The DOI matches its Crossref record; 20 of the 20 references it cites were found in a registry or an index.
Comparison of Real-Time PCR Assays with Fluorescent-Antibody Assays for Diagnosis of Respiratory Virus Infections in Children
Abstract
Conventional fluorescent-antibody (FA) methods were compared to real-time PCR assays for detection of respiratory syncytial virus (RSV), influenza virus type A (FluA), parainfluenza virus types 1, 2, and 3 (PIV1, PIV2, and PIV3), human metapneumovirus (MPV), and adenovirus (AdV) in 1,138 specimens from children with respiratory illnesses collected over a 1-year period. At least one virus was detected in 436 (38.3%) specimens by FA and in 608 (53.4%) specimens by PCR ( P < 0.001). Specimen quality was inadequate for FA in 52 (4.6%) specimens; 13 of these (25%) were positive by PCR. In contrast, 18 (1.6%) specimens could not be analyzed by PCR; 1 of these was positive by FA. The number of specimens positive only by PCR among specimens positive by PCR and/or FA was 18 (7.0%) of 257 for RSV, 18 (13.4%) of 134 for FluA, 25 (64.1%) of 39 for PIV1, 8 (88.9%) of 9 for PIV2, 17 (30.1%) of 55 for PIV3, and 101 (76.5%) of 132 for AdV. MPV was detected in 6.6% of all specimens and in 9.5% of the 702 specimens negative by FA. The mean number of virus copies per milliliter in specimens positive by both PCR and FA was significantly higher, at 6.7 × 10 7 , than that in specimens positive only by PCR, at 4.1 × 10 4 ( P < 0.001). The PCR assays were significantly more sensitive than FA assays for detecting respiratory viruses, especially parainfluenza virus and adenovirus. Use of real-time PCR to identify viral respiratory pathogens in children will lead to improved diagnosis of respiratory illness.
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Citations
Cites
Epidemiology of viral respiratory infections
Monto, A. S. (2002) · The American Journal of Medicine · doi:10.1016/s0002-9343(01)01058-0 · Cited by 557
Templeton, K. E., Scheltinga, S. A., Beersma, M. F. C., Kroes, A. C. M., & Claas, E. C. J. (2004) · Journal of Clinical Microbiology · doi:10.1128/jcm.42.4.1564-1569.2004 · Cited by 468
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Development of three multiplex RT-PCR assays for the detection of 12 respiratory RNA viruses
Bellau-Pujol, S., Vabret, A., Legrand, L., Dina, J., Gouarin, S., Petitjean-Lecherbonnier, J., Pozzetto, B., Ginevra, C., & Freymuth, F. (2005) · Journal of Virological Methods · doi:10.1016/j.jviromet.2005.01.020 · Cited by 317
Clinical and Financial Benefits of Rapid Detection of Respiratory Viruses: an Outcomes Study
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Weinberg, G. A., Erdman, D. D., Edwards, K. M., Hall, C. B., Walker, F. J., Griffin, M. R., Schwartz, B., & the New Vaccine Surveillance Network Study Group (2004) · The Journal of Infectious Diseases · doi:10.1086/381456 · Cited by 230
van Gageldonk-Lafeber, A. B., Heijnen, M.-L. A., Bartelds, A. I. M., Peters, M. F., van der Plas, S. M., & Wilbrink, B. (2005) · Clinical Infectious Diseases · doi:10.1086/431982 · Cited by 210
Cited by
Multipathogen infections in hospitalized children with acute respiratory infections
Peng, D., Zhao, D., Liu, J., Wang, X., Yang, K., Xicheng, H., Li, Y., & Wang, F. (2009) · Virology Journal · doi:10.1186/1743-422x-6-155 · Cited by 128
Peddu, V., Shean, R. C., Xie, H., Shrestha, L., Perchetti, G. A., Minot, S. S., Roychoudhury, P., Huang, M.-L., Nalla, A., Reddy, S. B., et al. (2020) · Clinical Chemistry · doi:10.1093/clinchem/hvaa106 · Cited by 73
“Samples were processed and run with EXO as a spiked internal control at a concentration of 1000 copies per lL (9).”
MethodRisk Factors for Hospitalization With Lower Respiratory Tract Infections in Children in Rural Alaska
Bulkow, L. R., Singleton, R. J., DeByle, C., Miernyk, K., Redding, G., Hummel, K. B., Chikoyak, L., & Hennessy, T. W. (2012) · Pediatrics · doi:10.1542/peds.2011-1943 · Cited by 59
Metadata
- Authors
- Kuypers, J., Wright, N., Ferrenberg, J., Huang, M.-L., Cent, A., Corey, L., & Morrow, R.
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2006-07
- Volume
- 44
- Issue
- 7
- Pages
- 2382-2388
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- OpenAlex
- W2167019694
- Cited by
- 323
- References
- 20
- FWCI
- 13.50
- Type
- journal article
- Language
- en
- Open access
- closed
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- Kuypers, J., Wright, N., Ferrenberg, J., Huang, M.-L., Cent, A., Corey, L., & Morrow, R. (2006). Comparison of Real-Time PCR Assays with Fluorescent-Antibody Assays for Diagnosis of Respiratory Virus Infections in Children. Journal of Clinical Microbiology. https://doi.org/10.1128/jcm.00216-06
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