The DOI matches its Crossref record; 53 of the 53 references it cites were found in a registry or an index.
The sporulation transcription factor Spo0A is required for biofilm development in Bacillus subtilis
Abstract
Biofilms are structured communities of cells encased in a polymeric matrix and adherent to a surface, interface or each other. We report here that the soil bacterium Bacillus subtilis forms biofilms. By confocal scanning laser microscopy, we observed that B. subtilis adhered to abiotic surfaces and formed a three‐dimensional structure ≥ 30 µm in depth. These biofilms appeared to be at least partly encased in an extracellular polysaccharide matrix, as they could be stained with Calcofluor, a polysaccharide‐binding dye. To understand the molecular mechanism of biofilm formation, we screened previously characterized mutants for a defect in biofilm formation. We found that mutations in spo0A, which encodes the major early sporulation transcription factor, caused a defect in biofilm formation. spo0A mutant cells adhered to a surface in a monolayer of cells rather than a three‐dimensional biofilm. The requirement of Spo0A for biofilm development appears to result from its role in negatively regulating AbrB. Mutations in abrB suppressed the biofilm defect of a spo0A mutant, indicating that AbrB negatively regulates at least one gene that is required for the transition from a monolayer of attached cells to a mature biofilm. Implications of biofilm development for the ecology of B. subtilis are discussed.
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Biofilm formation as microbial development
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The Involvement of Cell-to-Cell Signals in the Development of a Bacterial Biofilm
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Microbial Biofilms: from Ecology to Molecular Genetics
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Cited by
Sporulation of Bacillus subtilis
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“At the same time it was noted that laboratory isolates of B. subtilis had lost the capability to form rugose biofilms (Branda et al. 2001) but retained the capacity to develop submerged surface-adhered biofilms (Hamon and Lazazzera 2001).”
BackgroundGenomics of the group of organisms
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Beneficial biofilm formation by industrial bacteria Bacillus subtilis and related species
Morikawa, M. (2006) · Journal of Bioscience and Bioengineering · doi:10.1263/jbb.101.1 · Cited by 265
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Metadata
- Authors
- Hamon, M. A., & Lazazzera, B. A.
- Venue
- Molecular Microbiology
- Publisher
- Wiley
- Published
- 2001-12
- Volume
- 42
- Issue
- 5
- Pages
- 1199-1209
- ISSN
- 0950-382X (ISSN-L)
- 1365-2958
- OpenAlex
- W1826314932
- Cited by
- 444
- References
- 53
- FWCI
- 2.93
- Type
- journal article
- Language
- en
- Open access
- closed
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- Hamon, M. A., & Lazazzera, B. A. (2001). The sporulation transcription factor Spo0A is required for biofilm development in Bacillus subtilis. Molecular Microbiology. https://doi.org/10.1046/j.1365-2958.2001.02709.x
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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