The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Sensitive detection of Treponema pallidum by using the polymerase chain reaction
Open accessAbstract
We have developed a sensitive assay for Treponema pallidum subsp. pallidum (T. pallidum), the agent of veneral syphilis, based upon the polymerase chain reaction (PCR). A 658-bp portion of the gene encoding the 47-kDa membrane immunogen was amplified, and the PCR products were probed by DNA-DNA hybridization with a 496-bp fragment internal to the amplitifed DNA. The assay detected approximately 0.01 pg of purified T. pallidum DNA, and positive results were obtained routinely from suspensions of treponemes calculated to contain 10 or more organism and from some suspensions calculated to contain a single organism. Specific PCR products were obtained for the closely related agent of yaws, Treponema pallidum subsp. pertenue, but not with human DNA or DNAs from other spirochetes (including Borrelia burgdoferi), skin microorganisms, sexually transmitted disease pathogens, and central nervous system pathogens. T. pallidum DNA was detected in serum, cerebrospinal fluids, and amniotic fluids from syphilis patients but not in in nonsyphilitic controls. T. pallidum DNA was also amplified from paraffin-embedded tissue. The diagnosis of syphillis by using PCR may become a significant addition to the diagnostic armamentarium and a valuable technique for the investigation of syphilis pathogenesis.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase
Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., & Erlich, H. A. (1988) · Science · doi:10.1126/science.2448875 · Cited by 17,719
Lukehart, S. A., Hook, E. W., Baker-Zander, S. A., Collier, A. C., Critchlow, C. W., & Handsfield, H. H. (1988) · Annals of Internal Medicine · doi:10.7326/0003-4819-109-11-855 · Cited by 603 · cited 4×
“While potentially important, these results are inconsistent with published RIT analyses of spinal fluids from late syphilis patients (21, 23) and they were not corroborated by companion RIT data.”
BackgroundResultInfluentialSelf-citation
Cited by
Syphilis: Review with Emphasis on Clinical, Epidemiologic, and Some Biologic Features
Singh, A. E., & Romanowski, B. (1999) · Clinical Microbiology Reviews · doi:10.1128/cmr.12.2.187 · Cited by 638
The Laboratory Diagnosis of Syphilis
Ratnam, S. (2005) · Canadian Journal of Infectious Diseases and Medical Microbiology · doi:10.1155/2005/597580 · Cited by 257
Use of PCR in the diagnosis of early syphilis in the United Kingdom
Palmer, H. M. (2003) · Sexually Transmitted Infections · doi:10.1136/sti.79.6.479 · Cited by 150
Martin, I. E., Gu, W., Yang, Y., & Tsang, R. S. W. (2009) · Clinical Infectious Diseases · doi:10.1086/600878 · Cited by 85
Morshed, M., Lee, M.-K., Laley, J., Cook, D., Mak, A., Chahil, N., Ryan, V., Montgomery, C., Makaroff, S., Malleson, S., et al. (2022) · Microbiology Spectrum · doi:10.1128/spectrum.00686-22 · Cited by 12
Metadata
- Authors
- Burstain, J. M., Grimprel, E., Lukehart, S. A., Norgard, M. V., & Radolf, J. D.
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1991-01
- Volume
- 29
- Issue
- 1
- Pages
- 62-69
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- OpenAlex
- W1641314938
- Cited by
- 212
- References
- 2
- FWCI
- 8.42
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Sensitive detection of Treponema pallidum by using the polymerase chain reaction. https://app.cochat.ai/papers/10.1128/jcm.29.1.62-69.1991
- The paper
- Burstain, J. M., Grimprel, E., Lukehart, S. A., Norgard, M. V., & Radolf, J. D. (1991). Sensitive detection of Treponema pallidum by using the polymerase chain reaction. Journal of Clinical Microbiology. https://doi.org/10.1128/jcm.29.1.62-69.1991
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
