The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.
Standardization of Immunoglobulin M Capture Enzyme-Linked Immunosorbent Assays for Routine Diagnosis of Arboviral Infections
Open accessAbstract
Immunoglobulin M antibody-capture enzyme-linked immunosorbent assay (MAC-ELISA) is a rapid and versatile diagnostic method that readily permits the combination of multiple assays. Test consolidation is especially important for arthropod-borne viruses (arboviruses) which belong to at least three virus families: the Togaviridae,Flaviviridae, and Bunyaviridae. Using prototype viruses from each of these families and a panel of well-characterized human sera, we have evaluated and standardized a combined MAC-ELISA capable of identifying virus infections caused by members of each virus family. Furthermore, by grouping antigens geographically and utilizing known serological cross-reactivities, we have reduced the number of antigens necessary for testing, while maintaining adequate detection sensitivity. We have determined that a 1:400 serum dilution is most appropriate for screening antiviral antibody, using a positive-to-negative ratio of ≥2.0 as a positive cutoff value. With a blind-coded human serum panel, this combined MAC-ELISA was shown to have test sensitivity and specificity that correlated well with those of other serological techniques.
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Cites
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Self-citationIdentification of Highlands J Virus from a Florida Horse
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Musso, D., & Gubler, D. J. (2016) · Clinical Microbiology Reviews · doi:10.1128/cmr.00072-15 · Cited by 1,469
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Self-citationNeurologic Manifestations and Outcome of West Nile Virus Infection
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Metadata
- Authors
- Martin, D. A., Muth, D. A., Brown, T., Johnson, A. J., Karabatsos, N., & Roehrig, J. T.
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2000-05
- Volume
- 38
- Issue
- 5
- Pages
- 1823-1826
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- OpenAlex
- W1906136187
- Cited by
- 477
- References
- 8
- FWCI
- 18.39
- Type
- journal article
- Language
- en
- Open access
- green
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- Martin, D. A., Muth, D. A., Brown, T., Johnson, A. J., Karabatsos, N., & Roehrig, J. T. (2000). Standardization of Immunoglobulin M Capture Enzyme-Linked Immunosorbent Assays for Routine Diagnosis of Arboviral Infections. Journal of Clinical Microbiology. https://doi.org/10.1128/jcm.38.5.1823-1826.2000
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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