The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.
Sequential Multiplex PCR Approach for Determining Capsular Serotypes of Streptococcus pneumoniae Isolates
Open accessAbstract
Accurate serotyping is essential to monitor the changes in the seroepidemiology of Streptococcus pneumoniae . We devised a simple and schematic sequence-based system of seven multiplex PCRs, in a sequence order based upon Active Bacterial Core surveillance (ABCs) serotype distribution during 2002 to 2003, to reliably deduce specific pneumococcal serotypes. A total of 421 isolates from ABCs were randomly chosen to evaluate this system. Two hundred twenty-nine of the isolates (54.3%) were specifically assigned 1 of 17 serotypes by the multiplex PCR system, with the results in complete concordance with conventional serotyping. One hundred seventy-two additional isolates (40.9%) were assigned to 11 specific sets of 2 to 4 serotypes that with one exception (serotypes 6A and 6B) consisted of the single frequently occurring targeted serotype and 1 to 3 additional rare serotypes primarily within the same serogroup as the targeted serotype. Only 20 isolates (4.8%) could not be assigned specific serotypes or serotype sets, since they were either of rare serotypes not included in the assay design or were nonserotypeable. Overall, we found this system to be highly reliable, with the potential to greatly reduce our reliance upon conventional serotyping. Especially important is the capability of this system to give serotype-determining potential to any facility that lacks the expensive typing sera and expertise needed for conventional serotyping yet has the modest equipment necessary for DNA amplification and electrophoresis.
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Multiplex PCR: Critical Parameters and Step-by-Step Protocol
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ADVISORY COMMITTEE ON IMMUNIZATION PRACTICES
Abramson, J. S., Pickering, L., Gilsdorf, J. R., Dunne, E. M., Lawson, H. W., Dubin, G. O., Barr, E., Chesson, H. W., Garnett, G. P., & Markowitz, L. E. (2006) · Cited by 932
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Six newly recognized types of Streptococcus pneumoniae
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Pneumococcal Capsules and Their Types: Past, Present, and Future
Geno, K. A., Gilbert, G. L., Song, J. Y., Skovsted, I. C., Klugman, K. P., Jones, C., Konradsen, H. B., & Nahm, M. H. (2015) · Clinical Microbiology Reviews · doi:10.1128/cmr.00024-15 · Cited by 732
Carvalho, M. D. G., Pimenta, F. C., Jackson, D., Roundtree, A., Ahmad, Y., Millar, E. V., O'Brien, K. L., Whitney, C. G., Cohen, A. L., & Beall, B. W. (2010) · Journal of Clinical Microbiology · doi:10.1128/jcm.02243-09 · Cited by 263
Self-citationVu, H. T. T., Yoshida, L. M., Suzuki, M., Nguyen, H. A. T., Nguyen, C. D. L., Nguyen, A. T. T., Oishi, K., Yamamoto, T., Watanabe, K., Vu, T. D., et al. (2011) · Pediatric Infectious Disease Journal · doi:10.1097/inf.0b013e3181f111a2 · Cited by 208
DE LASTOURS, V., MALOSH, R., RAMADUGU, K., SRINIVASAN, U., DAWID, S., OHMIT, S., & FOXMAN, B. (2016) · Epidemiology and Infection · doi:10.1017/s0950268816001473 · Cited by 20
Hasanuzzaman, M., Saha, S., Malaker, R., Rahman, H., Sajib, M. S. I., Das, R. C., Islam, M., Hamer, D. H., Darmstadt, G. L., & Saha, S. K. (2021) · The Journal of Infectious Diseases · doi:10.1093/infdis/jiab073 · Cited by 15
Metadata
- Authors
- Pai, R., Gertz, R. E., & Beall, B.
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2006-01
- Volume
- 44
- Issue
- 1
- Pages
- 124-131
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- OpenAlex
- W2117383615
- Cited by
- 543
- References
- 24
- FWCI
- 7.75
- Type
- journal article
- Language
- en
- Open access
- green
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