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Mass Determination of Rous Sarcoma Virus Virions by Scanning Transmission Electron Microscopy
Abstract
The internal structural protein of retroviruses, Gag, comprises most of the mass of the virion, and Gag itself can give rise to virus-like particles when expressed in appropriate cells. Previously the stoichiometry of Gag in virions was inferred from indirect measurements carried out 2 decades ago. We now have directly determined the masses of individual particles of the prototypic avian retrovirus, Rous sarcoma virus (RSV), by using scanning transmission electron microscopy. In this technique, the number of scattered electrons in the dark-field image integrated over an individual freeze-dried virus particle on a grid is directly proportional to its mass. The RSV virions had a mean mass of 2.5 × 10 8 Da, corresponding to about 1,500 Gag molecules per virion. The population of virions was not homogeneous, with about one-third to two-thirds of the virions deviating from the mean by more than 10% of the mass in two respective preparations. The mean masses for virions carrying genomes of 7.4 or 9.3 kb were indistinguishable, suggesting that mass variability is not due to differences in RNA incorporation.
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The packaging capacity of the lentiviral vector is around 8 kb.
“The packaging capacity of the LV is around 8 kb [43].”
From Viral Delivery Systems for CRISPR (2019) · cites this paper as [43]
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Cites
Specific incorporation of cyclophilin A into HIV-1 virions
Franke, E. K., Yuan, H. E. H., & Luban, J. (1994) · Nature · doi:10.1038/372359a0 · Cited by 745
Flagged first, then by citations
Research works printed but not linked: 39
- 10.1073/pnas.90.11.5181 · DOI
- 10.1126/science.1470916 · DOI
- 10.1016/S0065-230X(08)60982-3 · DOI
- Bonar R. A. Beard J. W. Virus of avian myeloblastosis. XII. Chemical constitution.J. Natl. Cancer Inst. 23 1959 183 195
- 10.1128/jvi.69.10.6487-6497.1995 · DOI
- 10.1128/jvi.71.6.4425-4435.1997 · DOI
- 10.1016/0042-6822(68)90118-9 · DOI
- 10.1016/0042-6822(70)90428-9 · DOI
- 10.1016/0042-6822(69)90313-4 · DOI
- 10.1128/jvi.12.2.334-342.1973 · DOI
- 10.1128/mcb.13.4.2604-2613.1993 · DOI
- 10.1128/jvi.8.5.778-785.1971 · DOI
- 10.1016/S0960-9822(06)00331-9 · DOI
- 10.1128/JVI.72.6.4667-4677.1998 · DOI
- 10.1128/JVI.73.3.2309-2320.1999 · DOI
- 10.1126/science.2416054 · DOI
- 10.1128/JVI.72.1.564-577.1998 · DOI
- 10.1089/aid.1995.11.1003 · DOI
- 10.1128/jvi.70.11.7734-7743.1996 · DOI
- 10.1128/jvi.45.2.648-658.1983 · DOI
- 10.1128/jvi.58.1.50-58.1986 · DOI
- 10.1128/jvi.69.10.6430-6438.1995 · DOI
- 10.1128/jvi.70.5.3313-3318.1996 · DOI
- 10.1128/jvi.65.7.3728-3737.1991 · DOI
- 10.1128/jvi.66.6.3391-3397.1992 · DOI
- 10.1016/0042-6822(90)90035-P · DOI
- 10.1016/0042-6822(71)90010-9 · DOI
- 10.1006/viro.1996.0343 · DOI
- 10.1016/0042-6822(71)90049-3 · DOI
- 10.1073/pnas.58.3.825 · DOI
- 10.1128/jvi.12.6.1226-1237.1973 · DOI · Paper page
- 10.1128/jvi.34.2.431-437.1980 · DOI
- 10.1128/jvi.64.10.5076-5092.1990 · DOI
- 10.1128/jvi.13.2.513-528.1974 · DOI
- 10.1038/372363a0 · DOI
- 10.1128/jvi.54.2.598-607.1985 · DOI
- 10.1016/0022-2836(75)90174-6 · DOI
- 10.1016/S0091-679X(08)60878-X · DOI
- 10.1073/pnas.95.13.7299 · DOI · Paper page
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Self-citationStructure of the N-terminal 283-residue fragment of the immature HIV-1 Gag polyprotein
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Beyond reverse transcription: molecular mechanisms and emerging paradigms in retroviral replication
Jehad, M. A., Ali, L. M., Pillai, V. N., Prabhu, S. G., Mustafa, F., & Rizvi, T. A. (2026) · FEMS Microbiology Reviews · doi:10.1093/femsre/fuaf066 · Cited by 5
“…the fundamental building block for retroviral particle assembly, often referred to as an “assembly machine,” where each assembling virus particle may contain as many as 2000–5000 Gag molecules (Wills et al. 1991 , Vogt and Simon 1999 , Briggs et al. 2004 , Carlson et al. 2008 , Chen et al. 2009 ).”
Metadata
- Authors
- Vogt, V. M., & Simon, M. N.
- Affiliations
- <!--label omitted: 1-->Section of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853,1and
- <!--label omitted: 2-->Department of Biology, Brookhaven National Laboratory, Upton, New York 119732
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 1999-08
- Volume
- 73
- Issue
- 8
- Pages
- 7050-7055
- ISSN
- 0022-538X
- 1098-5514
- Cited by
- 118
- References
- 1
- Language
- en
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