The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Enzymatic RNA amplification of the enteroviruses
Open accessAbstract
Enteroviruses are among the most common causes of childhood infection. Current diagnostic techniques are often too slow and too insensitive to benefit the patient optimally. This report describes a modified polymerase chain reaction technique by which enteroviral RNA can be amplified, over a few hours, to a level detectable by agarose mini-gel electrophoresis or nucleic acid hybridization or both. Three oligomeric regions of great homology among the enteroviruses were identified and designated as a potential primer pair and probe. With this combination, all 11 of the enterovirus serotypes tested, representing the major subgroups of these pathogens, were successfully amplified and detected. The sensitivity and rapidity of this new assay speak to its potential clinical applicability in the diagnosis of enterovirus infections.
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Cited by
Enteroviral infections of the central nervous system.
Rotbart, H. A. (1995) · Clinical Infectious Diseases · doi:10.1093/clinids/20.4.971 · Cited by 312
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Isolation of epidemic poliovirus from sewage during the 1992–3 type 3 outbreak in the Netherlands
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Viral Myocarditis: From Experimental Models to Diagnosis in Patients
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Metadata
- Authors
- Rotbart, H. A.
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1990-03
- Volume
- 28
- Issue
- 3
- Pages
- 438-442
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- OpenAlex
- W1735834838
- Cited by
- 292
- References
- 1
- FWCI
- 17.25
- Type
- journal article
- Language
- en
- Open access
- green
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