The DOI matches its Crossref record; 21 of the 21 references it cites were found in a registry or an index.
Optimization and validation of the TZM-bl assay for standardized assessments of neutralizing antibodies against HIV-1
Open accessAbstract
The TZM-bl assay measures antibody-mediated neutralization of HIV-1 as a function of reductions in HIV-1 Tat-regulated firefly luciferase (Luc) reporter gene expression after a single round of infection with Env-pseudotyped viruses. This assay has become the main endpoint neutralization assay used for the assessment of preclinical and clinical trial samples by a growing number of laboratories worldwide. Here we present the results of the formal optimization and validation of the TZM-bl assay, performed in compliance with Good Clinical Laboratory Practice (GCLP) guidelines. The assay was evaluated for specificity, accuracy, precision, limits of detection and quantitation, linearity, range and robustness. The validated manual TZM-bl assay was also adapted, optimized and qualified to an automated 384-well format.
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Self-citationMeasuring HIV Neutralization in a Luciferase Reporter Gene Assay
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Self-citationCombination therapy with anti-HIV-1 antibodies maintains viral suppression
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Self-citationBroad and potent activity against SARS-like viruses by an engineered human monoclonal antibody
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High-Throughput Mapping of B Cell Receptor Sequences to Antigen Specificity
Setliff, I., Shiakolas, A. R., Pilewski, K. A., Murji, A. A., Mapengo, R. E., Janowska, K., Richardson, S., Oosthuysen, C., Raju, N., Ronsard, L., et al. (2019) · Cell · doi:10.1016/j.cell.2019.11.003 · Cited by 314
Song, G., He, W.-T., Callaghan, S., Anzanello, F., Huang, D., Ricketts, J., Torres, J. L., Beutler, N., Peng, L., Vargas, S., et al. (2021) · Nature Communications · doi:10.1038/s41467-021-23074-3 · Cited by 285
Metadata
- Authors
- Sarzotti-Kelsoe, M., Bailer, R. T., Turk, E., Lin, C.-L., Bilska, M., Greene, K. M., Gao, H., Todd, C. A., Ozaki, D. A., Seaman, M. S., Mascola, J. R., & Montefiori, D. C.
- Venue
- Journal of Immunological Methods
- Publisher
- Elsevier BV
- Published
- 2014-07
- Volume
- 409
- Pages
- 131-146
- ISSN
- 0022-1759 (ISSN-L)
- OpenAlex
- W2082208556
- Cited by
- 532
- References
- 21
- FWCI
- 7.61
- Type
- journal article
- Language
- en
- Open access
- green
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- Sarzotti-Kelsoe, M., Bailer, R. T., Turk, E., Lin, C.-L., Bilska, M., Greene, K. M., Gao, H., Todd, C. A., Ozaki, D. A., Seaman, M. S., Mascola, J. R., & Montefiori, D. C. (2014). Optimization and validation of the TZM-bl assay for standardized assessments of neutralizing antibodies against HIV-1. Journal of Immunological Methods. https://doi.org/10.1016/j.jim.2013.11.022
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