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Immortalization of human foreskin keratinocytes by various human papillomavirus DNAs corresponds to their association with cervical carcinoma
Abstract
Normal human foreskin keratinocytes cotransfected with the neomycin resistance gene and recombinant human papillomavirus (HPV) DNAs (types 16, 18, 31, and 33) that have a high or moderate association with cervical malignancy acquired immortality and contained integrated and transcriptionally active viral genomes. Only transcripts from the intact E6 and E7 genes were detected in at least one cell line, suggesting that one or both of these genes are responsible for immortalization. Recombinant HPV DNAs with low or no oncogenic potential for cervical cancer (HPV1a, -5, -6b, and -11) induced small G418-resistant colonies that senesced as did the nontransfected cells. These colonies contained only episomal virus DNA; therefore, integration of HPV sequences is important for immortalization of keratinocytes. This study suggests that the virus-encoded immortalization function contributes to the pathogenesis of cervical carcinoma.
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“using primary human keratinocytes, it was shown that high-risk HPV genomes cause lifespan extension, inhibit keratinocyte differentiation and lead to cellular immortalization [85,86,87]”
From The Not-So-Good, the Bad and the Ugly: HPV E5, E6 and E7 Oncoproteins in the Orchestration of Carcinogenesis (2021) · cites this paper as [87]
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Research works printed but not linked: 4
- Durst, M., L. Gissmann, H. Ikenberg, and H. zur Hausen. 1983. A papillomavirus DNA from a cervical carcinoma and its prevalence in cancer biopsy samples from different geographic regions. Proc. Natl. Acad. Sci. USA 80:3812-3815.
- LaPorta, R. F., and L. B. Taichman. 1982. Human papilloma viral DNA replicates as a stable episome in cultured epidermal keratinocytes. Proc. Natl. Acad. Sci. USA 79:3393-3397.
- Ostrow, R. S., M. Bender, M. Niimura, T. Seki, M. Kawashima, F. Pass, and A. J. Faras. 1982. Human papillomavirus DNA in cutaneous primary and metastasized squamous cell carcinomas from patients with epidermodysplasia verruciformis. Proc. Natl. Acad. Sci. USA 79:1634-1638.
- In B. M. Steinberg, J. L. Brandsma, and L. B. Taichman (ed.), Cancer cells, vol. 5. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
Cited by
Association of Human Papillomavirus Types 16 and 18 E6 Proteins with p53
Werness, B. A., Levine, A. J., & Howley, P. M. (1990) · Science · doi:10.1126/science.2157286 · Cited by 2,562
Basukala, O., & Banks, L. (2021) · Viruses · doi:10.3390/v13101892 · Cited by 137
Metadata
- Authors
- Woodworth, C. D., Doniger, J., & DiPaolo, J. A.
- Affiliations
- Division of Cancer Etiology, National Cancer Institute, Bethesda, Maryland 20892.
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 1989-01
- Volume
- 63
- Issue
- 1
- Pages
- 159-164
- ISSN
- 0022-538X
- 1098-5514
- Cited by
- 190
- References
- 0
- Language
- en
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- CoChat. (2026). Citation check: Immortalization of human foreskin keratinocytes by various human papillomavirus DNAs corresponds to their association with cervical carcinoma. https://app.cochat.ai/papers/10.1128/jvi.63.1.159-164.1989
- The paper
- Woodworth, C. D., Doniger, J., & DiPaolo, J. A. (1989). Immortalization of human foreskin keratinocytes by various human papillomavirus DNAs corresponds to their association with cervical carcinoma. Journal of Virology. https://doi.org/10.1128/jvi.63.1.159-164.1989
