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Cited by 306

The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.

Molecular typing of Staphylococcus aureus on the basis of coagulase gene polymorphisms

Open access
Goh, S H, Byrne, S K, Zhang, J L, Chow, A W·1992·Journal of Clinical Microbiology

Abstract

Staphylocoagulase, a major phenotypic determinant of Staphylococcus aureus, exists in multiple allelic forms, in part because of the existence of gene variants within the 3'-end coding region. This region contains a series of repeating 81-bp DNA sequences which differ both in the number of tandem repeats and the location of AluI restriction sites among different isolates. Utilizing this finding, we developed a novel typing method for S. aureus based on polymerase chain reaction amplification of the variable region of the coagulase gene followed by AluI restriction enzyme digestion and analysis of restriction fragment length polymorphism (RFLP). Among 30 S. aureus isolates studied initially, a total of 10 distinct RFLP patterns were observed. There was excellent correlation of the RFLP patterns with typing of these isolates by multilocus enzyme electrophoresis at 20 chromosomal loci. This coagulase RFLP method was used to analyze an additional 39 S. aureus isolates and successfully traced the source of an outbreak of methicillin-resistant S. aureus infections at a local hospital.

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Crossref · 10.1128/jcm.30.7.1642-1645.1992

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Metadata

Authors
Goh, S. H., Byrne, S. K., Zhang, J. L., & Chow, A. W.
Venue
Journal of Clinical Microbiology
Published
1992-07
Volume
30
Issue
7
Pages
1642-1645
ISSN
  • 0095-1137 (ISSN-L)
  • 1098-660X
OpenAlex
W1904339705
Cited by
306
References
7
FWCI
8.60
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Molecular typing of Staphylococcus aureus on the basis of coagulase gene polymorphisms. https://app.cochat.ai/papers/10.1128/jcm.30.7.1642-1645.1992
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Goh, S. H., Byrne, S. K., Zhang, J. L., & Chow, A. W. (1992). Molecular typing of Staphylococcus aureus on the basis of coagulase gene polymorphisms. Journal of Clinical Microbiology. https://doi.org/10.1128/jcm.30.7.1642-1645.1992

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.