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Quantification of Bacteria Adherent to Gastrointestinal Mucosa by Real-Time PCR
Open accessAbstract
The use of real-time quantitative PCR (5′ nuclease PCR assay) as a tool to study the gastrointestinal microflora that adheres to the colonic mucosa was evaluated. We developed primers and probes based on the 16S ribosomal DNA gene sequences for the detection of Escherichia coli and Bacteroides vulgatus . DNA was isolated from pure cultures and from gut biopsy specimens and quantified by the 5′ nuclease PCR assay. The assay showed a very high sensitivity: as little as 1 CFU of E. coli and 9 CFU of B. vulgatus could be detected. The specificities of the primer-probe combinations were evaluated with samples that were spiked with the species most closely related to E. coli and B. vulgatus and with eight other gut microflora species. Mucosal samples spiked with known amounts of E. coli or B. vulgatus DNA showed no PCR inhibition. We conclude that the 5′ nuclease PCR assay may be a useful alternative to conventional culture techniques to study the actual in vivo composition of a complex microbial community like the gut microflora.
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Research works printed but not linked: 23
- 10.1093/nar/25.17.3389 · DOI · Paper page
- 10.1128/mr.59.1.143-169.1995 · doi:10.1128/mr.59.1.143-169.1995 · Paper page
- 10.1128/JCM.38.5.1747-1752.2000 · DOI · Paper page
- Dunn, D. 1990. Autochthonous microflora of the gastrointestinal tract. Perspect. Colon Rectal Surg.2:105.
- 10.1099/0022-1317-50-8-667 · DOI
- 10.1128/AEM.64.11.4581-4587.1998 · DOI · Paper page
- Finegold S. M. V. L. Sutter and G. E. Mathisen. 1983. Normal indigenous intestinal flora p. 3-31. In D. J. Hentgens (ed.) Human intestinal microflora in health and disease. Academic Press London United Kingdom. · DOI · Paper page
- 10.1128/AEM.64.9.3336-3345.1998 · DOI · Paper page
- 10.1016/S0016-5085(19)34121-6 · DOI · Paper page
- 10.1101/gr.6.10.986 · DOI · Paper page
- 10.1016/S0167-7012(99)00049-4 · DOI · Paper page
- 10.1128/JCM.38.2.814-817.2000 · DOI
- 10.1128/aem.61.8.3069-3075.1995 · DOI · Paper page
- 10.1093/ajcn/27.12.1450 · DOI
- 10.1023/A:1000669317571 · DOI · Paper page
- O'Sullivan D. J. 1999. Methods of analysis of the intestinal microflora p. 23-44. In G. W. Tannock (ed.) Probiotics: a critical review. Horizon Scientific Press Wymondham United Kingdom.
- 10.1007/BF01295996 · DOI · Paper page
- 10.1016/S0167-7012(99)00029-9 · DOI · Paper page
- 10.1128/AEM.65.11.4799-4807.1999 · DOI · Paper page
- 10.1023/A:1002038308506 · DOI
- Welling, G. W., P. Elfferich, G. C. Raangs, A. C. Wildeboer-Veloo, G. J. Jansen, and J. E. Degener. 1997. 16S ribosomal RNA-targeted oligonucleotide probes for monitoring of intestinal tract bacteria. Scand. J. Gastroenterol. Suppl.222:17-19.
- 10.1128/aem.62.7.2273-2278.1996 · DOI · Paper page
- 10.1128/AEM.64.10.3854-3859.1998 · DOI · Paper page
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Metadata
- Authors
- Huijsdens, X. W., Linskens, R. K., Mak, M., Meuwissen, S. G. M., Vandenbroucke-Grauls, C. M. J. E., & Savelkoul, P. H. M.
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2002-12
- Volume
- 40
- Issue
- 12
- Pages
- 4423-4427
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- OpenAlex
- W2143566426
- Cited by
- 350
- References
- 0
- FWCI
- 4.32
- Type
- journal article
- Language
- en
- Open access
- green
- Licence
- journals.asm.org/non-commercial-tdm-license (tdm)
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- Huijsdens, X. W., Linskens, R. K., Mak, M., Meuwissen, S. G. M., Vandenbroucke-Grauls, C. M. J. E., & Savelkoul, P. H. M. (2002). Quantification of Bacteria Adherent to Gastrointestinal Mucosa by Real-Time PCR. Journal of Clinical Microbiology. https://doi.org/10.1128/jcm.40.12.4423-4427.2002
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