Molecular Typing of Environmental and Patient Isolates of Aspergillus fumigatus from Various Hospital Settings
Open accessAbstract
Fingerprinting of more than 700 clinical and environmental isolates of Aspergillus fumigatus from four differential hospital settings was undertaken with a dispersed repeated DNA sequence. The analysis of the environmental isolates showed that the airborne A. fumigatus population is extremely diverse, with 85% of the strains being represented as a single genotype isolated once. The remaining 15% of the strains were isolated several times and were able to persist for several months in the same hospital environment. No strains were found to be associated with a specific location inside the hospital, and identical strains were isolated from different buildings of the hospital and outdoors. Isolation of the same strain both from patients and from the environment of the same hospital is highly suggestive of a nosocomial infection. The characteristics of the environmental fungal population explains the two main results obtained from the typing of the clinical isolates: (i) the absence of a common strain responsible for an invasive aspergillosis outbreak results from the extreme diversity of the environmental population of A. fumigatus in contact with the patients, and (ii) patients hospitalized in different wards of the same hospital can be infected with the same strain since every patient might inhale the same spore population.
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Population genetic studies provide evidence for recombination in A. fumigatus.
“First, evidence for recombination came from population genetic studies (4, 5).”
From Aspergillus fumigatus and Aspergillosis in 2019 (2019) · cites this paper as [4]
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- Authors
- Chazalet, V., Debeaupuis, J.-P., Sarfati, J., Lortholary, J., Ribaud, P., Shah, P., Cornet, M., Thien, H. V., Gluckman, E., Brücker, G., & Latgé, J.-P.
- Affiliations
- Institut Pasteur (FR)
- Assistance Publique – Hôpitaux de Paris (FR)
- Hôpital Saint-Louis (FR)
- University Hospital Frankfurt (DE)
- Hôtel-Dieu de Paris (FR)
- <!--label omitted: 6-->Laboratoire de Microbiologie, Hôpital Trousseau, 75012 Paris,6 France, and
- <!--label omitted: 3-->Unité de Greffe de Moelle Osseuse, Hôpital St Louis, 75010 Paris,3
- <!--label omitted: 2-->Assistance Publique Hôpitaux de Paris, SEHP, 75001 Paris,2
- <!--label omitted: 1-->Laboratoire des Aspergillus, Institut Pasteur, 75015 Paris,1
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1998-06
- Volume
- 36
- Issue
- 6
- Pages
- 1494-1500
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- PMC
- PMC104867
- OpenAlex
- W2146377737
- Cited by
- 192
- References
- 45
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 3 · 2023: 4 · 2022: 5 · 2021: 2 · 2020: 3 · 2019: 2 · 2018: 5 · 2017: 1 · 2016: 2 · 2015: 8 · 2014: 13 · 2013: 4 · 2012: 12
- FWCI
- 6.52
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Clinical Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Health Sciences › Medicine › Infectious Diseases › Infection Control in Healthcare
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Keywords
- Aspergillus fumigatus, molecular typing, environmental isolates, nosocomial infections, invasive aspergillosis, outbreak, hospital wards, strain persistence
- MeSH terms
- Air Microbiology, Aspergillosis, Aspergillus fumigatus, DNA, Fungal, Genotype, Hospitals, Humans, DNA Fingerprinting, Mycological Typing Techniques, Molecular Epidemiology
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