Metagenomic Next-Generation Sequencing of Plasma for Diagnosis of COVID-19-Associated Pulmonary Aspergillosis
Open accessAbstract
Timely diagnosis remains an unmet need in non-neutropenic patients at risk for aspergillosis, including those with COVID-19-associated pulmonary aspergillosis (CAPA), which in its early stages is characterized by tissue-invasive growth of the lungs with limited angioinvasion. Currently available mycological tests show limited sensitivity when testing blood specimens.
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There is limited data for the diagnosis of invasive candidiasis by metagenomic next-generation sequencing, although early reports show potential.
“There is limited data thus far for the diagnosis of invasive candidiasis by this methodology, although early reports show potential.24”
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- Authors
- Hoenigl, M., Egger, M., Price, J., Krause, R., Prattes, J., & White, P. L.
- Affiliations
- Medical University of Graz (AT)
- University Hospital of Wales (GB)
- University of Cologne (DE)
- Corresponding author
- Hoenigl, Martin
- Prattes, Juergen
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2023-03-23
- Volume
- 61
- Issue
- 3
- Pages
- e0185922-e0185922
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- PMC
- PMC10035327
- OpenAlex
- W4321446994
- Cited by
- 51
- References
- 32
- Citations by year
- 2026: 6 · 2025: 12 · 2024: 18 · 2023: 15
- FWCI
- 10.56
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Clinical Microbiology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- ORCA Online Research @Cardiff (Cardiff University) (acceptedVersion)
- Funders
- Gilead Sciences (IIR)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Bacterial Identification and Susceptibility Testing
- Health Sciences › Medicine › Infectious Diseases › COVID-19 Clinical Research Studies
- Keywords
- COVID-19-associated pulmonary aspergillosis, metagenomic next-generation sequencing, plasma cell-free DNA, Karius test, Aspergillus fumigatus, Rhizopus microsporus, mold detection, sensitivity and specificity, non-neutropenic patients, intensive care unit patients
- MeSH terms
- Cell-Free Nucleic Acids, COVID-19, Aspergillosis, Female, Humans, Male, Middle Aged
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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