The DOI matches its Crossref record; 37 of the 37 references it cites were found in a registry or an index.
High- and Low-Threshold Genes in the Spo0A Regulon of Bacillus subtilis
Open accessAbstract
The master regulator for entry into sporulation in Bacillus subtilis is the response regulator Spo0A, which directly governs the expression of about 121 genes. Using cells in which the synthesis of Spo0A was under the control of an inducible promoter or in which production of the regulatory protein was impaired by a promoter mutation, we found that sporulation required a high (threshold) level of Spo0A and that many genes in the regulon differentially responded to high and low doses of the regulator. We distinguished four categories of genes, as follows: (i) those that required a high level of Spo0A to be activated, (ii) those that required a high level of Spo0A to be repressed, (iii) those that were activated at a low level of the regulator, and (iv) those that were repressed at a low dose of the regulator. Genes that required a high dose of Spo0A to be activated were found to have low binding constants for the DNA-binding protein. Some genes that were turned on at a low dose of Spo0A either had a high binding constant for the regulatory protein or were activated by an indirect mechanism involving Spo0A-mediated relief of repression by the repressor protein AbrB. We propose that progressive increases in the level of Spo0A leads to an early phase of transcription in which genes that play auxiliary roles in development, such as cannibalism and biofilm formation, are turned on and a later phase in which genes that play a direct role in sporulation are activated.
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Cites
Fruiting body formation by Bacillus subtilis
Branda, S. S., González-Pastor, J. E., Ben-Yehuda, S., Losick, R., & Kolter, R. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.191384198 · Cited by 1,129
Self-citationSonenshein, A. L., Hoch, J. A., & Losick, R. M. (1993) · Cited by 1,083
Self-citationInitiation of sporulation in B. subtilis is controlled by a multicomponent phosphorelay
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Bacillus Subtilis and Its Closest Relatives: From Genes to Cells
Sonenshein, A. L., Hoch, J. A., & Losick, R. M. (2001) · Cited by 674
Self-citationA master regulator for biofilm formation by Bacillus subtilis
Kearns, D. B., Chu, F., Branda, S. S., Kolter, R., & Losick, R. (2005) · Molecular Microbiology · doi:10.1111/j.1365-2958.2004.04440.x · Cited by 597
Self-citationCannibalism by sporulating bacteria.
González-Pastor, J. E., Hobbs, E. C., & Losick, R. (2003) · Science · doi:10.1126/science.1086462 · Cited by 579
Self-citationThe Spo0A regulon of Bacillus subtilis
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Self-citationPlasmids for ectopic integration in Bacillus subtilis
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The sporulation transcription factor Spo0A is required for biofilm development in Bacillus subtilis
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Cited by
Signals, Regulatory Networks, and Materials That Build and Break Bacterial Biofilms
Karatan, E., & Watnick, P. (2009) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00041-08 · Cited by 1,048
Sticking together: building a biofilm the Bacillus subtilis way
Vlamakis, H., Chai, Y., Beauregard, P., Losick, R., & Kolter, R. (2013) · Nature Reviews Microbiology · doi:10.1038/nrmicro2960 · Cited by 1,036
Self-citationBistability, Epigenetics, and Bet-Hedging in Bacteria
Veening, J.-W., Smits, W. K., & Kuipers, O. P. (2008) · Annual Review of Microbiology · doi:10.1146/annurev.micro.62.081307.163002 · Cited by 1,018
Signal integration in bacterial two-component regulatory systems
Mitrophanov, A. Y., & Groisman, E. A. (2008) · Genes & Development · doi:10.1101/gad.1700308 · Cited by 414
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Metadata
- Authors
- Fujita, M., González-Pastor, J. E., & Losick, R.
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 2005-02-15
- Volume
- 187
- Issue
- 4
- Pages
- 1357-1368
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- OpenAlex
- W2052205534
- Cited by
- 427
- References
- 37
- FWCI
- 9.75
- Type
- journal article
- Language
- en
- Open access
- green
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- Fujita, M., González-Pastor, J. E., & Losick, R. (2005). High- and Low-Threshold Genes in the Spo0A Regulon of Bacillus subtilis. Journal of Bacteriology. https://doi.org/10.1128/jb.187.4.1357-1368.2005
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