The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
The Intestinal Life Cycle of Bacillus subtilis and Close Relatives
Open accessAbstract
Bacillus subtilis is considered a soil organism for which endospore formation provides a means to ensure long-term survival in the environment. We have addressed here the question of what happens to a spore when ingested. Spores displaying on their surface a heterologous antigen, tetanus toxin fragment C (TTFC), were shown to generate anti-TTFC responses not to the antigen contained in the primary oral inoculum but to those displayed on spores that had germinated and then resporulated. We then used reverse transcription-PCR to determine expression of vegetative genes and sporulation-specific genes in the mouse gut following oral dosing with spores. Significant levels of germination and sporulation were documented. Using natural isolates of B. subtilis that could form biofilms, we showed that these strains could persist in the mouse gut for significantly longer than the laboratory strain. Moreover, these isolates could grow and sporulate anaerobically and exhibited a novel phenomenon of being able to form spores in almost half the time required for the laboratory isolate. This suggests that spores are not transient passengers of the gastrointestinal tract but have adapted to carry out their entire life cycle within this environment. This is the first report showing an intestinal life cycle of B. subtilis and suggests that other Bacillus species could also be members of the gut microflora.
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Cites
Molecular biological methods for Bacillus
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Self-citationFruiting body formation by Bacillus subtilis
Branda, S. S., González-Pastor, J. E., Ben-Yehuda, S., Losick, R., & Kolter, R. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.191384198 · Cited by 1,129
The use of bacterial spore formers as probiotics: Table 1
Hong, H. A., Duc, L. H., & Cutting, S. M. (2005) · FEMS Microbiology Reviews · doi:10.1016/j.femsre.2004.12.001 · Cited by 975
Self-citationMock, M., & Fouet, A. (2001) · Annual Review of Microbiology · doi:10.1146/annurev.micro.55.1.647 · Cited by 894
Sporulation germination and outgrowth
Nicholson, W. L. (1990) · Medical Entomology and Zoology · Cited by 858
Bacillus subtilis sporulation: regulation of gene expression and control of morphogenesis
Errington, J. (1993) · Microbiological Reviews · doi:10.1128/mmbr.57.1.1-33.1993 · Cited by 644
Plasmids for ectopic integration in Bacillus subtilis
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The hidden lifestyles of Bacillus cereus and relatives
Jensen, G. B., Hansen, B. M., Eilenberg, J., & Mahillon, J. (2003) · Environmental Microbiology · doi:10.1046/j.1462-2920.2003.00461.x · Cited by 475
Roles of Bacillus endospores in the environment
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Characterization of Bacillus Probiotics Available for Human Use
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Bacillus As Potential Probiotics: Status, Concerns, and Future Perspectives
Elshaghabee, F. M. F., Rokana, N., Gulhane, R. D., Sharma, C., & Panwar, H. (2017) · Frontiers in Microbiology · doi:10.3389/fmicb.2017.01490 · Cited by 940
Cutting, S. M. (2011) · Food Microbiology · doi:10.1016/j.fm.2010.03.007 · Cited by 787
Self-citationHobley, L., Harkins, C., MacPhee, C. E., & Stanley-Wall, N. R. (2015) · FEMS Microbiology Reviews · doi:10.1093/femsre/fuv015 · Cited by 581
“…ability of some B. subtilis isolates to persist within the gastrointestinal tract of mice, and whilst a definitive cause and effect has not yet been shown it has been hypothesized that biofilm formation may be responsible for the persistence of germinated spores after ingestion (Tam et al. 2006).”
BackgroundEcology and genomics of Bacillus subtilis
Earl, A. M., Losick, R., & Kolter, R. (2008) · Trends in Microbiology · doi:10.1016/j.tim.2008.03.004 · Cited by 560
Genomic reconstruction of the transcriptional regulatory network in Bacillus subtilis.
Leyn, S. A., Kazanov, M. D., Sernova, N. V., Ermakova, E. O., Novichkov, P. S., & Rodionov, D. A. (2013) · Journal of Bacteriology · doi:10.1128/jb.00140-13 · Cited by 58
Metadata
- Authors
- Tam, N. K. M., Uyen, N. Q., Hong, H. A., Duc, L. H., Hoa, T. T., Serra, C. R., Henriques, A. O., & Cutting, S. M.
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 2006-04
- Volume
- 188
- Issue
- 7
- Pages
- 2692-2700
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- OpenAlex
- W2112489212
- Cited by
- 334
- References
- 25
- FWCI
- 4.91
- Type
- journal article
- Language
- en
- Open access
- green
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- Tam, N. K. M., Uyen, N. Q., Hong, H. A., Duc, L. H., Hoa, T. T., Serra, C. R., Henriques, A. O., & Cutting, S. M. (2006). The Intestinal Life Cycle of Bacillus subtilis and Close Relatives. Journal of Bacteriology. https://doi.org/10.1128/jb.188.7.2692-2700.2006
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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