The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
A "humanized" green fluorescent protein cDNA adapted for high-level expression in mammalian cells
Open accessAbstract
We constructed gfph, a synthetic version of the jellyfish Aequorea victoria green fluorescent protein (gfp) cDNA that is adapted for high-level expression in mammalian cells, especially those of human origin. A total of 92 base substitutions were made in 88 codons in order to change the codon usage within the gfp10 coding sequence so that it was more appropriate for expression in mammalian cells. We also describe a series of versatile recombinant adeno-associated virus and adenovirus vectors for delivery and expression of genes into mammalian cells and, using these vectors, demonstrate the efficient transduction and expression of the gfph gene in the human cell line 293 and also in vivo, within neurosensory cells of guinea pig eye. Cells infected with recombinant adeno-associated virus-GFPH can be readily sorted by fluorescence-activated cell sorting, suggesting that the newly designed gfph gene could be widely used as a reporter in many gene delivery technologies, including human gene therapy.
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Cites
Adeno-associated virus general transduction vectors: analysis of proviral structures
McLaughlin, S. K., Collis, P., Hermonat, P. L., & Muzyczka, N. (1988) · Journal of Virology · doi:10.1128/jvi.62.6.1963-1973.1988 · Cited by 348
Self-citationSequence requirements for binding of Rep68 to the adeno-associated virus terminal repeats
Ryan, J. H., Zolotukhin, S., & Muzyczka, N. (1996) · Journal of Virology · doi:10.1128/jvi.70.3.1542-1553.1996 · Cited by 119
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Cited by
Subventricular zone astrocytes are neural stem cells in the adult mammalian brain
Doetsch, F., Caillé, I., Lim, D. A., García-Verdugo, J. M., & Alvarez-Buylla, A. (1999) · Cell · doi:10.1016/s0092-8674(00)80783-7 · Cited by 3,898
Codon bias and heterologous protein expression
Gustafsson, C., Govindarajan, S., & Minshull, J. (2004) · Trends in Biotechnology · doi:10.1016/j.tibtech.2004.04.006 · Cited by 1,330
Zolotukhin, S., Byrne, B. J., Mason, E., Zolotukhin, I., Potter, M., Chesnut, K., Summerford, C., Samulski, R. J., & Muzyczka, N. (1999) · Gene Therapy · doi:10.1038/sj.gt.3300938 · Cited by 1,312
Self-citationBurger, C., Gorbatyuk, O. S., Velardo, M. J., Peden, C. S., Williams, P., Zolotukhin, S., Reier, P. J., Mandel, R. J., & Muzyczka, N. (2004) · Molecular Therapy · doi:10.1016/j.ymthe.2004.05.024 · Cited by 741
Self-citationGrimm, D., Lee, J. S., Wang, L., Desai, T., Akache, B., Storm, T. A., & Kay, M. A. (2008) · Journal of Virology · doi:10.1128/jvi.00254-08 · Cited by 696
Metadata
- Authors
- Zolotukhin, S., Potter, M., Hauswirth, W. W., Guy, J., & Muzyczka, N.
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 1996-07
- Volume
- 70
- Issue
- 7
- Pages
- 4646-4654
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- OpenAlex
- W1490724066
- Cited by
- 609
- References
- 2
- FWCI
- 30.28
- Type
- journal article
- Language
- en
- Open access
- green
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- Zolotukhin, S., Potter, M., Hauswirth, W. W., Guy, J., & Muzyczka, N. (1996). A "humanized" green fluorescent protein cDNA adapted for high-level expression in mammalian cells. Journal of Virology. https://doi.org/10.1128/jvi.70.7.4646-4654.1996
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