The role of ciliate protozoa in the rumen

Newbold, Charles J.; de la Fuente, Gabriel; Belanche, Alejandro; Ramos-Morales, Eva; McEwan, Neil R.

doi:10.3389/fmicb.2015.01313

Cited by 642Ruminant Nutrition and Digestive PhysiologyAnaerobic Digestion and Biogas ProductionAnimal Nutrition and Physiology

The role of ciliate protozoa in the rumen

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Newbold, Charles J., de la Fuente, Gabriel, Belanche, Alejandro, Ramos-Morales, Eva, McEwan, Neil R.·2015·Frontiers in Microbiology

DOI 10.3389/fmicb.2015.01313PMC PMC4659874

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Abstract

First described in 1843, Rumen protozoa with their striking appearance were assumed to be important for the welfare of their host. However, despite contributing up to 50% of the bio-mass in the rumen, the role of protozoa in rumen microbial ecosystem remains unclear. Phylogenetic analysis of 18S rDNA libraries generated from the rumen of cattle, sheep, and goats has revealed an unexpected diversity of ciliated protozoa although variation in gene copy number between species makes it difficult to obtain absolute quantification. Despite repeated attempts it has proven impossible to maintain rumen protozoa in axenic culture. Thus it has been difficult to establish conclusively a role of ciliate protozoa in rumen fiber degradation. The development of techniques to clone and express ciliate genes in λ phage, together with bioinformatic indices to confirm the ciliate origin of the genes has allowed the isolation and characterization of fibrolytic genes from rumen protozoa. Elimination of the ciliate protozoa increases microbial protein supply by up to 30% and reduces methane production by up to 11%. Our recent findings suggest that holotrich protozoa play a disproportionate role in supporting methanogenesis whilst the small Entodinium are responsible for much of the bacterial protein turnover. As yet no method to control protozoa in the rumen that is safe and practically applicable has been developed, however a range of plant extract capable of controlling if not completely eliminating rumen protozoa have been described.

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Rumen bacteria play a dominant role in fiber digestion, although anaerobic fungi and protozoa contribute to lignocellulose breakdown by attacking lignocellulosic material differently and discharging different enzyme complexes.
“Rumen bacteria play a dominant role in fiber digestion, although anaerobic fungi and protozoa have been reported to contribute to lignocellulose breakdown by attacking lignocellulosic material differently (60, 64) and discharging different enzyme complexes (101).”
From Phytogenic Additives Can Modulate Rumen Microbiome to Mediate Fermentation Kinetics and Methanogenesis Through Exploiting Diet–Microbe Interaction (2020) · cites this paper as [64]
Citation (101) is attached to the claim about discharging different enzyme complexes. The ‘Functional Genes in Rumen Ciliate Protozoa’ section reports a wide range of protozoal fibrolytic enzymes, but does not establish that fungi and protozoa discharge different enzyme complexes. (LLM, conf=0.83)
Experimental — accuracy not yet measured
Defaunation reduces methane output by approximately 11%.
“protozoa have been linked to methanogenesis as defaunation reduces methane output by approximately 11% (Hegarty, 1999; Morgavi et al., 2010; Newbold et al., 2015).”
From Addressing global ruminant agricultural challenges through understanding the rumen microbiome: past, present, and future (2018) · cites this paper as Newbold et al., 2015
Removal of protozoa from the rumen causes a decrease in organic matter degradation, especially of neutral and acid detergent fiber.
“Based on their analysis, Newbold and colleagues found evidence that the removal of protozoa from the rumen caused a decrease in organic matter degradation, especially of neutral and acid detergent fiber.”
From Addressing global ruminant agricultural challenges through understanding the rumen microbiome: past, present, and future (2018) · cites this paper as Newbold et al., 2015

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Development and Validation of a Real-Time PCR Method To Quantify Rumen Protozoa and Examination of Variability between Entodinium Populations in Sheep Offered a Hay-Based Diet
Skillman, L. C., Toovey, A. F., Williams, A. J., & Wright, A. G. (2006) · Applied and Environmental Microbiology · doi:10.1128/aem.72.1.200-206.2006 · Cited by 78 · see citing works
RNA-Mediated Epigenetic Programming of Genome Rearrangements
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Exposure to Rumen Protozoa Leads to Enhancement of Pathogenicity of and Invasion by Multiple-Antibiotic-ResistantSalmonella entericaBearing SGI1
Rasmussen, M. A., Carlson, S. A., Franklin, S. K., McCuddin, Z. P., Wu, M. T., & Sharma, V. K. (2005) · Infection and Immunity · doi:10.1128/iai.73.8.4668-4675.2005 · Cited by 76 · see citing works
Changes in methane emission and rumen fermentation parameters induced by refaunation in sheep
Morgavi, D. P., Jouany, J. P., & Martin, C. (2008) · Australian Journal of Experimental Agriculture · doi:10.1071/ea07236 · Cited by 75 · see citing works
Effect of progressive inoculation of fauna-free sheep with holotrich protozoa and total-fauna on rumen fermentation, microbial diversity and methane emissions
Belanche, A., de la Fuente, G., & Newbold, C. J. (2014) · FEMS Microbiology Ecology · doi:10.1093/femsec/fiu026 · Cited by 74 · see citing works
Effect of defaunation on the loss of energy in wethers fed different quantities of cellulose and normal or steamflaked maize starch
Kreuzer, M., Kirchgeßner, M., & Muller, H. L. (1986) · Animal Feed Science and Technology · doi:10.1016/0377-8401(86)90114-8 · Cited by 74 · see citing works
Contribution of rumen protozoa to duodenal flow of nitrogen, conjugated linoleic acid and vaccenic acid in steers fed silages differing in their water-soluble carbohydrate content
Yáñez-Ruíz, D. R., Scollan, N. D., Merry, R. J., & Newbold, C. J. (2006) · British Journal Of Nutrition · doi:10.1017/bjn20061927 · Cited by 72 · see citing works
Structures of free-living and protozoa-associated methanogen communities in the bovine rumen differ according to comparative analysis of 16S rRNA and mcrA genes
Tymensen, L. D., Beauchemin, K. A., & McAllister, T. A. (2012) · Microbiology · doi:10.1099/mic.0.057984-0 · Cited by 69 · see citing works
Phylogeny of Intestinal Ciliates, Including Charonina ventriculi, and Comparison of Microscopy and 18S rRNA Gene Pyrosequencing for Rumen Ciliate Community Structure Analysis
Kittelmann, S., Devente, S., Kirk, M. R., Seedorf, H., Dehority, B. A., & Janssen, P. H. (2015) · Applied and Environmental Microbiology · doi:10.1128/aem.03697-14 · Cited by 67 · see citing works
A xylanase produced by the rumen anaerobic protozoan Polyplastron multivesiculatum shows close sequence similarity to family 11 xylanases from Gram-positive bacteria
Devillard, E., Newbold, C. J., Scott, K. P., Forano, É., Wallace, R. J., Jouany, J. P., & Flint, H. J. (1999) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1999.tb08837.x · Cited by 67 · see citing works
The pentosanases of some rumen bacteria
Howard, B. H., Jones, G., & Purdom, M. R. (1960) · Biochemical Journal · doi:10.1042/bj0740173 · Cited by 66 · see citing works
Methanogens associated with rumen ciliates
Tokura, M., Ushida, K., Miyazaki, K., & KOJIMA, Y. (2006) · FEMS Microbiology Ecology · doi:10.1111/j.1574-6941.1997.tb00365.x · Cited by 66 · see citing works
Xylanases and carboxymethylcellulases of the rumen protozoaPolyplastron multivesiculatum,Eudiplodinium maggiiandEntodiniumsp.
Béra‐Maillet, C., Devillard, E., Cezette, M., Jouany, J. P., & Forano, É. (2005) · FEMS Microbiology Letters · doi:10.1016/j.femsle.2005.01.035 · Cited by 65 · see citing works
Investigating unsaturated fat, monensin, or bromoethanesulfonate in continuous cultures retaining ruminal protozoa. I. Fermentation, biohydrogenation, and microbial protein synthesis
Karnati, S. K. R., Sylvester, J. T., Ribeiro, C. V. D. M., Gilligan, L. E., & Firkins, J. L. (2009) · Journal of Dairy Science · doi:10.3168/jds.2008-1436 · Cited by 61 · see citing works
Characterization of XYN10B, a modular xylanase from the ruminal protozoan Polyplastron multivesiculatum, with a family 22 carbohydrate-binding module that binds to cellulose
Devillard, E., Béra‐Maillet, C., Flint, H. J., Scott, K. P., Newbold, C. J., Wallace, R. J., Jouany, J. P., & Forano, É. (2003) · Biochemical Journal · doi:10.1042/bj20021784 · Cited by 60 · see citing works
Activity-Based Metagenomic Screening and Biochemical Characterization of Bovine Ruminal Protozoan Glycoside Hydrolases
Findley, S. D., Mormile, M. R., Hurley-Sommer, A., Zhang, X., Tipton, P. A., Arnett, K. L., Porter, J. H., Kerley, M. S., & Stacey, G. S. (2011) · Applied and Environmental Microbiology · doi:10.1128/aem.05925-11 · Cited by 60 · see citing works
Monophyly of the Trichostome ciliates (Phylum ciliophora: Class litostomatea) tested using new 18S rRNA sequences from the Vestibuliferids, Isotricha intestinalis and Dasytricha ruminantium, and the Haptorian, Didinium nasutum
Wright, A. G., & Lynn, D. H. (1997) · European Journal of Protistology · doi:10.1016/s0932-4739(97)80008-9 · Cited by 57 · see citing works
Rumen ciliated protozoa decrease generation time and adjust 18S ribosomal DNA copies to adapt to decreased transfer interval, starvation, and monensin
Sylvester, J. T., Karnati, S. K. R., Dehority, B. A., Morrison, M., Smith, G. L., St-Pierre, N. R., & Firkins, J. L. (2008) · Journal of Dairy Science · doi:10.3168/jds.2008-1417 · Cited by 57 · see citing works
The symbiotic intestinal ciliates and the evolution of their hosts
der Staay, S. Y. M., van der Staay, G. W. M., Michałowski, T., Jouany, J. P., Pristaš, P., Javorský, P., Kišidayová, S., Váradyová, Z., McEwan, N. R., Newbold, C. J., et al. (2014) · European Journal of Protistology · doi:10.1016/j.ejop.2014.01.004 · Cited by 57 · see citing works
Hydrogenosomes in the rumen entodiniomorphid ciliate Polyplastron multivesiculatum
Paul, R. G., Williams, A. G., & Butler, R. D. (1990) · Journal of General Microbiology · doi:10.1099/00221287-136-10-1981 · Cited by 54 · see citing works
Study of the effect of presence or absence of protozoa on rumen fermentation and microbial protein contribution to the chyme1
Belanche, A., Abecia, L., Holtrop, G., Guada, J. A., Castrillo, C., de la Fuente, G., & Balcells, J. (2011) · Journal of Animal Science · doi:10.2527/jas.2010-3703 · Cited by 54 · see citing works
Effects of feeding lauric acid or coconut oil on ruminal protozoa numbers, fermentation pattern, digestion, omasal nutrient flow, and milk production in dairy cows
Faciola, A. P., & Broderick, G. A. (2014) · Journal of Dairy Science · doi:10.3168/jds.2013-7653 · Cited by 53 · see citing works
Fiber Digestion by Rumen Ciliate Protozoa
Takénaka, A., Tajima, K., Mitsumori, M., & Kajikawa, H. (2004) · Microbes and Environments · doi:10.1264/jsme2.19.203 · Cited by 49 · see citing works
A re-appraisal of the diversity of the methanogens associated with the rumen ciliates
REGENSBOGENOVA, M., McEwan, N. R., JAVORSKY, P., Kišidayová, S., Michałowski, T., Newbold, C. J., Hackstein, J. H. P., & Pristaš, P. (2004) · FEMS Microbiology Letters · doi:10.1016/j.femsle.2004.07.049 · Cited by 49 · see citing works
Effect of defaunation on protein and fibre digestion in sheep fed on ammonia-treated straw-based diets with or without maize
USHIDA, K., Kayouli, C., SMET, S. D., & Jouany, J. P. (1990) · British Journal Of Nutrition · doi:10.1079/bjn19900078 · Cited by 48 · see citing works
Phylogenetic analysis of the rumen ciliate family Ophryoscolecidae based on 18S ribosomal RNA sequences, with new sequences fromDiplodinium,Eudiplodinium, andOphryoscolex
Wright, A. G., & Lynn, D. H. (1997) · Canadian Journal of Zoology · doi:10.1139/z97-117 · Cited by 48 · see citing works
Design and Validation of Four New Primers for Next-Generation Sequencing To Target the 18S rRNA Genes of Gastrointestinal Ciliate Protozoa
Ishaq, S. L., & Wright, A. G. (2014) · Applied and Environmental Microbiology · doi:10.1128/aem.01644-14 · Cited by 48 · see citing works
Persistence of defaunation effects on digestion and methane production in ewes
Bird, S. H., Hegarty, R. S., & Woodgate, R. T. (2008) · Australian Journal of Experimental Agriculture · doi:10.1071/ea07298 · Cited by 47 · see citing works
Carbohydrases of the rumen ciliate Epidinium ecaudatum (Crawley)
BAILEY, R., Clarke, R. T. J., & Wright, D. E. (1962) · Biochemical Journal · doi:10.1042/bj0830517 · Cited by 47 · see citing works
A re-appraisal of the diversity of the methanogens associated with the rumen ciliates
Regensbogenova, M., McEwan, N. R., Javorsky, P., Kišidayová, S., Michałowski, T., Newbold, C. J., Hackstein, J. H. P., & Pristaš, P. (2004) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.2004.tb09771.x · Cited by 47 · see citing works
Effects of the absence of protozoa from birth or from weaning on the growth and methane production of lambs
Hegarty, R. S., Bird, S. H., Vanselow, B. A., & Woodgate, R. T. (2008) · British Journal Of Nutrition · doi:10.1017/s0007114508981435 · Cited by 46 · see citing works
Ruminal fermentation and duodenal flow following progressive inoculations of fauna-free wethers with major individual species of ciliate protozoa or total fauna.
Ivan, M., Neill, L., & Entz, T. (2000) · Journal of Animal Science · doi:10.2527/2000.783750x · Cited by 44 · see citing works
Rumen protozoa are rich in polyunsaturated fatty acids due to the ingestion of chloroplasts
Huws, S., Kim, E. J., Kingston‐Smith, A. H., Lee, M. R. F., Muetzel, S., Cookson, A., Newbold, C. J., Wallace, R. J., & Scollan, N. D. (2009) · FEMS Microbiology Ecology · doi:10.1111/j.1574-6941.2009.00717.x · Cited by 43 · see citing works
Rumen microbial population dynamics in response to photoperiod
McEwan, N. R., Abecia, L., Regensbogenova, M., Adam, C. L., Findlay, P. A., & Newbold, C. J. (2005) · Letters in Applied Microbiology · doi:10.1111/j.1472-765x.2005.01707.x · Cited by 43 · see citing works
Assessment of Ruminal Bacterial Populations and Protozoal Generation Time in Cows Fed Different Methionine Sources
Karnati, S. K. R., Sylvester, J. T., Noftsger, S. M., Yu, Z., St-Pierre, N. R., & Firkins, J. L. (2007) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(07)71564-3 · Cited by 43 · see citing works
The effect of absence of protozoa on rumen biohydrogenation and the fatty acid composition of lamb muscle
Yáñez-Ruíz, D. R., Williams, S., & Newbold, C. J. (2007) · British Journal Of Nutrition · doi:10.1017/s0007114507675187 · Cited by 43 · see citing works
Effect of diet and absence of protozoa on the rumen microbial community and on the representativeness of bacterial fractions used in the determination of microbial protein synthesis1
Belanche, A., de la Fuente, G., Pinloche, E., Newbold, C. J., & Balcells, J. (2012) · Journal of Animal Science · doi:10.2527/jas.2011-4802 · Cited by 42 · see citing works
Rumen Ciliate Protozoa of the Blue Duiker (Cephalophus monticola), with Observations on Morphological Variation Lines Within the Species Entodinium dubardi
Dehority, B. A. (1994) · Journal of Eukaryotic Microbiology · doi:10.1111/j.1550-7408.1994.tb01481.x · Cited by 42 · see citing works
Myristic acid supports the immediate inhibitory effect of lauric acid on ruminal methanogens and methane release
Soliva, C. R., Meile, L., Hindrichsen, I. K., Kreuzer, M., & Machmüller, A. (2004) · Anaerobe · doi:10.1016/j.anaerobe.2004.06.003 · Cited by 42 · see citing works
Relative diversity and community structure analysis of rumen protozoa according to T-RFLP and microscopic methods
Tymensen, L. D., Barkley, C., & McAllister, T. A. (2011) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2011.09.005 · Cited by 40 · see citing works
Rapid Identification of Rumen Protozoa by Restriction Analysis of Amplified 18S rRNA Gene
Regensbogenova, M., Kišidayová, S., Michałowski, T., Javorský, P., der Staay, S. Y. M., van der Staay, G. W. M., Hackstein, J. H. P., McEwan, N. R., Jouany, J. P., Newbold, C. J., et al. (2004) · Radboud Repository (Radboud University) · Cited by 40 · see citing works
Interspecies hydrogen transfer between the rumen ciliate Polyplastron multivesiculatum and Methanosarcina barkeri.
Ushida, K., Newbold, C. J., & Jouany, J.-P. (1997) · The Journal of General and Applied Microbiology · doi:10.2323/jgam.43.129 · Cited by 39 · see citing works
The [FeFe] hydrogenase of Nyctotherus ovalis has a chimeric origin
Boxma, B., Ricard, G., van Hoek, A. H., Severing, E. I., der Staay, S.-Y. M.-V., van der Staay, G. W. M., van Alen, T. A., de Graaf, R. M., Cremers, G., Kwantes, M., et al. (2007) · BMC Evolutionary Biology · doi:10.1186/1471-2148-7-230 · Cited by 39 · see citing works
Involvement of aSalmonellaGenomic Island 1 Gene in the Rumen Protozoan-Mediated Enhancement of Invasion for Multiple-Antibiotic-ResistantSalmonella entericaSerovar Typhimurium
Carlson, S. A., Sharma, V. K., McCuddin, Z. P., Rasmussen, M. A., & Franklin, S. K. (2006) · Infection and Immunity · doi:10.1128/iai.00679-06 · Cited by 39 · see citing works
The effects of defaunation on the metabolism of lactic acid in the rumen
Newbold, C. J., Chamberlain, D. G., & Williams, A. G. (1986) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.2740371105 · Cited by 38 · see citing works
Reponses of sheep to a vaccination of entodinial or mixed rumen protozoal antigens to reduce rumen protozoal numbers
Williams, Y. J., Rea, S. M., Popovski, S., Pimm, C. L., Williams, A. J., Toovey, A. F., Skillman, L. C., & Wright, A. G. (2007) · British Journal Of Nutrition · doi:10.1017/s0007114507801553 · Cited by 37 · see citing works
Accumulation of Reserve Carbohydrate by Rumen Protozoa and Bacteria in Competition for Glucose
Denton, B. L., Diese, L. E., Firkins, J. L., & Hackmann, T. J. (2014) · Applied and Environmental Microbiology · doi:10.1128/aem.03736-14 · Cited by 36 · see citing works
Attempted induction of reductive acetogenesis into the rumen fermentation in vitro
Demeyer, D., Fiedler, D., & Graeve, K. D. (1996) · annales de biologie animale biochimie biophysique · doi:10.1051/rnd:19960301 · Cited by 30 · see citing works
Isotrichid protozoa influence conversion of glucose to glycogen and other microbial products
Hall, M. B. (2011) · Journal of Dairy Science · doi:10.3168/jds.2010-3878 · Cited by 30 · see citing works
Effects of defaunation and various nitrogen supplementation regimens on microbial numbers and activity in the rumen of sheep.
Hsu, J., Fahey, G. C., Jr., Merchen, N. R., & Mackie, R. I. (1991) · Journal of Animal Science · doi:10.2527/1991.6931279x · Cited by 29 · see citing works
Rate of production of protozoa in the rumen and the flow of protozoal nitrogen to the duodenum in sheep and cattle given a pelleted diet of lucerne hay and barley
Punia, B. S., Leibholz, J., & Faichney, G. (1992) · The Journal of Agricultural Science · doi:10.1017/s0021859600068830 · Cited by 27 · see citing works
The effects of protozoa and of supplementation with nitrogen and sulfur on digestion and microbial metabolism in the rumen of sheep
Hegarty, R., Nolan, J. V., & Leng, R. A. (1994) · Australian Journal of Agricultural Research · doi:10.1071/ar9941215 · Cited by 23 · see citing works
Phylogenetic position and codon usage of two centrin genes from the rumen ciliate protozoan, Entodinium caudatum
Eschenlauer, S. C. P., McEwan, N. R., Calza, R. E., Wallace, R. J., Onodera, R., & Newbold, C. J. (1998) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1998.tb13196.x · Cited by 23 · see citing works
Molecular cloning, expression, and characterization of an endo-.BETA.-1,4-glucanase cDNA from Epidinium caudatum.
Takénaka, A., D'Silva, C. G., Kudo, H., ITABASHI, H., & Cheng, K. J. (1999) · The Journal of General and Applied Microbiology · doi:10.2323/jgam.45.57 · Cited by 22 · see citing works
Rumen characteristics, methane emissions, and digestion in weaned lambs reared in isolation
Faichney, G. J., Graham, N., & Walker, D. (1999) · Australian Journal of Agricultural Research · doi:10.1071/ar98195 · Cited by 22 · see citing works
Effects of Daily and Interval Feeding of Sapindus rarak Saponins on Protozoa, Rumen Fermentation Parameters and Digestibility in Sheep
Wina, E., Muetzel, S., & Becker, K. (2006) · Asian-Australasian Journal of Animal Sciences · doi:10.5713/ajas.2006.1580 · Cited by 21 · see citing works
ORIGINAL ARTICLE: Description of development of rumen ecosystem by PCR assay in milk-fed, weaned and finished lambs in an intensive fattening system
Belanche, A., Balcells, J., de la Fuente, G., Yáñez-Ruíz, D. R., Fondevila, M., & Calleja, L. (2009) · Journal of Animal Physiology and Animal Nutrition · doi:10.1111/j.1439-0396.2009.00952.x · Cited by 20 · see citing works
Modeling the distribution of ciliate protozoa in the reticulo-rumen using linear programming
Hook, S. E., Dijkstra, J., Wright, A.-D. G., McBride, B. W., & France, J. (2011) · Journal of Dairy Science · doi:10.3168/jds.2011-4352 · Cited by 19 · see citing works
An NAD+-dependent glutamate dehydrogenase cloned from the ruminal ciliate protozoan,Entodinium caudatum
Newbold, C. J., McEwan, N. R., Calza, R. E., Chareyron, E. N., Duval, S. M., Eschenlauer, S. C. P., McIntosh, F. M., Nelson, N., Travis, A. J., & Wallace, R. J. (2005) · FEMS Microbiology Letters · doi:10.1016/j.femsle.2005.04.034 · Cited by 19 · see citing works
Fluorescence in situ hybridization probing of protozoal Entodinium spp. and their methanogenic colonizers in the rumen of cattle fed alfalfa hay or triticale straw
Xia, Y. L., Kong, Y., Seviour, R. J., Forster, R. J., Kišidayová, S., & McAllister, T. A. (2013) · Journal of Applied Microbiology · doi:10.1111/jam.12356 · Cited by 19 · see citing works
Improved in vitro procedure for maintaining stock cultures of three genera of rumen protozoa1
Dehority, B. A. (2008) · Journal of Animal Science · doi:10.2527/jas.2007-0238 · Cited by 18 · see citing works
Characterization of the amylolytic properties of the rumen ciliate protozoan Eudiplodinium maggii
Bełżecki, G., Newbold, C. J., McEwan, N. R., McIntosh, F. M., & Michałowski, T. (2007) · Journal of Animal and Feed Sciences · doi:10.22358/jafs/66817/2007 · Cited by 16 · see citing works
Comparison of duodenal flow and digestibility in fauna-free sheep inoculated with Holotrich protozoa,Entodiniummonofauna or total mixed protozoa population
Ivan, M. (2008) · British Journal Of Nutrition · doi:10.1017/s0007114508984245 · Cited by 16 · see citing works
Ruminal protozoal contribution to the duodenal flow of fatty acids following feeding of steers on forages differing in chloroplast content
Huws, S., Lee, M. R. F., Kingston‐Smith, A. H., Kim, E. J., Scott, M. B., Tweed, J. K. S., & Scollan, N. D. (2012) · British Journal Of Nutrition · doi:10.1017/s0007114512000335 · Cited by 16 · see citing works
Rumen protozoal diversity in the Spanish ibex (Capra pyrenaica hispanica) as compared with domestic goats (Capra hircus)
de la Fuente, G., Belanche, A., Abecia, L., Dehority, B. A., & Fondevila, M. (2008) · European Journal of Protistology · doi:10.1016/j.ejop.2008.07.001 · Cited by 14 · see citing works
Effect of supplementing coconut or krabok oil, rich in medium-chain fatty acids on ruminal fermentation, protozoa and archaeal population of bulls
Panyakaew, P., Boon, N., Goel, G., Yuangklang, C., Schonewille, J. T., Hendriks, W. H., & Fievez, V. (2013) · animal · doi:10.1017/s1751731113001766 · Cited by 14 · see citing works
Hydrolytic activities of rumen ciliates.
Delfosse-Debusscher, J., Hoof, F. V., Hellings, P., & Thines-Sempoux, D. (1979) · PubMed · Cited by 14 · see citing works
Effects of Rumen Protozoa on Energy Utilization by Wethers of Two Diets Based on Ammonia-Treated Straw Supplemented or not with Maize
Vermorel, M., & Jouany, J. P. (1989) · Asian-Australasian Journal of Animal Sciences · doi:10.5713/ajas.1989.475 · Cited by 12 · see citing works
Effect of Rumen Protozoa onEscherichia coliO157:H7 in the Rumen and Feces of Specifically Faunated Sheep
Stanford, K., Bach, S. J., Stephens, T. P., & McAllister, T. A. (2010) · Journal of Food Protection · doi:10.4315/0362-028x-73.12.2197 · Cited by 12 · see citing works
Use of quantitative real-time PCR to assess thein vitrosurvival of specific DNA gene sequences of rumen microbes under simulated abomasal conditions
Belanche, A., Erroa, I. R., Balcells, J., & Calleja, L. (2009) · Journal of Animal Physiology and Animal Nutrition · doi:10.1111/j.1439-0396.2008.00901.x · Cited by 9 · see citing works
Effects of wortmannin, sodium nitroprusside, insulin, genistein, and guanosine triphosphate on chemotaxis and cell growth of Entodinium caudatum, Epidinium caudatum, and mixed ruminal protozoa
Díaz, H. L., Knapp, J. R., Karnati, S. K. R., Dehority, B. A., & Firkins, J. L. (2014) · Journal of Dairy Science · doi:10.3168/jds.2013-7429 · Cited by 9 · see citing works
A cellulase produced by the rumen protozoan Epidinium ecaudatum is of bacterial origin and has an unusual pH optimum
Wereszka, K., McIntosh, F. M., Michałowski, T., Jouany, J. P., Nsabimana, E., Machebœuf, D., McEwan, N. R., & Newbold, C. J. (2004) · HAL · Cited by 9 · see citing works
Effects of lysine level and Na+K-Cl ratio on lusine-arginine antagonism, blood pH, blood acid-base parameters and growth performance in broiler chicks
Kim, H. W., Han, I. K., & Choi, Y. J. (1989) · Asian-Australasian Journal of Animal Sciences · doi:10.5713/ajas.1989.7 · Cited by 8 · see citing works
Protozoal Sequences may Reveal Additional Isoforms of the 14-3-3 Protein Family
McEwan, N. R., Eschenlauer, S. C. P., Calza, R. E., Wallace, R. J., & Newbold, C. J. (1999) · Protist · doi:10.1016/s1434-4610(99)70028-3 · Cited by 7 · see citing works
An Unusual Codon Usage Pattern in the Ciliate Family Ophryoscolecidae and its Implications for Determining the Source of Cloned DNA
McEwan, N. R., Gatherer, D., Eschenlauer, S. C. P., McIntosh, F. M., Calza, R. E., Wallace, R. J., & Newbold, C. J. (2000) · Anaerobe · doi:10.1006/anae.1999.0310 · Cited by 7 · see citing works
Energy Metabolism and Methane Production in Faunated and Defaunated Sheep Fed Two Diets with Same Concentrate to Roughage Ratio (70:30) but Varying in Composition
Chandramoni, Jadhao, S. B., Tiwari, C. M., Haque, N., Murarilal, & Khan, M. (2001) · Asian-Australasian Journal of Animal Sciences · doi:10.5713/ajas.2001.1238 · Cited by 7 · see citing works
Non-coding RNAs mediate the rearrangements of genomic DNA in ciliates
Feng, X., & Guang, S. (2013) · Science China Life Sciences · doi:10.1007/s11427-013-4539-4 · Cited by 5 · see citing works
Chromatographic separation of some cell wall polysaccharide-degrading enzymes of the sheep rumen Ciliate Epidinium caudatum
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Mechanism of cellulose degradation by the rumen ciliates.
Thinessempoux, D., Delfosse-Debusscher, J., & Latteur, B. (1980) · Digital Access to Libraries · Cited by 4 · see citing works
Studies on the variation in caudal spination of Epidinium
Dehority, B. A. (2006) · Zootaxa · doi:10.11646/zootaxa.1305.1.3 · Cited by 3 · see citing works
The effect of absence of protozoa on methane emissions by lambs
Yáñez-Ruíz, D. R., Hart, K. J., Belanche, A., Martín-García, A. I., & Newbold, C. J. (2007) · Proceedings of the British Society of Animal Science · doi:10.1017/s1752756200019505 · Cited by 2 · see citing works

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Sorted by citations

The rumen microbiome: balancing food security and environmental impacts
Mizrahi, I., Wallace, R. J., & Moraïs, S. (2021) · Nature Reviews Microbiology · doi:10.1038/s41579-021-00543-6 · Cited by 490 · see citing works
Addressing global ruminant agricultural challenges through understanding the rumen microbiome: past, present, and future
Huws, S. A., Creevey, C. J., Oyama, L. B., Mizrahi, I., Denman, S. E., Popova, M., Muñoz-Tamayo, R., Forano, E., Waters, S. M., Hess, M., et al. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.02161 · Cited by 487 · see citing works
Self-citation
The rumen microbiome: a crucial consideration when optimising milk and meat production and nitrogen utilisation efficiency
Matthews, C., Crispie, F., Lewis, E., Reid, M., O’Toole, P. W., & Cotter, P. D. (2018) · Gut Microbes · doi:10.1080/19490976.2018.1505176 · Cited by 466 · see citing works
Invited review: Plant polyphenols and rumen microbiota responsible for fatty acid biohydrogenation, fiber digestion, and methane emission: Experimental evidence and methodological approaches
Vasta, V., Daghio, M., Cappucci, A., Buccioni, A., Serra, A., Viti, C., & Mele, M. L. (2019) · Journal of Dairy Science · doi:10.3168/jds.2018-14985 · Cited by 432 · see citing works
A heritable subset of the core rumen microbiome dictates dairy cow productivity and emissions
Wallace, R. J., Sasson, G., Garnsworthy, P. C., Tapio, I., Gregson, E., Bani, P., Huhtanen, P., Bayat, A. R., Strozzi, F., Biscarini, F., et al. (2019) · Science Advances · doi:10.1126/sciadv.aav8391 · Cited by 410 · see citing works
The ruminal microbiome associated with methane emissions from ruminant livestock
Tapio, I., Snelling, T. J., Strozzi, F., & Wallace, R. J. (2017) · Journal of Animal Science and Biotechnology · doi:10.1186/s40104-017-0141-0 · Cited by 391 · see citing works
Invited review: current enteric methane mitigation options
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Review: Ruminal microbiome and microbial metabolome: effects of diet and ruminant host
Newbold, C. J., & Ramos‐Morales, E. (2020) · animal · doi:10.1017/s1751731119003252 · Cited by 279 · see citing works
The host-associated archaeome
Borrel, G., Brugère, J., Gribaldo, S., Schmitz, R. A., & Moissl‐Eichinger, C. (2020) · Nature Reviews Microbiology · doi:10.1038/s41579-020-0407-y · Cited by 255 · see citing works
The Role of the Gut Microbiome in Cattle Production and Health: Driver or Passenger?
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Silage review: Mycotoxins in silage: Occurrence, effects, prevention, and mitigation
Ogunade, I. M., Tuppia, C. M., Queiroz, O. C. M., Jiang, Y., Drouin, P., Wu, F., Vyas, D., & Adesogan, A. T. (2018) · Journal of Dairy Science · doi:10.3168/jds.2017-13788 · Cited by 248 · see citing works
Degradation of Cellulose and Hemicellulose by Ruminal Microorganisms
Weimer, P. J. (2022) · Microorganisms · doi:10.3390/microorganisms10122345 · Cited by 218 · see citing works
Ruminal methane production: Associated microorganisms and the potential of applying hydrogen-utilizing bacteria for mitigation
Lan, W., & Yang, C. (2018) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2018.11.180 · Cited by 211 · see citing works
Tannin in Ruminant Nutrition: Review
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Associations between Gut Microbiota and Common Luminal Intestinal Parasites
Stensvold, C. R., & van der Giezen, M. (2018) · Trends in Parasitology · doi:10.1016/j.pt.2018.02.004 · Cited by 188 · see citing works
Whole rumen metagenome sequencing allows classifying and predicting feed efficiency and intake levels in cattle
Delgado, B., Bach, À., Guasch, I., González, C., Elcoso, G., Pryce, J. E., & González-Recio, Ó. (2019) · Scientific Reports · doi:10.1038/s41598-018-36673-w · Cited by 186 · see citing works
Seaweed and Seaweed Bioactives for Mitigation of Enteric Methane: Challenges and Opportunities
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Metatranscriptomics Reveals the Active Bacterial and Eukaryotic Fibrolytic Communities in the Rumen of Dairy Cow Fed a Mixed Diet
Comtet-Marre, S., Parisot, N., Lepercq, P., Chaucheyras‐Durand, F., Mosoni, P., Peyretaillade, É., Bayat, A. R., Shingfield, K. J., Peyret, P., & Forano, É. (2017) · Frontiers in Microbiology · doi:10.3389/fmicb.2017.00067 · Cited by 183 · see citing works
Invited review: Large-scale indirect measurements for enteric methane emissions in dairy cattle: A review of proxies and their potential for use in management and breeding decisions
Negussie, E., de Haas, Y., Dehareng, F., Dewhurst, R. J., Dijkstra, J., Gengler, N., Morgavi, D. P., Soyeurt, H., van Gastelen, S., Yan, T., et al. (2017) · Journal of Dairy Science · doi:10.3168/jds.2016-12030 · Cited by 174 · see citing works
Phytogenic Additives Can Modulate Rumen Microbiome to Mediate Fermentation Kinetics and Methanogenesis Through Exploiting Diet–Microbe Interaction
Hassan, F.-U., Arshad, M. A., Ebeid, H. M., Rehman, M. S.-U., Khan, M. S., Shahid, S., & Yang, C. (2020) · Frontiers in Veterinary Science · doi:10.3389/fvets.2020.575801 · Cited by 160 · see citing works
Islands in the stream: from individual to communal fiber degradation in the rumen ecosystem
Moraïs, S., & Mizrahi, I. (2019) · FEMS Microbiology Reviews · doi:10.1093/femsre/fuz007 · Cited by 155 · see citing works
Promising biological conversion of lignocellulosic biomass to renewable energy with rumen microorganisms: A comprehensive review
Liang, J., Nabi, M., Zhang, P., Zhang, G., Cai, Y., Wang, Q., Zhou, Z., & Ding, Y. (2020) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2020.110335 · Cited by 158 · see citing works
Rumen Microbiome Composition Is Altered in Sheep Divergent in Feed Efficiency
McLoughlin, S., Spillane, C., Claffey, N. A., Smith, P. E., O’Rourke, T., Diskin, M. G., & Waters, S. M. (2020) · Frontiers in Microbiology · doi:10.3389/fmicb.2020.01981 · Cited by 155 · see citing works
Holistic Assessment of Rumen Microbiome Dynamics through Quantitative Metatranscriptomics Reveals Multifunctional Redundancy during Key Steps of Anaerobic Feed Degradation
Söllinger, A., Tveit, A. T., Poulsen, M., Noel, S. J., Bengtsson, M. M., Bernhardt, J., Hellwing, A. L. F., Lund, P., Riedel, K., Schleper, C., et al. (2018) · mSystems · doi:10.1128/msystems.00038-18 · Cited by 152 · see citing works
Lignocellulose degradation by rumen bacterial communities: New insights from metagenome analyses
Gharechahi, J., Vahidi, M. F., Sharifi, G., Ariaeenejad, S., Ding, X., Han, J., & Salekdeh, G. H. (2023) · Environmental Research · doi:10.1016/j.envres.2023.115925 · Cited by 152 · see citing works
A meta-analysis describing the effects of the essential oils blend agolin ruminant on performance, rumen fermentation and methane emissions in dairy cows
Belanche, A., Newbold, C., Morgavi, D., Bach, A., Zweifel, B., & Yáñez-Ruiz, D. (2020) · Animals · doi:10.3390/ani10040620 · Cited by 143 · see citing works
Review: The compositional variation of the rumen microbiome and its effect on host performance and methane emission
Mizrahi, I., & Jami, E. (2018) · animal · doi:10.1017/s1751731118001957 · Cited by 147 · see citing works
Strategies Used to Reduce Methane Emissions from Ruminants: Controversies and Issues
Króliczewska, B., Pecka‐Kiełb, E., & Bujok, J. (2023) · Agriculture · doi:10.3390/agriculture13030602 · Cited by 157 · see citing works
PCR and omics based techniques to study the diversity, ecology and biology of anaerobic fungi: insights, challenges and opportunities
Edwards, J. E., Forster, R. J., Callaghan, T. M., Dollhofer, V., Dagar, S. S., Cheng, Y., Chang, J., Kittelmann, S., Fliegerova, K., Puniya, A. K., et al. (2017) · Frontiers in Microbiology · doi:10.3389/fmicb.2017.01657 · Cited by 130 · see citing works
Essential Biomolecules in Food Webs
Rueß, L., & Müller‐Navarra, D. C. (2019) · Frontiers in Ecology and Evolution · doi:10.3389/fevo.2019.00269 · Cited by 128 · see citing works
Meta-analysis quantifying the potential of dietary additives and rumen modifiers for methane mitigation in ruminant production systems
Almeida, A. K., Hegarty, R. S., & Cowie, A. (2021) · Animal nutrition · doi:10.1016/j.aninu.2021.09.005 · Cited by 128 · see citing works
Invited review: Application of meta-omics to understand the dynamic nature of the rumen microbiome and how it responds to diet in ruminants
Gruninger, R. J., Ribeiro, G. O., Cameron, A., & McAllister, T. A. (2019) · animal · doi:10.1017/s1751731119000752 · Cited by 119 · see citing works
Alternative pathways for hydrogen sink originated from the ruminal fermentation of carbohydrates: Which microorganisms are involved in lowering methane emission?
Pereira, A. M., Dapkevicius, M. D. L. E., & Borba, A. E. S. (2022) · Animal Microbiome · doi:10.1186/s42523-021-00153-w · Cited by 113 · see citing works
RETRACTED: Biotechnological potential of rumen microbiota for sustainable bioconversion of lignocellulosic waste to biofuels and value-added products
Bhujbal, S. K., Ghosh, P., Vijay, V. K., Rathour, R., Kumar, M., Singh, L., & Kapley, A. (2022) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2021.152773 · Cited by 106 · see citing works
Retracted
Enteric Methane Emissions and Animal Performance in Dairy and Beef Cattle Production: Strategies, Opportunities, and Impact of Reducing Emissions
Min, B. R., Lee, S., Jung, H.-J., Miller, D. N., & Chen, R. (2022) · Animals · doi:10.3390/ani12080948 · Cited by 103 · see citing works
Identification of Complex Rumen Microbiome Interaction Within Diverse Functional Niches as Mechanisms Affecting the Variation of Methane Emissions in Bovine
Martínez-Álvaro, M., Auffret, M. D., Stewart, R. D., Dewhurst, R. J., Duthie, C.-A., Rooke, J. A., Wallace, R. J., Shih, B. B.-J., Freeman, T. C., Watson, M., et al. (2020) · Frontiers in Microbiology · doi:10.3389/fmicb.2020.00659 · Cited by 101 · see citing works
Heat Stress: Effects on Rumen Microbes and Host Physiology, and Strategies to Alleviate the Negative Impacts on Lactating Dairy Cows
Kim, S., Ramos, S., Valencia, R. A., Cho, Y.-I., & Lee, S. (2022) · Frontiers in Microbiology · doi:10.3389/fmicb.2022.804562 · Cited by 114 · see citing works
Inhibition of Rumen Methanogenesis and Ruminant Productivity: A Meta-Analysis
Ungerfeld, E. M. (2018) · Frontiers in Veterinary Science · doi:10.3389/fvets.2018.00113 · Cited by 97 · see citing works
Genomic insights into the phylogeny and biomass-degrading enzymes of rumen ciliates
Li, Z., Wang, X., Zhang, Y., Yu, Z., Zhang, T., Dai, X., Pan, X., Jing, R., Yan, Y., Liu, Y., et al. (2022) · The ISME Journal · doi:10.1038/s41396-022-01306-8 · Cited by 94 · see citing works
Illumina sequencing analysis of the ruminal microbiota in high-yield and low-yield lactating dairy cows
Tong, J., Zhang, H., Yang, D., Zhang, Y., Xiong, B., & Jiang, L. (2018) · PLoS ONE · doi:10.1371/journal.pone.0198225 · Cited by 93 · see citing works
Characterization of the ruminal fermentation and microbiome in lambs supplemented with hydrolysable and condensed tannins
Salami, S. A., Valenti, B., Bella, M., O ' Grady, M. N., Luciano, G., Kerry, J. P., Jones, E., Priolo, A., & Newbold, C. J. (2018) · FEMS Microbiology Ecology · doi:10.1093/femsec/fiy061 · Cited by 91 · see citing works
Does intra-ruminal nitrogen recycling waste valuable resources? A review of major players and their manipulation
Hartinger, T., Gresner, N., & Südekum, K. (2018) · Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology · doi:10.1186/s40104-018-0249-x · Cited by 91 · see citing works
A Review of Effect of Saponins on Ruminal Fermentation, Health and Performance of Ruminants
KHOLIF, A. E. (2023) · Veterinary Sciences · doi:10.3390/vetsci10070450 · Cited by 106 · see citing works
Effect of Pre-weaning Diet on the Ruminal Archaeal, Bacterial, and Fungal Communities of Dairy Calves
Dias, J., Marcondes, M. I., Noronha, M. F., Resende, R. T., Machado, F. S., Mantovani, H. C., Dill‐McFarland, K. A., & Suen, G. (2017) · Frontiers in Microbiology · doi:10.3389/fmicb.2017.01553 · Cited by 89 · see citing works
Early-diverging fungal phyla: taxonomy, species concept, ecology, distribution, anthropogenic impact, and novel phylogenetic proposals
Voigt, K., James, T. Y., Kirk, P. M., Santiago, A. L. C. M. D. A., Waldman, B., Griffith, G. W., Fu, M., Radek, R., Strassert, J. F. H., Wurzbacher, C., et al. (2021) · Fungal Diversity · doi:10.1007/s13225-021-00480-y · Cited by 88 · see citing works
Management of Enteric Methane Emissions in Ruminants Using Feed Additives: A Review
Palangı, V., & Lackner, M. K. (2022) · Animals · doi:10.3390/ani12243452 · Cited by 83 · see citing works
Repeated inoculation of cattle rumen with bison rumen contents alters the rumen microbiome and improves nitrogen digestibility in cattle
Ribeiro, G. O., Oss, D. B., He, Z., Gruninger, R. J., Elekwachi, C. O., Forster, R. J., Yang, W. Z., Beauchemin, K. A., & McAllister, T. A. (2017) · Scientific Reports · doi:10.1038/s41598-017-01269-3 · Cited by 80 · see citing works
Defining functional diversity for lignocellulose degradation in a microbial community using multi-omics studies
Alessi, A. M., Bird, S. M., Oates, N. C., Li, Y., Dowle, A., Novotny, E. H., DeAzevedo, E. R., Bennett, J. P., Polikarpov, I., Young, J. P. W., et al. (2018) · Biotechnology for Biofuels · doi:10.1186/s13068-018-1164-2 · Cited by 80 · see citing works
Metabolic influence of core ciliates within the rumen microbiome
Andersen, T. O., Altshuler, I., de León, A. V., Walter, J. M., McGovern, E., Keogh, K. A., Martin, C., Bernard, L., Morgavi, D., Park, T., et al. (2023) · The ISME Journal · doi:10.1038/s41396-023-01407-y · Cited by 84 · see citing works
Effects of tannins and saponins contained in foliage of Gliricidia sepium and pods of Enterolobium cyclocarpum on fermentation, methane emissions and rumen microbial population in crossbred heifers
Molina-Botero, I. C., Arroyave-Jaramillo, J., Valencia-Salazar, S. S., Rosales, R. B., Aguilar-Pérez, C. F., Ayala-Burgos, A. J., Arango, J., & Kú-Vera, J. C. (2019) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2019.01.011 · Cited by 76 · see citing works
Protozoa populations are ecosystem engineers that shape prokaryotic community structure and function of the rumen microbial ecosystem
Solomon, R., Wein, T., Levy, B., Eshed, S., Dror, R., Reiss, V., Zehavi, T., Furman, O., Mizrahi, I., & Jami, E. (2020) · bioRxiv · doi:10.1038/s41396-021-01170-y · Cited by 61 · see citing works
Essential Oils as a Dietary Additive for Small Ruminants: A Meta-Analysis on Performance, Rumen Parameters, Serum Metabolites, and Product Quality
Dorantes-Iturbide, G., Orzuna‐Orzuna, J. F., Lara-Bueno, A., Martínez, G. D. M., Miranda‐Romero, L. A., & Lee-Rangel, H. A. (2022) · Veterinary Sciences · doi:10.3390/vetsci9090475 · Cited by 81 · see citing works
Rumen microbes, enzymes, metabolisms, and application in lignocellulosic waste conversion - A comprehensive review
Liang, J., Zhang, R., Chang, J., Chen, L., Nabi, M., Zhang, H., Zhang, G., & Zhang, P. (2024) · Biotechnology Advances · doi:10.1016/j.biotechadv.2024.108308 · Cited by 75 · see citing works
Effects of the dietary inclusion of babassu oil or buriti oil on lamb performance, meat quality and fatty acid composition
Parente, M. D. O. M., da Rocha, K. S., Bessa, R. J. B., Parente, H. N., Zanine, A. D. M., Machado, N. A. F., Júnior, J. D. B. L., Bezerra, L. R., Landim, A. V., & Alves, S. P. (2019) · Meat Science · doi:10.1016/j.meatsci.2019.107971 · Cited by 71 · see citing works
Inoculation with rumen fluid in early life accelerates the rumen microbial development and favours the weaning process in goats
Palma‐Hidalgo, J. M., Jiménez, E., Popova, M., Morgavi, D. P., Martín-García, A. I., Yáñez-Ruíz, D. R., & Belanche, A. (2021) · Animal Microbiome · doi:10.1186/s42523-021-00073-9 · Cited by 71 · see citing works
Hydrogen and formate production and utilisation in the rumen and the human colon
Kelly, W. J., Mackie, R. I., Attwood, G. T., Janssen, P. H., McAllister, T. A., & Leahy, S. C. (2022) · Animal Microbiome · doi:10.1186/s42523-022-00174-z · Cited by 68 · see citing works
Understanding the Diversity and Roles of the Ruminal Microbiome
Keum, G. B., Pandey, S., Kim, E. S., Doo, H., Kwak, J., Ryu, S., Choi, Y., Kang, J., Kim, S., & Kim, H. B. (2024) · The Journal of Microbiology · doi:10.1007/s12275-024-00121-4 · Cited by 75 · see citing works
Maternal versus artificial rearing shapes the rumen microbiome having minor long‐term physiological implications
Belanche, A., Yáñez-Ruíz, D. R., Detheridge, A. P., Griffith, G. W., Kingston‐Smith, A. H., & Newbold, C. J. (2019) · Environmental Microbiology · doi:10.1111/1462-2920.14801 · Cited by 65 · see citing works
The Profound Influence of Gut Microbiome and Extracellular Vesicles on Animal Health and Disease
Barathan, M., Ng, S. L., Lokanathan, Y., Ng, M. H., & Law, J. X. (2024) · International Journal of Molecular Sciences · doi:10.3390/ijms25074024 · Cited by 64 · see citing works
Application of meta-omics techniques to understand greenhouse gas emissions originating from ruminal metabolism
Wallace, R. J., Snelling, T. J., McCartney, C. A., Tapio, I., & Strozzi, F. (2017) · Genetics Selection Evolution · doi:10.1186/s12711-017-0285-6 · Cited by 63 · see citing works
Rumen Protozoa Play a Significant Role in Fungal Predation and Plant Carbohydrate Breakdown
Williams, C. L., Thomas, B. J., McEwan, N. R., Stevens, P. R., Creevey, C. J., & Huws, S. (2020) · Frontiers in Microbiology · doi:10.3389/fmicb.2020.00720 · Cited by 62 · see citing works
In Pursuit of Understanding the Rumen Microbiome
Sanjorjo, R. A., Tseten, T., Kang, M., Kwon, M., & Kim, S.-W. (2023) · Fermentation · doi:10.3390/fermentation9020114 · Cited by 62 · see citing works
Survey of rumen microbiota of domestic grazing yak during different growth stages revealed novel maturation patterns of four key microbial groups and their dynamic interactions
Guo, W., Zhou, M., Ma, T., Bi, S., Wang, W., Zhang, Y., Huang, X., Guan, L. L., & Long, R. (2020) · Animal Microbiome · doi:10.1186/s42523-020-00042-8 · Cited by 59 · see citing works
Structural equation models to disentangle the biological relationship between microbiota and complex traits: Methane production in dairy cattle as a case of study
Saborío‐Montero, A., Gutiérrez‐Rivas, M., García-Rodríguez, A., Atxaerandio, R., Goiri, I., de Maturana, E. L., Jiménez-Montero, J. A., Alenda, R., & González-Recio, Ó. (2019) · Journal of Animal Breeding and Genetics · doi:10.1111/jbg.12444 · Cited by 61 · see citing works
An Investigation into Rumen Fungal and Protozoal Diversity in Three Rumen Fractions, during High-Fiber or Grain-Induced Sub-Acute Ruminal Acidosis Conditions, with or without Active Dry Yeast Supplementation
Ishaq, S. L., AlZahal, O., Walker, N., & McBride, B. (2017) · Frontiers in Microbiology · doi:10.3389/fmicb.2017.01943 · Cited by 61 · see citing works
“…also appear to directly compete with fungi through enzymatic destruction and predation (Hsu et al., 1991; Gruninger et al., 2014), although a meta-analysis has suggested that rumen defaunation more often causes a reduction in cellulolytic microorganisms, including fungi (Newbold et al., 2015).”
Background
Advanced estimation and mitigation strategies: a cumulative approach to enteric methane abatement from ruminants
Islam, M., & Lee, S.-S. (2019) · Journal of Animal Science and Technology · doi:10.5187/jast.2019.61.3.122 · Cited by 60 · see citing works
Tea saponin reduced methanogenesis in vitro but increased methane yield in lactating dairy cows
Guyader, J., Eugène, M., Doreau, M., Morgavi, D. P., Gérard, C., & Martin, C. (2017) · Journal of Dairy Science · doi:10.3168/jds.2016-11644 · Cited by 59 · see citing works
Inoculation with rumen fluid in early life as a strategy to optimize the weaning process in intensive dairy goat systems
Belanche, A., Palma‐Hidalgo, J. M., Nejjam, I., Jiménez, E., Martín-García, A. I., & Yáñez-Ruíz, D. R. (2020) · Journal of Dairy Science · doi:10.3168/jds.2019-18002 · Cited by 59 · see citing works
Identification of Microbial Genetic Capacities and Potential Mechanisms Within the Rumen Microbiome Explaining Differences in Beef Cattle Feed Efficiency
Auffret, M. D., Stewart, R. D., Dewhurst, R. J., Duthie, C.-A., Watson, M., & Roehe, R. (2020) · Frontiers in Microbiology · doi:10.3389/fmicb.2020.01229 · Cited by 59 · see citing works
Nature’s recyclers: anaerobic microbial communities drive crude biomass deconstruction
Lillington, S. P., Leggieri, P. A., Heom, K. A., & O'Malley, M. A. (2019) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2019.08.015 · Cited by 57 · see citing works
Concepts and Consequences of a Core Gut Microbiota for Animal Growth and Development
Perlman, D., Martínez-Álvaro, M., Moraïs, S., Altshuler, I., Hagen, L. H., Jami, E., Roehe, R., Pope, P. B., & Mizrahi, I. (2021) · Annual Review of Animal Biosciences · doi:10.1146/annurev-animal-013020-020412 · Cited by 56 · see citing works
Niche construction and the transition to herbivory: Phenotype switching and the organization of new nutritional modes
Chiu, L., & Gilbert, S. F. (2020) · Elsevier eBooks · doi:10.1016/b978-0-12-817996-3.00015-3 · Cited by 55 · see citing works
A Meta-Analysis of Essential Oils Use for Beef Cattle Feed: Rumen Fermentation, Blood Metabolites, Meat Quality, Performance and, Environmental and Economic Impact
Orzuna‐Orzuna, J. F., Dorantes-Iturbide, G., Lara-Bueno, A., Miranda‐Romero, L. A., Martínez, G. D. M., & Santiago-Figueroa, I. (2022) · Fermentation · doi:10.3390/fermentation8060254 · Cited by 61 · see citing works
Current perspectives on the short- and long-term effects of conventional dairy calf raising systems: a comparison with the natural environment
Cantor, M. C., Neave, H. W., & Costa, J. H. C. (2019) · Translational Animal Science · doi:10.1093/tas/txy144 · Cited by 55 · see citing works
Nutrient digetibility and production responses of lactating dairy cows when saturated free fatty acid supplements are included in diets: A meta-analysis
Neto, J. M. D. S., de Souza, J., & Lock, A. L. (2021) · Journal of Dairy Science · doi:10.3168/jds.2021-20699 · Cited by 50 · see citing works
Short communication: Signs of host genetic regulation in the microbiome composition in 2 dairy breeds: Holstein and Brown Swiss
González-Recio, Ó., Zubiría, I., García-Rodríguez, A., Hurtado, A., & Atxaerandio, R. (2017) · Journal of Dairy Science · doi:10.3168/jds.2017-13179 · Cited by 50 · see citing works
Extending Burk Dehority’s Perspectives on the Role of Ciliate Protozoa in the Rumen
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Authors: Newbold, C. J., de la Fuente, G., Belanche, A., Ramos-Morales, E., & McEwan, N. R.
Affiliations: Aberystwyth University (GB)
Corresponding author: Newbold, Charles J.
ORCID: Newbold, Charles J.
Belanche, Alejandro
McEwan, Neil R.
Venue: Frontiers in Microbiology
Publisher: Frontiers Media SA
Published: 2015-11-26
Volume: 6
Pages: 1313-1313
ISSN: 1664-302X (ISSN-L)
DOI: 10.3389/fmicb.2015.01313
PMC: PMC4659874
OpenAlex: W2177858583
Cited by: 637
References: 163
Citations by year: 2026: 70 · 2025: 67 · 2024: 71 · 2023: 84 · 2022: 81 · 2021: 65 · 2020: 63 · 2019: 46 · 2018: 45 · 2017: 36 · 2016: 8 · 2015: 1
FWCI: 5.26
Type: journal article
Language: en
Open access: gold
doi.org
Licence: cc-by
Locations: Frontiers in Microbiology (publishedVersion, cc-by)
PubMed Central (publishedVersion, cc-by)
PubMed (publishedVersion)
LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (publishedVersion, cc-by)
RECERCAT (Consorci de Serveis Universitaris de Catalunya) (publishedVersion, cc-by)
UdL Open Repository (University of Lleida) (publishedVersion, cc-by)
Open Access Institutional Repository at Robert Gordon University (Robert Gordon University) (publishedVersion, cc-by)
LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (submittedVersion, cc-by)
DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Open Access Institutional Repository at Robert Gordon University (Robert Gordon University) (submittedVersion, cc-by)
Funders: Biotechnology and Biological Sciences Research Council (BB/J0013/1)
Topics: Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
Physical Sciences › Engineering › Building and Construction › Anaerobic Digestion and Biogas Production
Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
Keywords: rumen ciliate protozoa, rumen microbiota, 18S rDNA phylogeny, Entodinium, methanogenesis, methane mitigation, microbial protein supply, plant extracts, cattle, sheep, goats

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The role of ciliate protozoa in the rumen

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