The DOI matches its Crossref record; 32 of the 32 references it cites were found in a registry or an index.
Mutations that alter an Arg-Gly-Asp (RGD) sequence in the adenovirus type 2 penton base protein abolish its cell-rounding activity and delay virus reproduction in flat cells
Open accessAbstract
The adenovirus penton base protein has a cell rounding activity and may lyse endosomes during virus entry into the cytoplasm. We found that penton base that was expressed in Escherichia coli also caused cell rounding and that cells adhered to polystyrene wells that were coated with the protein. Mutant analysis showed that both properties required an Arg-Gly-Asp (RGD) sequence at residues 340 to 342 of penton base. In flat adherent cells, virus mutants with amino acid substitutions in the RGD sequence were delayed in virus reproduction and in the onset of viral DNA synthesis. In nonadherent or poorly spread cells, the kinetics of mutant virus reproduction were similar to those of wild-type adenovirus type 2. Expression of the mutant phenotype exclusively in the flat cells that we tested supports a model in which penton base interacts with an RGD-directed cell adhesion molecule during adenovirus uptake or uncoating.
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Dynein- and microtubule-mediated translocation of adenovirus serotype 5 occurs after endosomal lysis
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Metadata
- Authors
- Bai, M., Harfe, B., & Freimuth, P.
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 1993-09
- Volume
- 67
- Issue
- 9
- Pages
- 5198-5205
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- OpenAlex
- W1857650329
- Cited by
- 274
- References
- 32
- FWCI
- 6.47
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Mutations that alter an Arg-Gly-Asp (RGD) sequence in the adenovirus type 2 penton base protein abolish its cell-rounding activity and delay virus reproduction in flat cells. https://app.cochat.ai/papers/10.1128/jvi.67.9.5198-5205.1993
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- Bai, M., Harfe, B., & Freimuth, P. (1993). Mutations that alter an Arg-Gly-Asp (RGD) sequence in the adenovirus type 2 penton base protein abolish its cell-rounding activity and delay virus reproduction in flat cells. Journal of Virology. https://doi.org/10.1128/jvi.67.9.5198-5205.1993
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
