The DOI matches its Crossref record; 39 of the 39 references it cites were found in a registry or an index.
Human immunodeficiency virus type 1 viral protein R (Vpr) arrests cells in the G2 phase of the cell cycle by inhibiting p34cdc2 activity
Open accessAbstract
The Vpr accessory gene product of human immunodeficiency virus types 1 and 2 and simian immunodeficiency virus is believed to play a role in permitting entry of the viral core into the nucleus of nondividing cells. A second role for Vpr was recently suggested by Rogel et al. (M. E. Rogel, L. I. Wu, and M. Emerman, J. Virol. 69:882-888, 1995), who showed that Vpr prevents the establishment in vitro of chronically infected HIV producer cell lines, apparently by causing infected cells to arrest in the G2/M phase of the cell cycle. In cycling cells, progression from G2 to M phase is driven by activation of the p34cdc2/cyclin B complex, an event caused, in part, by dephosphorylation of two regulatory amino acids of p34cdc2 (Thr-14 and Tyr-15). We show here that Vpr arrests the cell cycle in G2 by preventing the activation of the p34cdc2/cyclin B complex. Vpr expression in cells caused p34cdc2 to remain in the phosphorylated, inactive state, p34cdc2/cyclin B complexes immunoprecipitated from cells expressing Vpr were almost completely inactive in a histone H1 kinase assay. Coexpression of a constitutively active mutant p34cdc2 molecule with Vpr relieved the G2 arrest. These findings strongly suggest that Vpr arrests cells in G2 by preventing the activation of the p34cdc2/cyclin B complex that is required for entry into M phase. In vivo, Vpr might, by preventing p34cdc2 activation, delay or prevent apoptosis of infected cells. This would increase the amount of virus each infected cell produced.
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Cited by
Regulation of NF-κB by Cyclin-Dependent Kinases Associated with the p300 Coactivator
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Cyclin-dependent kinase-1: linking apoptosis to cell cycle and mitotic catastrophe
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HIV-1 Vpr interacts with the nuclear transport pathway to promote macrophage infection
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Metadata
- Authors
- He, J., Choe, S., Walker, R., Di Marzio, P., Morgan, D. O., & Landau, N. R.
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 1995-11
- Volume
- 69
- Issue
- 11
- Pages
- 6705-6711
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- OpenAlex
- W1925504181
- Cited by
- 904
- References
- 39
- FWCI
- 9.22
- Type
- journal article
- Language
- en
- Open access
- green
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- He, J., Choe, S., Walker, R., Di Marzio, P., Morgan, D. O., & Landau, N. R. (1995). Human immunodeficiency virus type 1 viral protein R (Vpr) arrests cells in the G2 phase of the cell cycle by inhibiting p34cdc2 activity. Journal of Virology. https://doi.org/10.1128/jvi.69.11.6705-6711.1995
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