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Multiplex PCR for Detection of Genes for Staphylococcus aureus Enterotoxins, Exfoliative Toxins, Toxic Shock Syndrome Toxin 1, and Methicillin Resistance
Open accessAbstract
A multiplex PCR assay for detection of genes for staphylococcal enterotoxins A to E ( entA , entB , entC , entD , and entE ), toxic shock syndrome toxin 1 ( tst ), exfoliative toxins A and B ( etaA and etaB ), and intrinsic methicillin resistance ( mecA ) was developed. Detection of femA was used as an internal positive control. The multiplex PCR assay combined the primers for sea to see and femA in one set and those for eta , etb , tst , mecA , and femA in the other set. Validation of the assay was performed using 176 human isolates of Staphylococcus aureus . This assay offers a very specific, quick, reliable, and inexpensive alternative to conventional PCR assays used in clinical laboratories to identify various staphylococcal toxin genes.
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Cited by
Coagulase-positive Staphylococci and Staphylococcus aureus in food products marketed in Italy
Normanno, G., Firinu, A., Virgilio, S., Mula, G., Dambrosio, A., Poggiu, A., Decastelli, L., Mioni, R., Scuota, S., Bolzoni, G., et al. (2005) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2004.05.008 · Cited by 274
Comparative Genomics of Staphylococcus aureus Musculoskeletal Isolates
Cassat, J. E., Dunman, P. M., McAleese, F., Murphy, E., Projan, S. J., & Smeltzer, M. S. (2005) · Journal of Bacteriology · doi:10.1128/jb.187.2.576-592.2005 · Cited by 112
Metadata
- Authors
- Mehrotra, M., Wang, G., & Johnson, W. M.
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2000-03
- Volume
- 38
- Issue
- 3
- Pages
- 1032-1035
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- OpenAlex
- W2102419473
- Cited by
- 750
- References
- 0
- FWCI
- 2.59
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: Multiplex PCR for Detection of Genes for Staphylococcus aureus Enterotoxins, Exfoliative Toxins, Toxic Shock Syndrome Toxin 1, and Methicillin Resistance. https://app.cochat.ai/papers/10.1128/jcm.38.3.1032-1035.2000
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- Mehrotra, M., Wang, G., & Johnson, W. M. (2000). Multiplex PCR for Detection of Genes for Staphylococcus aureus Enterotoxins, Exfoliative Toxins, Toxic Shock Syndrome Toxin 1, and Methicillin Resistance. Journal of Clinical Microbiology. https://doi.org/10.1128/jcm.38.3.1032-1035.2000
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