The DOI matches its Crossref record; 55 of the 55 references it cites were found in a registry or an index.
Antigenic and Immunogenic Characterization of Recombinant Baculovirus-Expressed Severe Acute Respiratory Syndrome Coronavirus Spike Protein: Implication for Vaccine Design
Open accessAbstract
The spike (S) glycoprotein of severe acute respiratory syndrome coronavirus (SARS-CoV) mediates the receptor interaction and immune recognition and is considered a major target for vaccine design. However, its antigenic and immunogenic properties remain to be elucidated. In this study, we immunized mice with full-length S protein (FL-S) or its extracellular domain (EC-S) expressed by recombinant baculoviruses in insect cells. We found that the immunized mice developed high titers of anti-S antibodies with potent neutralizing activities against SARS pseudoviruses constructed with the S proteins of Tor2, GD03T13, and SZ3, the representative strains of 2002 to 2003 and 2003 to 2004 human SARS-CoV and palm civet SARS-CoV, respectively. These data suggest that the recombinant baculovirus-expressed S protein vaccines possess excellent immunogenicity, thereby inducing highly potent neutralizing responses against human and animal SARS-CoV variants. The antigenic structure of the S protein was characterized by a panel of 38 monoclonal antibodies (MAbs) isolated from the immunized mice. The epitopes of most anti-S MAbs (32 of 38) were localized within the S1 domain, and those of the remaining 6 MAbs were mapped to the S2 domain. Among the anti-S1 MAbs, 17 MAbs targeted the N-terminal region (amino acids [aa] 12 to 327), 9 MAbs recognized the receptor-binding domain (RBD; aa 318 to 510), and 6 MAbs reacted with the C-terminal region of S1 domain that contains the major immunodominant si…
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“Earlier work had suggested that the spike protein of the coronavirus responsible for the 2002 SARS outbreak was a suitable target for protective immunity. 10”
From Efficacy and Safety of the mRNA-1273 SARS-CoV-2 Vaccine (2021) · cites this paper as [10]
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Drosten, C., Günther, S., Preiser, W., van der Werf, S., Brodt, H.-R., Becker, S., Rabenau, H., Panning, M., Kolesnikova, L., Fouchier, R. A. M., et al. (2003) · New England Journal of Medicine · doi:10.1056/nejmoa030747 · Cited by 4,574
A Novel Coronavirus Associated with Severe Acute Respiratory Syndrome
Tg, K., Erdman, D. D., Cs, G., Zaki, Peret, T. C. T., Emery, S. L., Tong, S., Urbani, C., Comer, J. A., Lim, W., et al. (2003) · New England Journal of Medicine · doi:10.1056/nejmoa030781 · Cited by 4,353
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Peiris, M. J. S., Lai, S., Poon, L. L. M., Guan, Y., Yam, L., Lim, W., Nicholls, J. M., Yee, W., Yan, W., Cheung, M., et al. (2003) · The Lancet · doi:10.1016/s0140-6736(03)13077-2 · Cited by 2,916
Blacklow, N. R., Gorbach, S. L., & Bartlett, J. G. (1992) · Cited by 2,753
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Guan, Y., Zheng, B., He, Y., Liu, X. L., Zhuang, Z. X., Cheung, C. L., Luo, S., Li, P. H., Zhang, L., Guan, Y., et al. (2003) · Science · doi:10.1126/science.1087139 · Cited by 2,404
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- 10.3201/eid1107.050219 · DOI
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Self-citationThe spike protein of SARS-CoV — a target for vaccine and therapeutic development
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Self-citationFrom SARS to MERS, Thrusting Coronaviruses into the Spotlight
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Metadata
- Authors
- He, Y., Li, J., Heck, S., Lustigman, S., & Jiang, S.
- Affiliations
- New York Blood Center (US)
- Corresponding author
- He, Yuxian
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 2006-06-15
- Volume
- 80
- Issue
- 12
- Pages
- 5757-5767
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- OpenAlex
- W2118619855
- Cited by
- 135
- References
- 55
- Citations by year
- 2026: 1 · 2024: 1 · 2023: 8 · 2022: 13 · 2021: 31 · 2020: 35 · 2019: 4 · 2018: 2 · 2017: 1 · 2015: 2 · 2014: 3 · 2013: 1 · 2012: 7
- FWCI
- 3.30
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Journal of Virology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › SARS-CoV-2 and COVID-19 Research
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Virus Infections Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › vaccines and immunoinformatics approaches
- Keywords
- SARS-CoV, spike protein, neutralizing antibodies, receptor binding domain, epitope mapping, monoclonal antibodies, conformational epitopes, S1 domain, S2 domain, antigen characterization, immunogenicity
- MeSH terms
- Animals, Antibodies, Monoclonal, Antibodies, Viral, Antibody Formation, Antigens, Viral, Cloning, Molecular, Humans, Immunization, Membrane Glycoproteins, Recombinant Proteins, Viral Envelope Proteins, Viral Vaccines, Baculoviridae, Epitope Mapping, Severe acute respiratory syndrome-related coronavirus, Mice, Spike Glycoprotein, Coronavirus
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