The DOI matches its Crossref record; 60 of the 60 references it cites were found in a registry or an index.
Analysis of equid herpesvirus 1 strain variation reveals a point mutation of the DNA polymerase strongly associated with neuropathogenic versus nonneuropathogenic disease outbreaks.
Open accessAbstract
Equid herpesvirus 1 (EHV-1) can cause a wide spectrum of diseases ranging from inapparent respiratory infection to the induction of abortion and, in extreme cases, neurological disease resulting in paralysis and ultimately death. It has been suggested that distinct strains of EHV-1 that differ in pathogenic capacity circulate in the field. In order to investigate this hypothesis, it was necessary to identify genetic markers that allow subgroups of related strains to be identified. We have determined all of the genetic differences between a neuropathogenic strain (Ab4) and a nonneuropathogenic strain (V592) of EHV-1 and developed PCR/sequencing procedures enabling differentiation of EHV-1 strains circulating in the field. The results indicate the occurrence of several major genetic subgroups of EHV-1 among isolates recovered from outbreaks over the course of 30 years, consistent with the proposal that distinct strains of EHV-1 circulate in the field. Moreover, there is evidence that certain strain groups are geographically restricted, being recovered predominantly from outbreaks occurring in either North America or Europe. Significantly, variation of a single amino acid of the DNA polymerase is strongly associated with neurological versus nonneurological disease outbreaks. Strikingly, this variant amino acid occurs at a highly conserved position for herpesvirus DNA polymerases, suggesting an important functional role.
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“It was demonstrated that more than 86% of neuropathogenic outbreaks are caused by strains encoding D752 and more than 95% of non-neuropathogenic outbreaks were caused by virus strains encoding DNA pol N752.”
From The Pathogenesis and Immune Evasive Mechanisms of Equine Herpesvirus Type 1 (2021) · cites this paper as Nugent et al., 2006
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“The neurovirulent EHV-1 strain presents a single nucleotide polymorphism (SNP) in the catalytic subunit of the viral DNA polymerase (ORF30), causing a substitution of asparagine (N) by aspartic acid (D) at amino acid position 752 (Nugent et al., 2006).”
From The Pathogenesis and Immune Evasive Mechanisms of Equine Herpesvirus Type 1 (2021) · cites this paper as Nugent et al., 2006
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A point mutation in a herpesvirus polymerase determines neuropathogenicity.
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Metadata
- Authors
- Nugent, J., Birch-Machin, I., Smith, K. C., Mumford, J. A., Swann, Z., Newton, J. R., Bowden, R. J., Allen, G. P., & Davis-Poynter, N.
- Affiliations
- Animal Health Trust (GB)
- Animal Health Trust, Kentford, Newmarket, United Kingdom
- University of Oxford (GB)
- University of Kentucky (US)
- Corresponding author
- Davis-Poynter, N.
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 2006-04-15
- Volume
- 80
- Issue
- 8
- Pages
- 4047-4060
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- PMC
- PMC1440451
- OpenAlex
- W2167022679
- Cited by
- 295
- References
- 60
- Citations by year
- 2026: 2 · 2025: 13 · 2024: 14 · 2023: 9 · 2022: 15 · 2021: 20 · 2020: 14 · 2019: 23 · 2018: 11 · 2017: 20 · 2016: 11 · 2015: 15 · 2014: 14 · 2013: 15 · 2012: 15
- FWCI
- 10.74
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Journal of Virology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Herpesvirus Infections and Treatments
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Virus Infections Studies
- Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
- Keywords
- equine herpesvirus 1, neurovirulence, genetic subgroups, strain differentiation, neurological disorders, equine herpesvirus
- MeSH terms
- Amino Acid Sequence, Animals, Base Sequence, Disease Outbreaks, DNA-Directed DNA Polymerase, Herpesvirus 1, Equid, Horse Diseases, Horses, Molecular Sequence Data, Open Reading Frames, Point Mutation, Polymorphism, Single Nucleotide
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