The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.
Rapid Identification of Candida Species with Species-Specific DNA Probes
Open accessAbstract
Rapid identification of Candida species has become more important because of an increase in infections caused by species other than Candida albicans , including species innately resistant to azole antifungal drugs. We previously developed a PCR assay with an enzyme immunoassay (EIA) format to detect amplicons from the five most common Candida species by using universal fungal primers and species-specific probes directed to the ITS2 region of the gene for rRNA. We designed probes to detect seven additional Candida species ( C. guilliermondii , C. kefyr , C. lambica , C. lusitaniae , C. pelliculosa , C. rugosa , and C. zeylanoides ) included in the API 20C sugar assimilation panel, five probes for species not identified by API 20C ( C. haemulonii , C. norvegica , C. norvegensis , C. utilis , and C. viswanathii ), and a probe for the newly described species C. dubliniensis , creating a panel of 18 Candida species probes. The PCR-EIA correctly identified multiple strains of each species tested, including five identified as C. albicans by the currently available API 20C database but determined to be C. dubliniensis by genotypic and nonroutine phenotypic characteristics. Species identification time was reduced from a mean of 3.5 days by conventional identification methods to 7 h by the PCR-EIA. This method is simple, rapid, and feasible for identifying Candida species in clinical laboratories that utilize molecular identification techniques and provides a novel method to differentiate the new species, C. dubliniensis , from C. albicans .
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“When CDC and submitting laboratory identifications differed, isolate identification was confirmed by use of a DNA probe-based PCR-enzyme immunoassay method (7, 10).”
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Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics
White, T. J., Bruns, T., Lee, S., & Taylor, J. (1990) · PCR Protocols · doi:10.1016/b978-0-12-372180-8.50042-1 · Cited by 36,648
Manual of Clinical Microbiology.
(1980) · Annals of Internal Medicine · doi:10.7326/0003-4819-93-6-939_2 · Cited by 14,435
Avoiding false positives with PCR
Kwok, S., & Higuchi, R. (1989) · Nature · doi:10.1038/339237a0 · Cited by 3,407
Epidemiology of nosocomial fungal infections
Fridkin, S. K., & Jarvis, W. R. (1996) · Clinical Microbiology Reviews · doi:10.1128/cmr.9.4.499 · Cited by 782
Sullivan, D. J., Haynes, K. A., Bennett, D. E., Coleman, D. C., & Westerneng, T. J. (1995) · Microbiology · doi:10.1099/13500872-141-7-1507 · Cited by 763
Epidemiology of Nosocomial Fungal Infections, with Emphasis on Candida Species
Jarvis, W. R. (1995) · Clinical Infectious Diseases · doi:10.1093/clinids/20.6.1526 · Cited by 636
Importance of Candida Species Other than C. albicans as Pathogens in Oncology Patients
Wingard, J. R. (1995) · Clinical Infectious Diseases · doi:10.1093/clinids/20.1.115 · Cited by 542
Nosocomial Candidemia: Risk Factors and Attributable Mortality
Wenzel, R. P. (1995) · Clinical Infectious Diseases · doi:10.1093/clinids/20.6.1531 · Cited by 502
Candida dubliniensis : Characteristics and Identification
Sullivan, D. J., & Coleman, D. C. (1998) · Journal of Clinical Microbiology · doi:10.1128/jcm.36.2.329-334.1998 · Cited by 383
Rex, J. H., Pfaller, M. A., Barry, A. L., Nelson, P. W., & Webb, C. D. (1995) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.39.1.40 · Cited by 300
Research works printed but not linked: 1
- Westerman J. M. Elie C. M. Morrison C. J. Improved identification of Candida species using biotinylated species-specific DNA probes abstr. J107 Abstracts of the 38th Interscience Conference on Antimicrobial Agents and Chemotherapy. 1998 481 American Society for Microbiology Washington D.C
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Hajjeh, R. A., Sofair, A. N., Harrison, L. H., Lyon, G. M., Arthington-Skaggs, B. A., Mirza, S. A., Phelan, M., Morgan, J., Lee-Yang, W., Ciblak, M. A., et al. (2004) · Journal of Clinical Microbiology · doi:10.1128/jcm.42.4.1519-1527.2004 · Cited by 649
“based PCR-enzyme immunoassay method (7, 10).”
Combination antifungal therapy
Johnson, M. D., MacDougall, C., Ostrosky-Zeichner, L., Perfect, J. R., & Rex, J. H. (2004) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.48.3.693-715.2004 · Cited by 541
“Newer technologies can provide nontraditional methods for the estimation of fungal burden levels in human tissues or serum (29, 32, 45, 64, 65, 70, 72, 76, 83, 84, 86, 108, 109, 110, 112, 131, 132, 134, 177, 211, 220, 221), but these assays have generally been developed with the objective of diagnosing disease rather…”
BackgroundRecurrent vulvovaginal candidiasis
Sobel, J. D. (2016) · American Journal of Obstetrics and Gynecology · doi:10.1016/j.ajog.2015.06.067 · Cited by 502
Turenne, C. Y., Sanche, S. E., Hoban, D. J., Karlowsky, J. A., & Kabani, A. M. (1999) · Journal of Clinical Microbiology · doi:10.1128/jcm.37.6.1846-1851.1999 · Cited by 435
“Amplification techniques, with subsequent probing of the amplicons with species-specific probes or in a PCR-enzyme immunoassay format, have been utilized to overcome the problems of sensitivity, specificity, and delay encountered with conventional methodology (4, 5, 7, 10, 22, 24, 29).”
MethodIwen, P. C., Hinrichs, S. H., & Rupp, M. E. (2002) · Medical Mycology · doi:10.1080/mmy.40.1.87.109 · Cited by 380
Metadata
- Authors
- Elie, C. M., Lott, T. J., Reiss, E., & Morrison, C. J.
- Affiliations
- Centers for Disease Control and Prevention (US)
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1998-11
- Volume
- 36
- Issue
- 11
- Pages
- 3260-3265
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- PMC
- PMC105312
- OpenAlex
- W2152603420
- Cited by
- 159
- References
- 30
- Citations by year
- 2025: 2 · 2024: 1 · 2022: 2 · 2021: 2 · 2020: 1 · 2018: 1 · 2017: 6 · 2015: 4 · 2014: 6 · 2013: 2 · 2012: 2
- FWCI
- 6.03
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Clinical Microbiology (publishedVersion)
- PubMed Central (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed (publishedVersion)
- Funders
- U.S. Department of Energy
- Georgia State University
- Centers for Disease Control and Prevention
- Oak Ridge Institute for Science and Education
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Immunology and Microbiology › Microbiology › Yeasts and Rust Fungi Studies
- Health Sciences › Medicine › Microbiology (medical) › Bacterial Identification and Susceptibility Testing
- Keywords
- Candida species identification, species-specific DNA probes, Candida dubliniensis, Candida albicans, non-albicans Candida, azole resistance
- MeSH terms
- Base Sequence, Candida, Candida albicans, Candidiasis, Digoxigenin, Evaluation Studies as Topic, Genotype, Humans, Immunoenzyme Techniques, Mycology, Phenotype, Sensitivity and Specificity, Species Specificity, DNA Probes, Polymerase Chain Reaction
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