The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Evidence that Ecotropic Murine Leukemia Virus Contamination in TZM-bl Cells Does Not Affect the Outcome of Neutralizing Antibody Assays with Human Immunodeficiency Virus Type 1
Open accessAbstract
The TZM-bl cell line that is commonly used to assess neutralizing antibodies against human immunodeficiency virus type 1 (HIV-1) was recently reported to be contaminated with an ecotropic murine leukemia virus (MLV) (Y. Takeuchi, M. O. McClure, and M. Pizzato, J. Virol. 82:12585-12588, 2008), raising questions about the validity of results obtained with this cell line. Here we confirm this observation and show that HIV-1 neutralization assays performed with a variety of serologic reagents in a similar cell line that does not harbor MLV yield results that are equivalent to those obtained in TZM-bl cells. We conclude that MLV contamination has no measurable effect on HIV-1 neutralization when TZM-bl cells are used as targets for infection.
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Wei, X., Decker, J. M., Liu, H., Zhang, Z., Arani, R. B., Kilby, J. M., Saag, M. S., Wu, X., Shaw, G. M., & Kappes, J. C. (2002) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.46.6.1896-1905.2002 · Cited by 1,569
Cited by
Sarzotti-Kelsoe, M., Bailer, R. T., Turk, E., Lin, C.-L., Bilska, M., Greene, K. M., Gao, H., Todd, C. A., Ozaki, D. A., Seaman, M. S., et al. (2014) · Journal of Immunological Methods · doi:10.1016/j.jim.2013.11.022 · Cited by 532
Self-citationGardner, M. R., Fetzer, I., Kattenhorn, L. M., Davis-Gardner, M. E., Zhou, A. S., Alfant, B., Weber, J. A., Kondur, H. R., Martinez-Navio, J. M., Fuchs, S. P., et al. (2019) · Molecular Therapy · doi:10.1016/j.ymthe.2019.01.004 · Cited by 60
ISG15 deficiency restricts HIV-1 infection
Jurczyszak, D., Manganaro, L., Buta, S., Gruber, C., Martin-Fernandez, M., Taft, J., Patel, R. S., Cipolla, M., Alshammary, H., Mulder, L. C. F., et al. (2022) · PLOS Pathogens · doi:10.1371/journal.ppat.1010405 · Cited by 32
EWI-2 Inhibits Cell–Cell Fusion at the HIV-1 Virological Presynapse
Whitaker, E. E., Matheson, N. J., Perlee, S., Munson, P. B., Symeonides, M., & Thali, M. (2019) · Viruses · doi:10.3390/v11121082 · Cited by 27
Metadata
- Authors
- Platt, E. J., Bilska, M., Kozak, S. L., Kabat, D., & Montefiori, D. C.
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 2009-08-15
- Volume
- 83
- Issue
- 16
- Pages
- 8289-8292
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- OpenAlex
- W2134950373
- Cited by
- 233
- References
- 1
- FWCI
- 1.57
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Evidence that Ecotropic Murine Leukemia Virus Contamination in TZM-bl Cells Does Not Affect the Outcome of Neutralizing Antibody Assays with Human Immunodeficiency Virus Type 1. https://app.cochat.ai/papers/10.1128/jvi.00709-09
- The paper
- Platt, E. J., Bilska, M., Kozak, S. L., Kabat, D., & Montefiori, D. C. (2009). Evidence that Ecotropic Murine Leukemia Virus Contamination in TZM-bl Cells Does Not Affect the Outcome of Neutralizing Antibody Assays with Human Immunodeficiency Virus Type 1. Journal of Virology. https://doi.org/10.1128/jvi.00709-09
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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