Adeno-associated virus general transduction vectors: analysis of proviral structures
Open accessAbstract
We used two kinds of adeno-associated virus (AAV) vectors to transduce the neomycin resistance gene into human cells. The first of these (dl52-91) retains the AAV rep genes; the second (dl3-94) retains only the AAV terminal repeats and the AAV polyadenylation signal (428 base pairs). Both vectors could be packaged into AAV virions and produced proviral structures that were essentially the same. Thus, the AAV sequences that are required in cis for packaging (pac), integration (int), rescue (res), and replication (ori) of viral DNA are located within a 284-base-pair sequence that includes the terminal repeat. Most of the G418r cell lines (73%) contained proviruses which could be rescued (Res+) when the cells were superinfected with the appropriate helper viruses. Some produced high yields of viral DNA; other rescued at a 50-fold lower level. Most of the lines that were Res+ (79%) contained a tandem repeat of the AAV genome (2 to 20 copies) which was integrated randomly with respect to cellular DNA. Junctions between two consecutive AAV copies in a tandem array contained either one or two copies of the AAV terminal palindrome. Junctions between AAV and cellular sequences occurred predominantly at or within the AAV terminal repeat, but in some cases at internal AAV sequences. Two lines were seen that contained free episomal copies of AAV DNA. Res+ clones contained deleted proviruses or tandem repeats of a deleted genome. Occasionally, flanking cellular DNA was also amplified. The…
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The viral capsid is assembled from the overlapping VP3 region.
“The viral capsid is assembled from the overlapping VP3 region [8].”
From Understanding Capsid Assembly and Genome Packaging for Adeno-Associated Viruses (2017) · cites this paper as [8]
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- Authors
- McLaughlin, S. K., Collis, P., Hermonat, P. L., & Muzyczka, N.
- Affiliations
- University of Florida Health (US)
- ORCID
- Collis, P
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 1988-06
- Volume
- 62
- Issue
- 6
- Pages
- 1963-1973
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- PMC
- PMC253280
- OpenAlex
- W1699538093
- Cited by
- 348
- References
- 1
- Citations by year
- 2025: 2 · 2024: 2 · 2023: 7 · 2022: 1 · 2021: 7 · 2020: 3 · 2019: 7 · 2018: 4 · 2017: 4 · 2016: 3 · 2015: 10 · 2014: 4 · 2013: 5 · 2012: 1
- FWCI
- 4.66
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Virology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of General Medical Sciences (R01GM3572302)
- National Institute of Allergy and Infectious Diseases (5-T32 AI 07110, T32AI007110)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Keywords
- adeno-associated virus, viral DNA integration, superinfection, viral DNA replication, tandem repeats, neomycin resistance gene, helper virus, G418 resistance
- MeSH terms
- Dependovirus, Cell Line, Chromosome Mapping, Cloning, Molecular, DNA Replication, Genes, Viral, Genetic Vectors, Helper Viruses, Humans, Plasmids, Recombination, Genetic, Regulatory Sequences, Nucleic Acid, Repetitive Sequences, Nucleic Acid, Selection, Genetic, Transduction, Genetic, Virus Replication
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