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Molecular analysis of oral and respiratory bacterial species associated with ventilator-associated pneumonia.
Open accessAbstract
Trauma intensive care unit (TICU) patients requiring mechanical respiratory support frequently develop ventilator-associated pneumonia (VAP). Oral and oropharyngeal bacteria are believed to be responsible for many cases of VAP, but definitive evidence of this relationship is lacking. Earlier studies used conventional culture-based methods for identification of bacterial pathogens, but these methods are insufficient, as some bacteria may be uncultivable or difficult to grow. The purpose of this study was to use a culture-independent molecular approach to analyze and compare the bacterial species colonizing the oral cavity and the lungs of TICU patients who developed VAP. Bacterial samples were acquired from the dorsal tongue and bronchoalveolar lavage fluid of 16 patients. Bacterial DNA was extracted, and the 16S rRNA genes were PCR amplified, cloned into Escherichia coli, and sequenced. The sequencing data revealed the following: (i) a wide diversity of bacterial species in both the oral and pulmonary sites, some of them novel; (ii) known and putative respiratory pathogens colonizing both the oral cavity and lungs of 14 patients; and (iii) a number of bacterial pathogens (e.g., Dialister pneumosintes, Haemophilus segnis, Gemella morbillorum, and Pseudomonas fluorescens) in lung samples that had not been reported previously at this site when culture-based methods were used. Our data indicate that the dorsal surface of the tongue serves as a potential reservoir for bacterial species involved in VAP. Furthermore, it is clear that the diversity of bacterial pathogens for VAP is far more complex than the current literature suggests.
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“Several members of the oral microbiota (e.g., Streptococcus oralis, Prevotella salivae, and Mycoplasma salivarium) have previously been detected by 16S rRNA gene analysis in the BALF samples of VAP patients (Bahrani-Mougeot et al., 2007).”
From Oral Microbiome and SARS-CoV-2: Beware of Lung Co-infection (2020) · cites this paper as Bahrani-Mougeot et al., 2007
In the citing paper
“Many studies have reported that the main pathophysiologic mechanism for VAP development is oropharyngeal colonization (Doré et al., 1996; Bahrani-Mougeot et al., 2007).”
From Oral Microbiome and SARS-CoV-2: Beware of Lung Co-infection (2020) · cites this paper as Bahrani-Mougeot et al., 2007
In the citing paper
“in critically ill patients, healthy oral microbiota are displaced by gram-negative aerobes (figure 1), including prominent members of the Proteobacteria phylum”
From The microbiome and critical illness (2016) · cites this paper as [7]
In the citing paper
“becomes overrun by potentially pathogenic bacteria, including prominent Proteobacteria, such as P aeruginosa and K pneumoniae.”
From The microbiome and critical illness (2016) · cites this paper as [7]
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Metadata
- Authors
- Bahrani-Mougeot, F. K., Paster, B. J., Coleman, S., Barbuto, S., Brennan, M. T., Noll, J., Kennedy, T., Fox, P. C., & Lockhart, P. B.
- Affiliations
- Department of Oral Medicine, Carolinas Medical Center, Charlotte, North Carolina
- The Forsyth Institute (US)
- Carolinas Medical Center (US)
- University of Utah (US)
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2007-05
- Volume
- 45
- Issue
- 5
- Pages
- 1588-1593
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- PMC
- PMC1865865
- OpenAlex
- W2069340069
- Cited by
- 176
- References
- 25
- Citations by year
- 2026: 4 · 2025: 4 · 2024: 4 · 2023: 9 · 2022: 10 · 2021: 7 · 2020: 13 · 2019: 8 · 2018: 2 · 2017: 10 · 2016: 10 · 2015: 12 · 2014: 19 · 2013: 13 · 2012: 8
- FWCI
- 9.67
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Clinical Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Critical Care and Intensive Care Medicine › Nosocomial Infections in ICU
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Health Sciences › Medicine › Microbiology (medical) › Bacterial Identification and Susceptibility Testing
- Keywords
- ventilator-associated pneumonia, oral bacteria, oropharyngeal colonization, tongue dorsum, bronchoalveolar lavage, 16S rRNA gene sequencing, bacterial diversity, uncultured bacteria, novel bacterial species, mechanical ventilation, trauma intensive care, bacterial reservoir, Dialister pneumosintes, Gemella morbillorum, Pseudomonas fluorescens
- MeSH terms
- Adult, Bacteria, Female, Humans, Male, Middle Aged, Mouth, Phylogeny, Respiratory System, Species Specificity, Wounds and Injuries, Pneumonia, Bacterial, Pneumonia, Ventilator-Associated
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