The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Detection and identification of mycobacteria by amplification of rRNA
Open accessAbstract
Oligonucleotides specific at a genus, group, or species level were defined by a systematic comparison of small-subunit rRNA sequences from Mycobacterium tuberculosis, M. bovis, M. africanum, M. bovis BCG, M. avium, M. kansasii, M. marinum, M. gastri, M. chelonae, M. smegmatis, M. terrae, M. nonchromogenicum, M. xenopi, M. malmoense, M. szulgai, M. scrofulaceum, M. fortuitum, M. gordonae, M. intracellulare, M. simiae, M. flavescens, M. paratuberculosis, M. sphagni, M. cookii, M. komossense, M. phlei, and M. farcinica. On the basis of the defined oligonucleotides, the polymerase chain reaction technique was explored to develop a sensitive taxon-specific detection system for mycobacteria. By using M. tuberculosis as a model system, fewer than 10 bacteria could be reliably detected by this kind of assay. These results suggest that amplification of rRNA sequences by the polymerase chain reaction may provide a highly sensitive and specific tool for the direct detection of microorganisms without the need for prior cultivation.
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Citations
Cites
Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase
Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., & Erlich, H. A. (1988) · Science · doi:10.1126/science.2448875 · Cited by 17,719
Cited by
Troesch, A., Nguyen, H., Miyada, C. G., Desvarenne, S., Gingeras, T. R., Kaplan, P. M., Cros, P., & Mabilat, C. (1999) · Journal of Clinical Microbiology · doi:10.1128/jcm.37.1.49-55.1999 · Cited by 272
Honoré-Bouakline, S., Vincensini, J. P., Giacuzzo, V., Lagrange, P. H., & Herrmann, J. L. (2003) · Journal of Clinical Microbiology · doi:10.1128/jcm.41.6.2323-2329.2003 · Cited by 168
Detection of Mycobacterium bovis in tissues by polymerase chain reaction
Wards, B. (1995) · Veterinary Microbiology · doi:10.1016/0378-1135(94)00096-f · Cited by 114
Pereira, A. C., Ramos, B., Reis, A. C., & Cunha, M. V. (2020) · Microorganisms · doi:10.3390/microorganisms8091380 · Cited by 101
(2003) · PubMed · Cited by 43
Metadata
- Authors
- Böddinghaus, B., Rogall, T., Flohr, T., Blöcker, H., & Böttger, E. C.
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1990-08
- Volume
- 28
- Issue
- 8
- Pages
- 1751-1759
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- OpenAlex
- W1761387917
- Cited by
- 516
- References
- 1
- FWCI
- 24.14
- Type
- journal article
- Language
- en
- Open access
- green
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- Böddinghaus, B., Rogall, T., Flohr, T., Blöcker, H., & Böttger, E. C. (1990). Detection and identification of mycobacteria by amplification of rRNA. Journal of Clinical Microbiology. https://doi.org/10.1128/jcm.28.8.1751-1759.1990
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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