The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Detection of bacterial DNA in cerebrospinal fluid by an assay for simultaneous detection of Neisseria meningitidis, Haemophilus influenzae, and streptococci using a seminested PCR strategy
Open accessAbstract
Primers specific to conserved and variable regions in the 16S rRNA sequence were selected from the partially sequenced 16S rRNA genes of Neisseria meningitidis, Haemophilus influenzae, Streptococcus pneumoniae, S. agalactiae, and Staphylococcus epidermidis. The PCR assay was divided into two DNA amplifications. The first resulted in a general bacterial amplicon, and the second resulted in a species-specific amplicon. The high specificity of the PCR assay was documented after testing bacteria of 28 different species (133 strains). A total of 304 clinical cerebrospinal fluid samples, including 125 samples from patients with bacterial meningitis, were assayed to investigate the diagnostic sensitivity and specificity for bacterial meningitis. The assay showed high sensitivity (0.94) and specificity (0.96) with the clinical samples, although some false results were obtained, the reasons for which are discussed. With agarose gel electrophoresis for detection of the PCR products, the detection limit for meningococci in cerebrospinal fluid was 3 x 10(2) CFU/ml.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase
Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., & Erlich, H. A. (1988) · Science · doi:10.1126/science.2448875 · Cited by 17,719
van Ketel, R. J., de Wever, B., & van Alphen, L. (1990) · Journal of Medical Microbiology · doi:10.1099/00222615-33-4-271 · Cited by 122
Cited by
Practice Guidelines for the Management of Bacterial Meningitis
Tunkel, A. R., Hartman, B. J., Kaplan, S. L., Kaufman, B. A., Roos, K. L., Scheld, W. M., & Whitley, R. J. (2004) · Clinical Infectious Diseases · doi:10.1086/425368 · Cited by 2,308
Effects of Amplification Facilitators on Diagnostic PCR in the Presence of Blood, Feces, and Meat
Abu Al-Soud, W., & Rådström, P. (2000) · Journal of Clinical Microbiology · doi:10.1128/jcm.38.12.4463-4470.2000 · Cited by 398
Simultaneous detection and quantification of three bacterial meningitis pathogens by SERS
Gracie, K., Correa, E., Mabbott, S., Dougan, J. A., Graham, D., Goodacre, R., & Faulds, K. (2014) · Chem. Sci. · doi:10.1039/c3sc52875h · Cited by 161
Chatelet, I. P. D., Traore, Y., Gessner, B. D., Antignac, A., Naccro, B., Njanpop-Lafourcade, B.-M., Ouedraogo, M. S., Tiendrebeogo, S. R., Varon, E., & Taha, M. K. (2005) · Clinical Infectious Diseases · doi:10.1086/426436 · Cited by 158
Welinder-Olsson, C., Dotevall, L., Hogevik, H., Jungnelius, R., Trollfors, B., Wahl, M., & Larsson, P. (2007) · Clinical Microbiology and Infection · doi:10.1111/j.1469-0691.2007.01756.x · Cited by 144
Metadata
- Authors
- Rådström, P., Bäckman, A., Qian, N., Kragsbjerg, P., Påhlson, C., & Olcén, P.
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1994-11
- Volume
- 32
- Issue
- 11
- Pages
- 2738-2744
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- OpenAlex
- W1579906052
- Cited by
- 179
- References
- 2
- FWCI
- 4.87
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Detection of bacterial DNA in cerebrospinal fluid by an assay for simultaneous detection of Neisseria meningitidis, Haemophilus influenzae, and streptococci using a seminested PCR strategy. https://app.cochat.ai/papers/10.1128/jcm.32.11.2738-2744.1994
- The paper
- Rådström, P., Bäckman, A., Qian, N., Kragsbjerg, P., Påhlson, C., & Olcén, P. (1994). Detection of bacterial DNA in cerebrospinal fluid by an assay for simultaneous detection of Neisseria meningitidis, Haemophilus influenzae, and streptococci using a seminested PCR strategy. Journal of Clinical Microbiology. https://doi.org/10.1128/jcm.32.11.2738-2744.1994
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
