Molecular Basis for the Evolution of Species-Specific Hemoglobin Capture by Staphylococcus aureus
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Abstract
During infection, bacteria must steal metals, including iron, from the host tissue. Therefore, pathogenic bacteria have evolved metal acquisition systems to overcome the elaborate processes mammals use to withhold metal from pathogens. Staphylococcus aureus uses IsdB, a hemoglobin receptor, to thieve iron-containing heme from hemoglobin within human blood. We find evidence that primate hemoglobin has undergone rapid evolution at protein surfaces contacted by IsdB. Additionally, variation in the hemoglobin sequences among primates, or variation in IsdB of related staphylococci, reduces bacterial hemoglobin capture. Together, these data suggest that S. aureus has evolved to recognize human hemoglobin in the face of rapid evolution at the IsdB binding interface, consistent with repeated evolutionary conflicts in the battle for iron during host-pathogen interactions.
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- Authors
- Choby, J. E., Buechi, H. B., Farrand, A. J., Skaar, E. P., & Barber, M. F.
- Affiliations
- Vanderbilt University (US)
- University of Oregon (US)
- Vanderbilt University Medical Center (US)
- Corresponding author
- Skaar, Eric P.
- Barber, Matthew F.
- Venue
- mBio
- Publisher
- American Society for Microbiology
- Published
- 2018-12-21
- Volume
- 9
- Issue
- 6
- ISSN
- 2150-7511 (ISSN-L)
- 2161-2129
- PMC
- PMC6247092
- OpenAlex
- W2901073394
- Cited by
- 15
- References
- 60
- Citations by year
- 2024: 1 · 2023: 2 · 2022: 3 · 2021: 2 · 2020: 6 · 2019: 1
- FWCI
- 1.70
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- mBio (publishedVersion, cc-by)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- HHS | NIH | National Institute of Allergy and Infectious Diseases (F31AI126662, R01AI069233, R01AI073843)
- HHS | NIH | National Institute of General Medical Sciences (T32GM065086, R00GM115822)
- HHS | NIH | National Institute of Diabetes and Digestive and Kidney Diseases (5U54DK110858)
- Donald E. and Delia B. Baxter Foundation
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antimicrobial Resistance in Staphylococcus
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Hemoglobin structure and function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- Staphylococcus aureus, ISDB, hemoglobin receptor, host-pathogen arms race, iron acquisition, host defense against bacteria
- MeSH terms
- Animals, Hemoglobins, Iron, Mutation, Primates, Protein Binding, Species Specificity, Staphylococcus aureus, Evolution, Molecular, Cation Transport Proteins, Host-Pathogen Interactions
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