The DOI matches its Crossref record; 16 of the 16 references it cites were found in a registry or an index.
Revisiting Pneumococcal Carriage by Use of Broth Enrichment and PCR Techniques for Enhanced Detection of Carriage and Serotypes
Open accessAbstract
The measurement of pneumococcal carriage in the nasopharyngeal reservoir is subject to potential confounders that include low-density and multiple-strain colonization. To compare different methodologies, we picked a random sampling of 100 nasopharyngeal specimens recovered from infants less than 2 years of age who were previously assessed for pneumococcal carriage and serotypes by a conventional method that used direct plating from the transport/storage medium (50 specimens were culture negative and 50 specimens were culture positive for pneumococci). We used a broth enrichment approach and a conventional PCR approach (with and without broth enrichment) to determine pneumococcal carriage and serotypes, and the results were compared to the initial conventional culture-based results. Additionally, we used a lytA -targeted real-time PCR for pneumococcal detection. Broth enrichment for both the culture-based and the PCR-based methods enhanced the isolation of pneumococci and detection of serotype diversity, with the most effective serotype deduction method being one that used broth enrichment prior to sequential multiplex PCR. Similarly, we also found that broth enrichment followed by the lytA -specific real-time PCR was the most sensitive for the detection of apparent pneumococcal carriage. The broth enrichment, conventional multiplex PCR, and real-time PCR approaches used in this study were effective in detecting pneumococcal carriage in the 50 specimens that were negative by conventional direct plating from transport medium (range of numbers of positive specimens, 8/50 to 22/50 [16 to 44%]), and the three different serotyping approaches that used broth enrichment increased the number of serotype identifications from the 100 specimens (12 to 29 additional serotype identifications to be positive). A PCR-based approach that employed a broth enrichment step appeared to best enhance the detection of mixed serotypes and low-density pneumococcal carriage.
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Gray, B. M., Converse, G. M., & Dillon, H. C. (1980) · Journal of Infectious Diseases · doi:10.1093/infdis/142.6.923 · Cited by 796
Carvalho, M. D. G. S., Tondella, M. L., McCaustland, K., Weidlich, L., McGee, L., Mayer, L. W., Steigerwalt, A., Whaley, M., Facklam, R. R., Fields, B., et al. (2007) · Journal of Clinical Microbiology · doi:10.1128/jcm.02498-06 · Cited by 588
Self-citationPai, R., Gertz, R. E., & Beall, B. (2006) · Journal of Clinical Microbiology · doi:10.1128/jcm.44.1.124-131.2006 · Cited by 543
Self-citationDiscovery of a New Capsular Serotype (6C) within Serogroup 6 of Streptococcus pneumoniae
Park, I. H., Pritchard, D. G., Cartee, R., Brandao, A., Brandileone, M. C. C., & Nahm, M. H. (2007) · Journal of Clinical Microbiology · doi:10.1128/jcm.02199-06 · Cited by 428
O'Brien, K. L., Bronsdon, M. A., Dagan, R., Yagupsky, P., Janco, J., Elliott, J., Whitney, C. G., Yang, Y.-H., Robinson, L.-G. E., Schwartz, B., et al. (2001) · Journal of Clinical Microbiology · doi:10.1128/jcm.39.3.1021-1024.2001 · Cited by 199
Self-citationvan Gils, E. J. M., Veenhoven, R. H., Hak, E., Rodenburg, G. D., Bogaert, D., IJzerman, E. P. F., Bruin, J. P., van Alphen, L., & Sanders, E. A. M. (2009) · JAMA · doi:10.1001/jama.2009.975 · Cited by 161
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Carvalho, M. D. G., Pimenta, F. C., Gertz, R. E., et al. (2008) · Journal of Clinical Microbiology · doi:10.1128/jcm.01919-08 · Cited by 118
Self-citationDias, C. A. G., Teixeira, L. M., Carvalho, M. D. G., et al. (2007) · Journal of Medical Microbiology · doi:10.1099/jmm.0.47347-0 · Cited by 112
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Cited by
Pneumococcal Capsules and Their Types: Past, Present, and Future
Geno, K. A., Gilbert, G. L., Song, J. Y., Skovsted, I. C., Klugman, K. P., Jones, C., Konradsen, H. B., & Nahm, M. H. (2015) · Clinical Microbiology Reviews · doi:10.1128/cmr.00024-15 · Cited by 732
Immunosenescence and pneumococcal disease: an imbalance in host-pathogen interactions.
Krone, C. L., van de Groep, K., Trzciński, K., Sanders, E. A. M., & Bogaert, D. (2014) · The Lancet Respiratory Medicine · doi:10.1016/s2213-2600(13)70165-6 · Cited by 134
Wang, X., Mair, R., Hatcher, C., Theodore, M. J., Edmond, K., Wu, H. M., Harcourt, B. H., Carvalho, M. D. G. S., Pimenta, F., Nymadawa, P., et al. (2011) · International Journal of Medical Microbiology · doi:10.1016/j.ijmm.2010.11.004 · Cited by 120
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Metadata
- Authors
- Carvalho, M. D. G., Pimenta, F. C., Jackson, D., Roundtree, A., Ahmad, Y., Millar, E. V., O'Brien, K. L., Whitney, C. G., Cohen, A. L., & Beall, B. W.
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2010-05
- Volume
- 48
- Issue
- 5
- Pages
- 1611-1618
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- OpenAlex
- W2147711865
- Cited by
- 263
- References
- 16
- FWCI
- 11.11
- Type
- journal article
- Language
- en
- Open access
- green
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- Carvalho, M. D. G., Pimenta, F. C., Jackson, D., Roundtree, A., Ahmad, Y., Millar, E. V., O'Brien, K. L., Whitney, C. G., Cohen, A. L., & Beall, B. W. (2010). Revisiting Pneumococcal Carriage by Use of Broth Enrichment and PCR Techniques for Enhanced Detection of Carriage and Serotypes. Journal of Clinical Microbiology. https://doi.org/10.1128/jcm.02243-09
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