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Infection of Dendritic Cells (DCs), Not DC-SIGN-Mediated Internalization of Human Immunodeficiency Virus, Is Required for Long-Term Transfer of Virus to T Cells
Open accessAbstract
The C-type lectin DC-SIGN expressed on immature dendritic cells (DCs) captures human immunodeficiency virus (HIV) particles and enhances the infection of CD4 + T cells. This process, known as trans -enhancement of T-cell infection, has been related to HIV endocytosis. It has been proposed that DC-SIGN targets HIV to a nondegradative compartment within DCs and DC-SIGN-expressing cells, allowing incoming virus to persist for several days before infecting target cells. In this study, we provide several lines of evidence suggesting that intracellular storage of intact virions does not contribute to HIV transmission. We show that endocytosis-defective DC-SIGN molecules enhance T-cell infection as efficiently as their wild-type counterparts, indicating that DC-SIGN-mediated HIV internalization is dispensable for trans -enhancement. Furthermore, using immature DCs that are genetically resistant to infection, we demonstrate that several days after viral uptake, HIV transfer from DCs to T cells requires viral fusion and occurs exclusively through DC infection and transmission of newly synthesized viral particles. Importantly, our results suggest that DC-SIGN participates in this process by cooperating with the HIV entry receptors to facilitate cis -infection of immature DCs and subsequent viral transfer to T cells. We suggest that such a mechanism, rather than intracellular storage of incoming virus, accounts for the long-term transfer of HIV to CD4 + T cells and may contribute to the spread of infection by DCs.
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“Later, immature DCs become productively infected, and transmit viral particles produced de novo to T cells in the cis-infection process [66,67,69,70].”
From HIV-1 and HTLV-1 Transmission Modes: Mechanisms and Importance for Virus Spread (2022) · cites this paper as [69]
Citations
Cites
Recruitment of HIV and Its Receptors to Dendritic Cell-T Cell Junctions
McDonald, D., Wu, L., Bohks, S. M., KewalRamani, V. N., Unutmaz, D., & Hope, T. J. (2003) · Science · doi:10.1126/science.1084238 · Cited by 707
DC-SIGN-mediated internalization of HIV is required for trans-enhancement of T cell infection
Kwon, D. S., Gregorio, G., Bitton, N., Hendrickson, W. A., & Littman, D. R. (2002) · Immunity · doi:10.1016/s1074-7613(02)00259-5 · Cited by 523 · cited 7×
“ments within immature DCs and DC-SIGN-expressing cells (13).”
BackgroundMethodResultInfluentialZaitseva, M., Blauvelt, A., Lee, S., Lapham, C. K., Kiaus-Kovrun, V., Mostowski, H., Manischewitz, J., & Golding, H. (1997) · Nature Medicine · doi:10.1038/nm1297-1369 · Cited by 389
Research works printed but not linked: 35
- 10.1128/JVI.77.4.2550-2558.2003 · DOI
- 10.1182/blood.V93.11.3866 · DOI
- 10.1073/pnas.89.17.8356 · DOI
- 10.1038/40894 · DOI
- 10.4049/jimmunol.168.5.2118 · DOI
- 10.1111/j.1600-0854.2005.00293.x · DOI
- 10.1016/S0092-8674(00)80694-7 · DOI · Paper page
- 10.1128/JVI.72.4.2733-2737.1998 · DOI
- 10.1016/S1074-7613(02)00447-8 · DOI
- 10.1128/JVI.74.13.6087-6095.2000 · DOI
- 10.1073/pnas.1432450100 · DOI
- 10.1016/j.immuni.2005.04.004 · DOI
- 10.1126/science.1099288 · DOI
- 10.1128/JVI.75.24.12028-12038.2001 · DOI
- 10.1084/jem.20042413 · DOI
- 10.1074/jbc.M504337200 · DOI
- 10.1074/jbc.M301284200 · DOI
- 10.1016/j.virol.2005.05.023 · DOI
- 10.1016/S0092-8674(01)00449-4 · DOI
- 10.1182/blood-2003-07-2532 · DOI
- 10.1128/JVI.79.9.5386-5399.2005 · DOI · Paper page
- 10.1006/viro.2001.0984 · DOI
- 10.1128/JVI.78.2.798-810.2004 · DOI
- 10.1128/JVI.75.10.4664-4672.2001 · DOI
- 10.1016/0092-8674(94)90418-9 · DOI
- 10.1038/382722a0 · DOI
- 10.1016/S1074-7613(02)00260-1 · DOI
- 10.1084/jem.183.1.215 · DOI
- 10.1074/jbc.M003868200 · DOI
- 10.1093/intimm/dxg030 · DOI
- 10.1038/ni841 · DOI
- 10.1182/blood-2003-09-3129 · DOI · Paper page
- 10.1038/nri1182 · DOI
- 10.1038/nature04055 · DOI
- 10.1016/S0092-8674(00)80828-4 · DOI
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Saïdi, H., Jenabian, M.-A., Gombert, B., Charpentier, C., Mannarini, A., & Bélec, L. (2008) · AIDS Research and Therapy · doi:10.1186/1742-6405-5-10 · Cited by 11
Shinya, E., Shimizu, M., Owaki, A., Paoletti, S., Mori, L., Libero, G. D., & Takahashi, H. (2016) · Virology · doi:10.1016/j.virol.2015.10.023 · Cited by 11
Lehman, T. L., O'Halloran, K. P., Hoover, E., & Avery, P. (2009) · Veterinary Immunology and Immunopathology · doi:10.1016/j.vetimm.2009.10.012 · Cited by 10
“Whether productive infection is a required component of long-term in vivo persistence is unresolved (Burleigh et al., 2006; Granelli-Piperno et al., 2006).”
Biggins, J. E., Biesinger, T., Kimata, M. T. Y., Arora, R., & Kimata, J. T. (2007) · Virology · doi:10.1016/j.virol.2007.03.017 · Cited by 9
“Alternately, infection of DCs may lead to de novo replication of the virus for transmission to CD4+ T cells (Burleigh et al., 2006;Nobile et al., 2005).”
BackgroundVenkatachari, N. J., Alber, S. M., Watkins, S. C., & Ayyavoo, V. (2009) · PLoS ONE · doi:10.1371/journal.pone.0007470 · Cited by 8
“DC interact with pathogen infected/exposed cells in various tissue compartments as part of the immune surveillance function in vivo [16,17].”
BackgroundThe evolution of HIV-1 interactions with coreceptors and mannose C-type lectin receptors.
Borggren, M., & Jansson, M. (2015) · Progress in Molecular Biology and Translational Science · doi:10.1016/bs.pmbts.2014.10.004 · Cited by 9
HIV Impairment of Immune Responses in Dendritic Cells
Ahmed, Z., Czubala, M. A., Blanchet, F. P., & Piguet, V. (2012) · Advances in experimental medicine and biology · doi:10.1007/978-1-4614-4433-6_8 · Cited by 10
Identification of the DC-SIGN-Interactive Domains on the Envelope Glycoprotein of HIV-1 CRF07_BC
Liao, C.-F., Wang, S.-F., Lin, Y.-T., Ho, D., & Chen, Y. (2011) · AIDS Research and Human Retroviruses · doi:10.1089/aid.2010.0215 · Cited by 7
“11,12 Identifying the DC-SIGN-interactive domains on the envelope glycoprotein of HIV-1 may shed light on the viral attachment process and provide information for the development of therapeutic agents and preventive vaccines for HIV-1 infection.”
Ilinskaya, A. N., Heidecker, G., & Jones, K. S. (2010) · Future Medicinal Chemistry · doi:10.4155/fmc.10.255 · Cited by 6
Current concepts of HIV transmission
Morrow, G., Vachot, L., Vagenas, P., & Robbiani, M. (2008) · Current Infectious Disease Reports · doi:10.1007/s11908-008-0024-9 · Cited by 15
“The rapid transfer of captured virus by immature and mature DCs versus the delayed second phase transfer of newly produced virus by immature DCs [19, 29 ] is probably responsible for the massive amplifi cation of virus observed in T cells in vivo (in local tissues versus draining lymphoid tissues).”
BackgroundHIV-1 Border Patrols: Langerhans Cells Control Antiviral Responses and Viral Transmission
Ribeiro, C. M. S., Sarrami‐Forooshani, R., & Geijtenbeek, T. B. H. (2015) · Future Virology · doi:10.2217/fvl.15.79 · Cited by 7
Comparative Analysis of Tat-Dependent and Tat-Deficient Natural Lentiviruses
Bose, D., Gagnon, J., & Chebloune, Y. (2015) · Veterinary Sciences · doi:10.3390/vetsci2040293 · Cited by 7
“to capture the virus through lectin-type receptors such as DC-specific ICAM-3-grabbing non-integrin (DC-SIGN) or sialic acid-binding Ig-like lectin 1 (SIGLEC-1), and act primarily as intermediates for virus transmission to T cells since HIV-1 replication in these lineages is limited [107–109].”
BackgroundSalim, A., & Ratner, L. (2008) · Journal of Virology · doi:10.1128/jvi.00430-07 · Cited by 5
“However, there are high levels of E-cadherin in macrophages and dendritic cells, in which these effects of Vpu are likely to be important for pathogenesis (6, 23, 25, 37, 62).”
BackgroundDendritic cell function in HIV infection
Miller, E. A., Bhardwaj, N., & O'Brien, M. (2009) · HIV Therapy · doi:10.2217/hiv.09.34 · Cited by 2
Attachment of human immunodeficiency virus to cells and its inhibition
Pöhlmann, S., & Tremblay, M. J. (2007) · Birkhäuser Basel eBooks · doi:10.1007/978-3-7643-7783-0_3 · Cited by 4
“Moreover, it has been demonstrated that transformed cells frequently used to assess DC-SIGN function were not THP-1 monocytes, as reported [10], but most likely Raji B-cells [73] , and that these cells as well as monocyte-derived DCs were permissive to infection by HIV-1 [74] [75] [76] .”
BackgroundJanevska, M., Witkowski, W., Vermeíre, J., Borowicz, M., Naessens, E., Vanderstraeten, H., Nauwynck, H., Favoreel, H. W., & Verhasselt, B. (2025) · Journal of Virology · doi:10.1128/jvi.02066-24
“Dendritic cells have a denser actin cytoskeleton and several surface molecules with higher affinity for the HIV-1 viral envelope than the CD4 receptor that capture HIV in surface-bound compartments, making endocytosis an important restriction mechanism in DCs (16, 57).”
Mucosal Dendritic Cells in HIV-1 Susceptibility: A Critical Role for C-type Lectin Receptors
Hertoghs, N., van Pul, L., & Geijtenbeek, T. B. H. (2017) · Future Virology · doi:10.2217/fvl-2017-0020 · Cited by 1
Shiohara, T., Mizukawa, Y., & Kano, Y. (2010) · Dermatology · doi:10.1159/000277447 · Cited by 1
Lectin-like interactions in virus–cell recognition
Steffen, I., Tsegaye, T. S., & Pöhlmann, S. (2010) · Elsevier eBooks · doi:10.1016/b978-0-12-374546-0.00028-6 · Cited by 1
“However, subsequent studies could not confirm a role for intracellular acidic pH or DC-SIGN internalization in trans-infection (Nobile et al., 2005; Burleigh et al., 2006) .”
BackgroundInnate Recognition of HIV-1 Glycans: Implications for Infection, Transmission, and Immunity
van der Aar, A. M. G., Gringhuis, S. I., & Geijtenbeek, T. B. H. (2013) · Springer eBooks · doi:10.1007/978-1-4614-8872-9_2 · Cited by 1
“One study showed that the LL motif in the cytoplasmic tail of DC-SIGN does not contribute to HIV-1 transmission by DC-SIGN-expressing cell lines (Burleigh et al. 2006 ), indicating that uptake is not required for HIV-1 trans -infection.”
Background73 – Human Immunodeficiency Virus and Allergic Disease
Nicholas, S., Paul, M., & Shearer, W. (2014) · doi:10.1016/b978-0-323-08593-9.00074-7 · Cited by 1
Abdollahi, M., Rasizadeh, R., Eslami, N., Asbagh, A. G., Noroozi, V., Shokri, S., & Baghi, H. B. (2025) · Apmis · doi:10.1111/apm.70084
Wang, W., Truong, K., Ye, C., Sharma, S., He, H., Liu, L., Wen, M., Misra, A., Zhou, P., & Kimata, J. T. (2023) · Frontiers in Immunology · doi:10.3389/fimmu.2023.1167965 · Cited by 1
Resolving the Sites of HIV Entry: Dynamin Networks Hold the Key
Aggarwal, A., & Turville, S. G. (2017) · Journal of Cell Signaling · doi:10.4172/2576-1471.1000173
Baculovirus Infection of Human Monocyte-Derived Dendritic Cells Restricts HIV-1 Replication
Chang, M. O., Suzuki, T., Kitajima, M., & Takaku, H. (2015) · AIDS Research and Human Retroviruses · doi:10.1089/aid.2015.0060
“7,8 Although DCs are susceptible to HIV-1, viral replication is generally less productive in DCs than in CD4 + T cells, presumably because of the presence in DCs of cellular restriction factors, such as apo-lipoprotein B mRNA-editing catalytic polypeptide (APO-BEC3), cyclophilin A, and SAMHD1,…”
Sutkevičiu̅tė, I. (2012) · theses.fr (ABES) · doi:10.70675/0285a9b5z7649z4316za5eez803884af1c47
Dendritic Cell-Mediated Viral Transmission: A Potential Drug Target?
de Witte, L. D., van Kooyk, Y., & Geijtenbeek, T. B. H. (2006) · Future Virology · doi:10.2217/17460794.1.5.615
“Several days later, de novo produced virus particles in DCs can be transferred to target cells, presumably also via the infectious synapse [10,14].”
BackgroundResultInfluentialCharacterization of Innate Cellular Modulators of Retroviral Infection
Eriksson, E. (2015) · doi:10.11588/heidok.00018685
“The latter one is supposed to involve the de novo pathway of viral transmission, while the early phase trans-infection is characterized by transfer of primarily surface-bound HIV particles to CD4+ T cells (21, 26).”
Background
Metadata
- Authors
- Burleigh, L., Lozach, P.-Y., Schiffer, C., Staropoli, I., Pezo, V., Porrot, F., Canque, B., Virelizier, J.-L., Arenzana-Seisdedos, F., & Amara, A.
- Affiliations
- Unité d'Immunologie Virale
- Centre National de la Recherche Scientifique (FR)
- CNRS UMR 7151 IUH, Centre Hayem, Hôpital Saint Louis, 1 avenue Claude Vellefaux, 75475 Paris Cedex 10, France
- Institut Curie INSERM U520, 12 rue Lhomond, 75005 Paris, France
- Groupe Virus et Immunité, Institut Pasteur, 28 rue du Dr. Roux, 75724 Paris, France
- Corresponding author
- Amara, Ali
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 2006-03-15
- Volume
- 80
- Issue
- 6
- Pages
- 2949-2957
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- PMC
- PMC1395470
- OpenAlex
- W1983080372
- Cited by
- 151
- References
- 0
- Citations by year
- 2025: 2 · 2023: 2 · 2022: 2 · 2021: 2 · 2020: 3 · 2019: 2 · 2018: 5 · 2017: 6 · 2016: 6 · 2015: 4 · 2014: 7 · 2013: 9 · 2012: 12
- FWCI
- 8.10
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Journal of Virology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
- Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
- Health Sciences › Medicine › Infectious Diseases › HIV/AIDS Research and Interventions
- Keywords
- HIV, dendritic cells, DC-SIGN, endocytosis, viral internalization, viral fusion, CD4+ T cells, HIV transmission, C-type lectin
- MeSH terms
- DC-Specific ICAM-3 Grabbing Nonintegrin, Amino Acid Sequence, Animals, Cell Line, Dendritic Cells, Humans, Molecular Sequence Data, Receptors, Cell Surface, CD4-Positive T-Lymphocytes, HIV-1, HIV Infections, Cell Adhesion Molecules, Lectins, C-Type
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