The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
Autofluorescence of Mycobacteria as a Tool for Detection of Mycobacterium tuberculosis
Open accessAbstract
The diagnosis of tuberculosis in developing countries still relies on direct sputum examination by light microscopy, a method that is easy to perform and that is widely applied. However, because of its poor sensitivity and requirement for significant labor and training, light microscopy examination detects the bacilli in only 45 to 60% of all people whose specimens are culture positive for Mycobacterium tuberculosis . Therefore, new diagnostic methods that would enable the detection of the undiagnosed infected population and allow the early commencement of antituberculosis treatment are needed. In this work, the potential use of mycobacterial cyan autofluorescence for the detection of Mycobacterium tuberculosis was explored. The tubercle bacilli were easily visualized as brilliant fluorescent bacilli by microscopy and were easily tracked ex vivo during macrophage infection. Assays with seeded sputum and a 96-well microplate reader fluorimeter indicated that <10 6 bacilli ml −1 of sputum could be detected. Moreover, the use of microplates allowed the examination of only 200 μl of sputum per sample without a loss of sensitivity. Treatment with heat or decontaminating chemical agents did not interfere with the autofluorescence assay; on the contrary, they improved the level of bacterial detection. Autofluorescence for the detection of bacilli is rapid and easy to perform compared to other methodologies and can be performed with minimal training, making this method suitable for implementation in developing countries.
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“However, some organisms exhibiting spontaneous emission spectra following excitation at specific wavelengths may pose problem in the detection of Mycobacteria. These organisms such as spore-forminglike Bacillus subtilis, nonspore-forming bacteria like Staphylococcus aureus, Nocardia, budding yeast may produce fluorescence [13].”
From Correlation of Various Techniques in Diagnosis of Tuberculous Lymphadenitis on Fine Needle Aspiration Cytology (2013) · cites this paper as [13]
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Cited by
Thakur, B., Mehrotra, R., & Nigam, J. S. (2013) · Pathology Research International · doi:10.1155/2013/824620 · Cited by 41
Metadata
- Authors
- Patiño, S., Alamo, L., Cimino, M., Casart, Y., Bartoli, F., García, M. J., & Salazar, L.
- Affiliations
- Departamento de Biología Estructural, Instituto Venezolano de Investigaciones Científicas, Altos de Pipe, Estado Miranda, Venezuela
- Instituto Venezolano de Investigaciones Científicas (VE)
- Departamento de Medicina Preventiva, Facultad de Medicina, Universidad Autónoma de Madrid, Madrid, Spain
- Corresponding author
- Salazar, Leiria
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2008-10
- Volume
- 46
- Issue
- 10
- Pages
- 3296-3302
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- PMC
- PMC2566091
- OpenAlex
- W2150449836
- Cited by
- 59
- References
- 26
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 2 · 2023: 3 · 2022: 4 · 2021: 4 · 2020: 2 · 2019: 2 · 2018: 8 · 2017: 2 · 2016: 7 · 2015: 6 · 2014: 4 · 2013: 2 · 2012: 5
- FWCI
- 0.85
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- journals.asm.org/non-commercial-tdm-license (tdm)
- Locations
- Journal of Clinical Microbiology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- European Commission
- Universidad Complutense de Madrid
- Topics
- Health Sciences › Medicine › Infectious Diseases › Tuberculosis Research and Epidemiology
- Health Sciences › Medicine › Epidemiology › Mycobacterium research and diagnosis
- Health Sciences › Medicine › Infectious Diseases › Infectious Diseases and Tuberculosis
- Keywords
- Mycobacterium tuberculosis, tuberculosis diagnosis, sputum examination, fluorometric determination, macrophage infection, heat treatment (food and biological processing), decontamination, resource-limited settings
- MeSH terms
- Bacteriological Techniques, Fluorescence, Humans, Microscopy, Fluorescence, Mycobacterium tuberculosis, Sensitivity and Specificity, Tuberculosis
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- CoChat. (2026). Citation check: Autofluorescence of Mycobacteria as a Tool for Detection of Mycobacterium tuberculosis. https://app.cochat.ai/papers/10.1128/jcm.02183-08
- The paper
- Patiño, S., Alamo, L., Cimino, M., Casart, Y., Bartoli, F., García, M. J., & Salazar, L. (2008). Autofluorescence of Mycobacteria as a Tool for Detection of Mycobacterium tuberculosis. Journal of Clinical Microbiology. https://doi.org/10.1128/jcm.02183-08
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
