The DOI matches its Crossref record; 59 of the 59 references it cites were found in a registry or an index.
Identification of a membrane-binding domain within the amino-terminal region of human immunodeficiency virus type 1 Gag protein which interacts with acidic phospholipids
Open accessAbstract
Retroviral Gag proteins are targeted to the plasma membrane, where they play the central role in virion formation. Several studies have suggested that the membrane-binding signal is contained within the amino-terminal matrix sequence; however, the precise location has never been determined for the Gag protein of any retrovirus. In this report, we show that the first 31 residues of human immunodeficiency virus type 1 Gag protein can function independently as a membrane-targeting domain when fused to heterologous proteins. A bipartite membrane-targeting motif was identified, consisting of the myristylated N-terminal 14 amino acids and a highly basic region that binds acidic phospholipids. Replacement of the N-terminal membrane-targeting domain of pp60v-src with that of human immunodeficiency virus type 1 Gag elicits efficient membrane binding and a transforming phenotype. Removal of myristate or the basic region results in decreased membrane binding of Gag-Src chimeras in vitro and impaired virion formation by Pr55gag in vivo. We propose that the N-terminal Gag sequence functions as a targeting signal to direct interaction with acidic phospholipids on the cytoplasmic leaflet of the plasma membrane.
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Cited by
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Self-citationHIV-1 Gag proteins: diverse functions in the virus life cycle
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The myristoyl-electrostatic switch: a modulator of reversible protein-membrane interactions
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Phosphatidylinositol (4,5) bisphosphate regulates HIV-1 Gag targeting to the plasma membrane
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The structural biology of HIV assembly
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Metadata
- Authors
- Zhou, W., Parent, L. J., Wills, J. W., & Resh, M. D.
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 1994-04
- Volume
- 68
- Issue
- 4
- Pages
- 2556-2569
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- OpenAlex
- W1579191879
- Cited by
- 530
- References
- 59
- FWCI
- 8.49
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: Identification of a membrane-binding domain within the amino-terminal region of human immunodeficiency virus type 1 Gag protein which interacts with acidic phospholipids. https://app.cochat.ai/papers/10.1128/jvi.68.4.2556-2569.1994
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- Zhou, W., Parent, L. J., Wills, J. W., & Resh, M. D. (1994). Identification of a membrane-binding domain within the amino-terminal region of human immunodeficiency virus type 1 Gag protein which interacts with acidic phospholipids. Journal of Virology. https://doi.org/10.1128/jvi.68.4.2556-2569.1994
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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