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Cited by 413

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Insertion element IS986 from Mycobacterium tuberculosis: a useful tool for diagnosis and epidemiology of tuberculosis

Open access
Hermans, P W, van Soolingen, D, Dale, J W, Schuitema, A R, McAdam, R A, Catty, D, van Embden, J D·1990·Journal of Clinical Microbiology

Abstract

IS986 of Mycobacterium tuberculosis belongs to the IS3-like family of insertion sequences, and it has previously been shown to be present in multiple copies in the chromosome of M. tuberculosis. In this study we investigated the value of a IS986-based DNA probe in the diagnosis and epidemiology of tuberculosis. IS986 was found only in species belonging to the M. tuberculosis complex. Independent isolates of M. tuberculosis complex strains showed a very high degree of polymorphism of restriction fragments which contained IS986 DNA. In contrast, Mycobacterium bovis BCG vaccine strains as well as clinical isolates of M. bovis BCG contained one copy of IS986, which was present at the same location in the chromosome. Different M. tuberculosis isolates from a recent M. tuberculosis outbreak showed an identical banding pattern. We concluded that IS986 is an extremely suitable tool for the diagnosis and epidemiology of tuberculosis.

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Crossref · 10.1128/jcm.28.9.2051-2058.1990

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Metadata

Authors
Hermans, P. W., van Soolingen, D., Dale, J. W., Schuitema, A. R., McAdam, R. A., Catty, D., & van Embden, J. D.
Venue
Journal of Clinical Microbiology
Published
1990-09
Volume
28
Issue
9
Pages
2051-2058
ISSN
  • 0095-1137 (ISSN-L)
  • 1098-660X
OpenAlex
W2172204738
Cited by
413
References
1
FWCI
29.72
Type
journal article
Language
en
Open access
green

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The paper
Hermans, P. W., van Soolingen, D., Dale, J. W., Schuitema, A. R., McAdam, R. A., Catty, D., & van Embden, J. D. (1990). Insertion element IS986 from Mycobacterium tuberculosis: a useful tool for diagnosis and epidemiology of tuberculosis. Journal of Clinical Microbiology. https://doi.org/10.1128/jcm.28.9.2051-2058.1990

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.