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Cited by 372

The DOI matches its Crossref record; 21 of the 21 references it cites were found in a registry or an index.

Rapid and specific detection of verotoxin genes in Escherichia coli by the polymerase chain reaction

Open access
Pollard, D R, Johnson, W M, Lior, H, Tyler, S D, Rozee, K R·1990·Journal of Clinical Microbiology
DOI 10.1128/jcm.28.3.540-545.1990
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Abstract

A set of four synthetic oligonucleotide probes derived from sequences of the VT1 (Shiga-like toxin I [SLT-I]) and VT2 (SLT-II) genes were used in a polymerase chain reaction (PCR) amplification procedure to detect these genes in some enteric pathogens. A total of 40 verotoxin-producing Escherichia coli strains and 43 isolates of other recognized enteric pathogens were studied. PCR amplification products identifying the VT1 and VT2 gene sequences were observed only in nucleic acid extracted from strains found to be VT positive in traditional tissue culture assays. Template nucleic acid extracted from other gram-negative bacteria was found to be negative with the exception of five isolates of Shigella dysenteriae type 1 in which good amplification with the VT1 probe was observed. The oligonucleotide probes clearly distinguished VT1 and VT2 strains of E. coli and did not give specific amplification with nucleic acid from VTe (a SLT-II variant)-producing E. coli. VT1 or VT2 genes or both were not detected in E. coli K-12 strain C600 or HB101 or in strains known to express other virulence factors, such as enterotoxins, adhesins, hemolysins, or unrelated cytotoxins. The sensitivity of the PCR procedure for detection of both VT1 and VT2 genes was determined to be 1 ng of total nucleic acid. Furthermore, the VT1 gene was easily detected when only 100 pg of nucleic acid was used as the template in the PCR procedure.

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Metadata

Authors
Pollard, D. R., Johnson, W. M., Lior, H., Tyler, S. D., & Rozee, K. R.
Venue
Journal of Clinical Microbiology
Publisher
American Society for Microbiology
Published
1990-03
Volume
28
Issue
3
Pages
540-545
ISSN
  • 0095-1137 (ISSN-L)
  • 1098-660X
DOI
10.1128/jcm.28.3.540-545.1990
OpenAlex
W1932989566
Cited by
372
References
21
FWCI
12.94
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Rapid and specific detection of verotoxin genes in Escherichia coli by the polymerase chain reaction. https://app.cochat.ai/papers/10.1128/jcm.28.3.540-545.1990
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Pollard, D. R., Johnson, W. M., Lior, H., Tyler, S. D., & Rozee, K. R. (1990). Rapid and specific detection of verotoxin genes in Escherichia coli by the polymerase chain reaction. Journal of Clinical Microbiology. https://doi.org/10.1128/jcm.28.3.540-545.1990

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.