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Molecular Typing of IberoAmerican Cryptococcus neoformans Isolates

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Meyer, Wieland, Castañeda, Alexandra, Jackson, Stuart, Huynh, Matthew, Castañeda, Elizabeth, the IberoAmerican Cryptococcal Study Group·2003·Emerging Infectious Diseases

Abstract

A network was established to acquire basic knowledge of Cryptococcus neoformans in IberoAmerican countries. To this effect, 340 clinical, veterinary, and environmental isolates from Argentina, Brazil, Chile, Colombia, Mexico, Peru, Venezuela, Guatemala, and Spain were typed by using M13 polymerase chain reaction-fingerprinting and orotidine monophosphate pyrophosphorylase (URA5) gene restriction fragment length polymorphism analysis with HhaI and Sau96I in a double digest. Both techniques grouped all isolates into eight previously established molecular types. The majority of the isolates, 68.2% (n=232), were VNI (var. grubii, serotype A), which accords with the fact that this variety causes most human cryptococcal infections worldwide. A smaller proportion, 5.6% (n=19), were VNII (var. grubii, serotype A); 4.1% (n=14), VNIII (AD hybrid), with 9 isolates having a polymorphism in the URA5 gene; 1.8% (n=6), VNIV (var. neoformans, serotype D); 3.5% (n=12), VGI; 6.2% (n=21), VGII; 9.1% (n=31), VGIII, and 1.5% (n=5) VGIV, with all four VG types containing var. gatii serotypes B and C isolates.

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Metadata

Authors
Meyer, W., Castañeda, A., Jackson, S., Huynh, M., Castañeda, E., & the IberoAmerican Cryptococcal Study Group
Venue
Emerging Infectious Diseases
Published
2003-02
Volume
9
Issue
2
Pages
189-195
ISSN
  • 1080-6040 (ISSN-L)
  • 1080-6059
OpenAlex
W2091494430
Cited by
520
References
1
FWCI
9.19
Type
journal article
Language
en
Open access
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CoChat. (2026). Citation check: Molecular Typing of IberoAmerican Cryptococcus neoformans Isolates. https://app.cochat.ai/papers/10.3201/eid0902.020246
The paper
Meyer, W., Castañeda, A., Jackson, S., Huynh, M., Castañeda, E., & the IberoAmerican Cryptococcal Study Group. (2003). Molecular Typing of IberoAmerican Cryptococcus neoformans Isolates. Emerging Infectious Diseases. https://doi.org/10.3201/eid0902.020246

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