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Comparison of Two Molecular Methods for Rapid Diagnosis of Extrapulmonary Tuberculosis
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Application of real-time PCR for the detection of Mycobacterium tuberculosis enables results to be obtained in about 2 h. A total of 340 nonrespiratory samples were processed using two real-time PCR assay kits: Xpert MTB/RIF and Cobas TaqMan MTB. The sensitivity and specificity of the Xpert assay were 95% and 100%, respectively, compared to 78% and 98% for the Cobas assay.
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“13 studies (839 samples; 10 with .10samples, 159 culture positive) evaluated Xpert in CSF against culture [14-16, 19, 20, 22-25, 27-29, 31].”
From Xpert MTB/RIF assay for the diagnosis of extrapulmonary tuberculosis: a systematic review and meta-analysis (2014) · cites this paper as [16]
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“FNA is increasingly being used worldwide as a first diagnostic step because of its ease and safety profile, although sensitivity is very variable depending on the study, with positive mycobacterial culture ranging from 10% to 80%.3,4,13–16”
From Epidemiology and Diagnosis of Tuberculous Lymphadenitis in a Tuberculosis Low-Burden Country (2015) · cites this paper as [15]
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Metadata
- Authors
- Causse, M., Ruiz, P., Gutiérrez-Aroca, J. B., & Casal, M.
- Affiliations
- Hospital Universitario Reina Sofía (ES)
- University of Córdoba (ES)
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2011-08
- Volume
- 49
- Issue
- 8
- Pages
- 3065-3067
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- PMC
- PMC3147762
- OpenAlex
- W2135290191
- Semantic Scholar
- c1f0f61431f4e037c237ffcb6ced774fc51958b0
- Cited by
- 145
- References
- 14
- Citations by year
- 2024: 4 · 2023: 5 · 2022: 2 · 2021: 15 · 2020: 8 · 2019: 9 · 2018: 8 · 2017: 7 · 2016: 5 · 2015: 19 · 2014: 20 · 2013: 21 · 2012: 16
- FWCI
- 13.59
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Clinical Microbiology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (submittedVersion, cc-by-nc-nd)
- Universidad de Córdoba Insitutional Repository (Universidad de Córdoba) (submittedVersion, cc-by-nc-nd)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Tuberculosis Research and Epidemiology
- Health Sciences › Medicine › Infectious Diseases › Diagnosis and treatment of tuberculosis
- Health Sciences › Medicine › Infectious Diseases › Infectious Diseases and Tuberculosis
- Keywords
- extrapulmonary tuberculosis, Mycobacterium tuberculosis detection, real-time PCR, Xpert MTB/RIF, sensitivity and specificity, rapid molecular diagnostics
- MeSH terms
- Adolescent, Adult, Aged, Aged, 80 and over, Bacteriological Techniques, Child, Child, Preschool, Female, Humans, Male, Middle Aged, Mycobacterium tuberculosis, Sensitivity and Specificity, Tuberculosis, Molecular Diagnostic Techniques, Young Adult
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