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Cited by 317

The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.

Development of three multiplex RT-PCR assays for the detection of 12 respiratory RNA viruses

Open access
Bellau-Pujol, S., Vabret, A., Legrand, L., Dina, J., Gouarin, S., Petitjean-Lecherbonnier, J., Pozzetto, B., Ginevra, C., Freymuth, F.·2005·Journal of Virological Methods
DOI 10.1016/j.jviromet.2005.01.020
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Abstract

Three multiplex hemi-nested RT-PCR assays were developed to detect simultaneously 12 RNA respiratory viruses: influenza viruses A, B and C, human respiratory syncytial virus (hRSV), human metapneumovirus (hMPV), parainfluenza virus types 1–4 (PIV-1, -2, -3 and -4), human coronavirus OC43 and 229E (HCoV) and rhinovirus (hRV). An internal amplification control was included in one of the RT-PCR assays. The RT-PCR multiplex 1 and the hemi-nested multiplex 1 detected 1 and 0.1 TCID50 of RSV A, respectively, and 0.01 and 0.001 TCID50 of influenza virus A/H3N2, respectively. Two hundred and three nasal aspirates from hospitalised children were retrospectively tested in comparison with two conventional methods: direct immunofluorescence assay and viral isolation technique. Almost all samples (89/91) that were positive by immunofluorescence assay and/or viral isolation technique were detected by the multiplex assay. This method also detected an additional 85 viruses and 33 co-infections. The overall sensitivity (98%), rapidity and enhanced efficiency of these multiplex hemi-nested RT-PCR assays suggest that they would be a significant improvement over conventional methods for the detection of a broad spectrum of respiratory viruses.

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Metadata

Authors
Bellau-Pujol, S., Vabret, A., Legrand, L., Dina, J., Gouarin, S., Petitjean-Lecherbonnier, J., Pozzetto, B., Ginevra, C., & Freymuth, F.
Venue
Journal of Virological Methods
Publisher
Elsevier BV
Published
2005-06
Volume
126
Issue
1-2
Pages
53-63
ISSN
0166-0934 (ISSN-L)
DOI
10.1016/j.jviromet.2005.01.020
OpenAlex
W2063850263
Cited by
317
References
31
FWCI
17.45
Type
journal article
Language
en
Open access
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CoChat. (2026). Citation check: Development of three multiplex RT-PCR assays for the detection of 12 respiratory RNA viruses. https://app.cochat.ai/papers/10.1016/j.jviromet.2005.01.020
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Bellau-Pujol, S., Vabret, A., Legrand, L., Dina, J., Gouarin, S., Petitjean-Lecherbonnier, J., Pozzetto, B., Ginevra, C., & Freymuth, F. (2005). Development of three multiplex RT-PCR assays for the detection of 12 respiratory RNA viruses. Journal of Virological Methods. https://doi.org/10.1016/j.jviromet.2005.01.020

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.