The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
PCR amplification and comparison of nucleotide sequences from the groESL heat shock operon of Ehrlichia species
Open accessAbstract
Degenerate PCR primers derived from conserved regions of the eubacterial groESL heat shock operon were used to amplify groESL sequences of Ehrlichia equi, Ehrlichia phagocytophila, the agent of human granulocytic ehrlichiosis (HGE), Ehrlichia canis, Bartonella henselae, and Rickettsia rickettsii. The groESL nucleotide sequences were less conserved than the previously determined 16S rRNA gene sequences of these bacteria. A phylogenetic tree derived from deduced GroEL amino acid sequences was similar to trees based on 16S rRNA gene sequences. Nucleotide sequences obtained from clinical samples containing E. equi, E. phagocytophila, or the HGE agent were very similar (99.9 to 99.0% identity), and the deduced amino acid sequences were identical. Some divergence was evident between nucleotide sequences amplified from samples originating from the United States (E. equi and the HGE agent) and sequences from the European species, E. phagocytophila. A single pair of PCR primers derived from these sequences was used to detect E. chaffeensis and HGE agent DNA in blood samples from human patients with ehrlichiosis.
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Cites
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Identification of a granulocytotropic Ehrlichia species as the etiologic agent of human disease
Chen, S. M., Dumler, J. S., Bakken, J. S., & Walker, D. H. (1994) · Journal of Clinical Microbiology · doi:10.1128/jcm.32.3.589-595.1994 · Cited by 993
Amplification of bacterial 16S ribosomal DNA with polymerase chain reaction
Wilson, K. H., Blitchington, R. B., & Greene, R. C. (1990) · Journal of Clinical Microbiology · doi:10.1128/jcm.28.9.1942-1946.1990 · Cited by 647
Ehrlichia chaffeensis, a new species associated with human ehrlichiosis
Anderson, B. E., Dawson, J. E., Jones, D. C., & Wilson, K. H. (1991) · Journal of Clinical Microbiology · doi:10.1128/jcm.29.12.2838-2842.1991 · Cited by 521
Regnery, R. L., Anderson, B. E., Clarridge, J. E., Rodriguez-Barradas, M. C., Jones, D. C., & Carr, J. H. (1992) · Journal of Clinical Microbiology · doi:10.1128/jcm.30.2.265-274.1992 · Cited by 474
Direct Cultivation of the Causative Agent of Human Granulocytic Ehrlichiosis
Goodman, J. L., Nelson, C., Vitale, B., Madigan, J. E., Dumler, J. S., Kurtti, T. J., & Munderloh, U. G. (1996) · New England Journal of Medicine · doi:10.1056/nejm199601253340401 · Cited by 459
Clinical and Laboratory Characteristics of Human Granulocytic Ehrlichiosis
Bakken, J. S. (1996) · JAMA: The Journal of the American Medical Association · doi:10.1001/jama.1996.03530270039029 · Cited by 451
The tribe Ehrlichieae and ehrlichial diseases
Rikihisa, Y. (1991) · Clinical Microbiology Reviews · doi:10.1128/cmr.4.3.286 · Cited by 451
Isolation and characterization of an Ehrlichia sp. from a patient diagnosed with human ehrlichiosis
Dawson, J. E., Anderson, B. E., Fishbein, D. B., Sanchez, J. L., Goldsmith, C. S., Wilson, K. H., & Duntley, C. W. (1991) · Journal of Clinical Microbiology · doi:10.1128/jcm.29.12.2741-2745.1991 · Cited by 399
Ixodes dammini as a Potential Vector of Human Granulocytic Ehrlichiosis
Pancholi, P., Kolbert, C. P., Mitchell, P. D., Reed, K. D., Dumler, J. S., Bakken, J. S., Telford, S. R., & Persing, D. H. (1995) · Journal of Infectious Diseases · doi:10.1093/infdis/172.4.1007 · Cited by 343
Cited by
Dumler, J. S., Barbet, A. F., Bekker, C. P., Dasch, G. A., Palmer, G. H., Ray, S. C., Rikihisa, Y., & Rurangirwa, F. R. (2001) · International Journal of Systematic and Evolutionary Microbiology · doi:10.1099/00207713-51-6-2145 · Cited by 2,089
Anaplasma spp. in Wild Mammals and Ixodes ricinus from the North of Spain
Portillo, A., Pérez-Martínez, L., Santibáñez, S., Santibáñez, P., Palomar, A. M., & Oteo, J. A. (2011) · Vector-Borne and Zoonotic Diseases · doi:10.1089/vbz.2009.0214 · Cited by 41
Metadata
- Authors
- Sumner, J. W., Nicholson, W. L., & Massung, R. F.
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1997-08
- Volume
- 35
- Issue
- 8
- Pages
- 2087-2092
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- OpenAlex
- W2119694576
- Cited by
- 263
- References
- 25
- FWCI
- 12.25
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: PCR amplification and comparison of nucleotide sequences from the groESL heat shock operon of Ehrlichia species. https://app.cochat.ai/papers/10.1128/jcm.35.8.2087-2092.1997
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- Sumner, J. W., Nicholson, W. L., & Massung, R. F. (1997). PCR amplification and comparison of nucleotide sequences from the groESL heat shock operon of Ehrlichia species. Journal of Clinical Microbiology. https://doi.org/10.1128/jcm.35.8.2087-2092.1997
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
