The DOI matches its Crossref record, with a small difference in title or year; 20 of the 20 references it cites were found in a registry or an index.
Development of a Singleplex PCR Assay for Rapid Identification and Differentiation of Cryptococcus neoformans var.grubii, Cryptococcus neoformans var. neoformans, Cryptococcus gattii, and Hybrids
Open accessAbstract
A singleplex PCR assay using a single primer pair targeting the putative sugar transporter gene was developed here to distinguish Cryptococcus neoformans var.grubii,Cryptococcus neoformans var.neoformans, and Cryptococcus gattii according to the distinct size of the amplicon. The interspecies and intravarietal hybrids were also characterized on the basis of distinct combined profiles of amplicons. This PCR assay is a rapid, simple, and reliable approach suitable for laboratory diagnoses and large-scale epidemiologic studies.
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“More recently, molecular methods for detection of both C. neoformans and C. gattii have been reported that may improve laboratory diagnosis (26, 27).”
From Multicenter Evaluation of BioFire FilmArray Meningitis/Encephalitis Panel for Detection of Bacteria, Viruses, and Yeast in Cerebrospinal Fluid Specimens (2016) · cites this paper as [27]
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Cites
Schoch, C. L., Seifert, K. A., Huhndorf, S., Robert, V., Spouge, J. L., Levesque, C. A., Chen, W., Fungal Barcoding Consortium, Fungal Barcoding Consortium Author List, Bolchacova, E., et al. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1117018109 · Cited by 5,120
LAGAN and Multi-LAGAN: Efficient tools for large-scale multiple alignment of genomic DNA
Brudno, M., Do, C. B., Cooper, G. M., Kim, M. F., Davydov, E., Program, N. C. S., Green, E. D., Sidow, A., & Batzoglou, S. (2003) · Genome Research · doi:10.1101/gr.926603 · Cited by 1,115
Molecular Typing of IberoAmerican Cryptococcus neoformans Isolates
Meyer, W., Castañeda, A., Jackson, S., Huynh, M., Castañeda, E., & the IberoAmerican Cryptococcal Study Group (2003) · Emerging Infectious Diseases · doi:10.3201/eid0902.020246 · Cited by 520
Clinical and Host Differences Between Infections with the Two Varieties of Cryptococcus neoformans
Speed, B., & Dunt, D. (1995) · Clinical Infectious Diseases · doi:10.1093/clinids/21.1.28 · Cited by 478
MacDougall, L., Kidd, S. E., Galanis, E., Mak, S., Leslie, M. J., Cieslak, P. R., Kronstad, J. W., Morshed, M. G., & Bartlett, K. H. (2007) · Emerging Infectious Diseases · doi:10.3201/eid1301.060827 · Cited by 285
Litvintseva, A. P., Thakur, R., Vilgalys, R., & Mitchell, T. G. (2006) · Genetics · doi:10.1534/genetics.105.046672 · Cited by 265
αADα Hybrids of Cryptococcus neoformans: Evidence of Same-Sex Mating in Nature and Hybrid Fitness
Lin, X., Litvintseva, A. P., Nielsen, K., Patel, S., Floyd, A. M., Mitchell, T. G., & Heitman, J. (2007) · PLoS Genetics · doi:10.1371/journal.pgen.0030186 · Cited by 136
Tamura, M., Watanabe, K., Mikami, Y., Yazawa, K., & Nishimura, K. (2001) · Journal of Clinical Microbiology · doi:10.1128/jcm.39.12.4309-4315.2001 · Cited by 116
McTaggart, L. R., Lei, E., Richardson, S. E., Hoang, L., Fothergill, A. W., & Zhang, S. X. (2011) · Journal of Clinical Microbiology · doi:10.1128/jcm.00651-11 · Cited by 106
AIDS Patient Death Caused by NovelCryptococcus neoformans×C.gattiiHybrid
Bovers, M., Hagen, F., Kuramae, E. E., Hoogveld, H. L., Dromer, F., St‐Germain, G., & Boekhout, T. (2008) · Emerging infectious diseases · doi:10.3201/eid1407.080122 · Cited by 98
Cited by
Leber, A. L., Everhart, K., Balada-Llasat, J.-M., Cullison, J., Daly, J., Holt, S., Lephart, P., Salimnia, H., Schreckenberger, P. C., DesJarlais, S., et al. (2016) · Journal of Clinical Microbiology · doi:10.1128/jcm.00730-16 · Cited by 609
(2014) · doi:10.1128/cmr.00126-13 · Cited by 437
Metadata
- Authors
- Feng, X., Fu, X., Ling, B., Wang, L., Liao, W., & Yao, Z.
- Affiliations
- Shanghai Jiao Tong University (CN)
- Tongji University (CN)
- Second Military Medical University (CN)
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2013-06
- Volume
- 51
- Issue
- 6
- Pages
- 1920-1923
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- PMC
- PMC3716086
- OpenAlex
- W2157319636
- Cited by
- 38
- References
- 20
- Citations by year
- 2025: 3 · 2024: 4 · 2023: 3 · 2022: 1 · 2021: 2 · 2019: 3 · 2018: 4 · 2016: 1 · 2015: 7 · 2014: 6 · 2013: 4
- FWCI
- 4.37
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Licence
- journals.asm.org/non-commercial-tdm-license (tdm)
- Locations
- Journal of Clinical Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Fungal Infections and Studies
- Health Sciences › Medicine › Microbiology (medical) › Bacterial Identification and Susceptibility Testing
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Pathogens and Fungal Diseases
- Keywords
- Cryptococcus neoformans var. grubii, Cryptococcus neoformans var. neoformans, Cryptococcus gattii, interspecific hybrids, species identification, amplicon size, fungal hybridization
- MeSH terms
- Cryptococcosis, Cryptococcus neoformans, DNA, Fungal, Humans, Microbiological Techniques, Molecular Sequence Data, Recombination, Genetic, Time Factors, Polymerase Chain Reaction, Sequence Analysis, DNA, DNA Primers, Molecular Diagnostic Techniques, Cryptococcus gattii
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- CoChat. (2026). Citation check: Development of a Singleplex PCR Assay for Rapid Identification and Differentiation of Cryptococcus neoformans var.grubii, Cryptococcus neoformans var. neoformans, Cryptococcus gattii, and Hybrids. https://app.cochat.ai/papers/10.1128/jcm.00064-13
- The paper
- Feng, X., Fu, X., Ling, B., Wang, L., Liao, W., & Yao, Z. (2013). Development of a Singleplex PCR Assay for Rapid Identification and Differentiation of Cryptococcus neoformans var.grubii, Cryptococcus neoformans var. neoformans, Cryptococcus gattii, and Hybrids. Journal of Clinical Microbiology. https://doi.org/10.1128/jcm.00064-13
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
