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Cited by 279Vector-borne infectious diseasesBrucella: diagnosis, epidemiology, treatmentSyphilis Diagnosis and Treatment

Sensitive and Specific Serodiagnosis of Lyme Disease by Enzyme-Linked Immunosorbent Assay with a Peptide Based on an Immunodominant Conserved Region of Borrelia burgdorferi VlsE

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Liang, Fang Ting, Steere, Allen C., Marques, Adriana R., Johnson, Barbara J. B., Miller, James N., Philipp, Mario T.·1999·Journal of Clinical Microbiology
DOI 10.1128/jcm.37.12.3990-3996.1999PMC PMC85863
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Abstract

VlsE, the variable surface antigen of Borrelia burgdorferi, contains an immunodominant conserved region named IR 6. In the present study, the diagnostic performance of a peptide enzyme-linked immunosorbent assay (ELISA) based on a 26-mer synthetic peptide (C 6) with the IR 6 sequence was explored. Sensitivity was assessed with serum samples (n= 210) collected from patients with clinically defined Lyme disease at the acute (early localized or early disseminated disease), convalescent, or late disease phase. The sensitivities for acute-, convalescent-, and late-phase specimens were 74% (29 of 39), 85 to 90% (34 of 40 to 35 of 39), and 100% (59 of 59), respectively. Serum specimens from early neuroborreliosis patients were 95% positive (19 of 20), and those from an additional group of patients with posttreatment Lyme disease syndrome yielded a sensitivity of 62% (8 of 13). To assess the specificity of the peptide ELISA, 77 serum samples from patients with other spirochetal or chronic infections, autoimmune diseases, or neurologic diseases and 99 serum specimens from hospitalized patients in an area where Lyme disease is not endemic were examined. Only two potential false positives from the hospitalized patients were found, and the overall specificity was 99% (174 of 176). Precision, which was assessed with a panel of positive and negative serum specimens arranged in blinded duplicates, was 100%. Four serum samples with very high anti-OspA antibody titers obtained from four monkeys given the OspA vaccine did not react with the C 6 peptide. This simple, sensitive, specific, and precise ELISA may contribute to alleviate some of the remaining problems in Lyme disease serodiagnosis. Because of its synthetic peptide base, it will be inexpensive to manufacture. It also will be applicable to serum specimens from OspA-vaccinated subjects.

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Authors
Liang, F. T., Steere, A. C., Marques, A. R., Johnson, B. J. B., Miller, J. N., & Philipp, M. T.
Affiliations
  • Tulane University (US)
  • Tufts University (US)
  • National Institutes of Health (US)
  • Centers for Disease Control and Prevention (US)
  • University of California, Los Angeles (US)
ORCID
Venue
Journal of Clinical Microbiology
Publisher
American Society for Microbiology
Published
1999-12
Volume
37
Issue
12
Pages
3990-3996
ISSN
  • 0095-1137 (ISSN-L)
  • 1098-660X
DOI
10.1128/jcm.37.12.3990-3996.1999
PMC
PMC85863
OpenAlex
W2118763814
Cited by
279
References
41
Citations by year
2025: 7 · 2024: 4 · 2023: 11 · 2022: 5 · 2021: 5 · 2020: 6 · 2019: 6 · 2018: 9 · 2017: 9 · 2016: 15 · 2015: 12 · 2014: 16 · 2013: 8 · 2012: 7
FWCI
9.41
Type
journal article
Language
en
Open access
Licence
journals.asm.org/non-commercial-tdm-license (tdm)
Locations
  • Journal of Clinical Microbiology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institute of Allergy and Infectious Diseases (AI 35027)
  • National Center for Research Resources (P51RR000164, RR00164.)
Topics
  • Life Sciences › Immunology and Microbiology › Parasitology › Vector-borne infectious diseases
  • Health Sciences › Veterinary › Small Animals › Brucella: diagnosis, epidemiology, treatment
  • Health Sciences › Medicine › Physiology › Syphilis Diagnosis and Treatment
Keywords
Lyme disease, Borrelia burgdorferi, VlsE, variable surface antigens, peptide-based ELISA, serological diagnosis, sensitivity and specificity, neuroborreliosis, spirochetal infection
MeSH terms
Animals, Antibodies, Bacterial, Antigens, Bacterial, Antigens, Surface, Bacterial Outer Membrane Proteins, Bacterial Proteins, Bacterial Vaccines, Cross Reactions, Enzyme-Linked Immunosorbent Assay, Humans, Lipoproteins, Lyme Disease, Macaca mulatta, Peptides, Sensitivity and Specificity, Serologic Tests, Borrelia burgdorferi Group, Immunodominant Epitopes

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