The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Strain-Dependent Variation in 18S Ribosomal DNA Copy Numbers in Aspergillus fumigatus
Open accessAbstract
Enumerating Aspergillus fumigatus CFU can be challenging since CFU determination by plate count can be difficult. CFU determination by quantitative real-time PCR (qPCR), however, is becoming increasingly common and usually relies on detecting one of the subunits of the multicopy rRNA genes. This study was undertaken to determine if ribosomal DNA (rDNA) copy number was constant or variable among different A. fumigatus isolates. FKS1 was used as a single-copy control gene and was validated against single-copy ( pyrG and ARG4 ) and multicopy ( arsC ) controls. The copy numbers of the 18S rDNA subunit were then determined for a variety of isolates and were found to vary with the strain, from 38 to 91 copies per genome. Investigation of the stability of the 18S rDNA copy number after exposure to a number of different environmental and growth conditions revealed that the copy number was stable, varying less than one copy across all conditions, including in isolates recovered from an animal model. These results suggest that while the ribosomal genes are excellent targets for enumeration by qPCR, the copy number should be determined prior to using them as targets for quantitative analysis.
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Cites
Aspergillus fumigatus and Aspergillosis
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Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus
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Glucan Synthase Complex of Aspergillus fumigatus
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Attenuated virulence of uridine-uracil auxotrophs of Aspergillus fumigatus
D'Enfert, C., Diaquin, M., Delit, A., Wuscher, N., Debeaupuis, J. P., Huerre, M., & Latge, J. P. (1996) · Infection and Immunity · doi:10.1128/iai.64.10.4401-4405.1996 · Cited by 123
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Carini, P., Marsden, P. J., Leff, J. W., Morgan, E. E., Strickland, M. S., & Fierer, N. (2016) · Nature Microbiology · doi:10.1038/nmicrobiol.2016.242 · Cited by 1,040
Fungal community analysis by high‐throughput sequencing of amplified markers – a user's guide
Lindahl, B. D., Nilsson, R. H., Tedersoo, L., Abarenkov, K., Carlsen, T., Kjøller, R., Kõljalg, U., Pennanen, T., Rosendahl, S., Stenlid, J., et al. (2013) · New Phytologist · doi:10.1111/nph.12243 · Cited by 892
The role of ciliate protozoa in the rumen
Newbold, C. J., de la Fuente, G., Belanche, A., Ramos-Morales, E., & McEwan, N. R. (2015) · Frontiers in Microbiology · doi:10.3389/fmicb.2015.01313 · Cited by 637
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Denning, D. W., Park, S., Lass-Florl, C., Fraczek, M. G., Kirwan, M., Gore, R., Smith, J., Bueid, A., Moore, C. B., Bowyer, P., et al. (2011) · Clinical Infectious Diseases · doi:10.1093/cid/cir179 · Cited by 313
Metadata
- Authors
- Herrera, M. L., Vallor, A. C., Gelfond, J. A., Patterson, T. F., & Wickes, B. L.
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2009-05
- Volume
- 47
- Issue
- 5
- Pages
- 1325-1332
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- OpenAlex
- W2132002261
- Cited by
- 189
- References
- 5
- FWCI
- 6.28
- Type
- journal article
- Language
- en
- Open access
- green
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- Herrera, M. L., Vallor, A. C., Gelfond, J. A., Patterson, T. F., & Wickes, B. L. (2009). Strain-Dependent Variation in 18S Ribosomal DNA Copy Numbers in Aspergillus fumigatus. Journal of Clinical Microbiology. https://doi.org/10.1128/jcm.02073-08
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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