A with aminoarabinose and phosphoethanolamine. The aminoarabinose modification is required for resistance to the antibiotic polymyxin B, as mutations of the PmrA-activated pbg operon or ugd gene result in strains that lack aminoarabinose in their lipid A molecules and are more susceptible to polymyxin B. Additional PmrA-regulated genes appear to participate in polymyxin B resistance, as pbgP and ugd mutants are not as sensitive to polymyxin B as a pmrA mutant. Moreover, the role that the phosphoethanolamine modification of lipid A plays in the resistance to polymyxin B has remained unknown. Here we address both of these questions by establishing that the PmrA-activated pmrC gene encodes an inner membrane protein that is required for the incorporation of phosphoethanolamine into lipid A and for polymyxin B resistance. The PmrC protein consists of an N-terminal region with five transmembrane domains followed by a large periplasmic region harboring the putative enzymatic domain. A pbgP pmrC double mutant resembled a pmrA mutant both in its lipid A profile and in its susceptibility to polymyxin B, indicating that the PmrA-dependent modification of lipid A with aminoarabinose and phosphoethanolamine is responsible for PmrA-regulated polymyxin B resistance.
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Metadata
- Authors
- Lee, H., Hsu, F.-F., Turk, J., & Groisman, E. A.
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 2004-07
- Volume
- 186
- Issue
- 13
- Pages
- 4124-4133
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- OpenAlex
- W2115996594
- Cited by
- 334
- References
- 46
- FWCI
- 8.25
- Type
- journal article
- Language
- en
- Open access
- green
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- Lee, H., Hsu, F.-F., Turk, J., & Groisman, E. A. (2004). The PmrA-Regulated pmrC Gene Mediates Phosphoethanolamine Modification of Lipid A and Polymyxin Resistance in Salmonella enterica. Journal of Bacteriology. https://doi.org/10.1128/jb.186.13.4124-4133.2004
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