The DOI matches its Crossref record; 62 of the 62 references it cites were found in a registry or an index.
P-Body Formation Is a Consequence, Not the Cause, of RNA-Mediated Gene Silencing
Open accessAbstract
P bodies are cytoplasmic domains that contain proteins involved in diverse posttranscriptional processes, such as mRNA degradation, nonsense-mediated mRNA decay (NMD), translational repression, and RNA-mediated gene silencing. The localization of these proteins and their targets in P bodies raises the question of whether their spatial concentration in discrete cytoplasmic domains is required for posttranscriptional gene regulation. We show that processes such as mRNA decay, NMD, and RNA-mediated gene silencing are functional in cells lacking detectable microscopic P bodies. Although P bodies are not required for silencing, blocking small interfering RNA or microRNA silencing pathways at any step prevents P-body formation, indicating that P bodies arise as a consequence of silencing. Consistently, we show that releasing mRNAs from polysomes is insufficient to trigger P-body assembly: polysome-free mRNAs must enter silencing and/or decapping pathways to nucleate P bodies. Thus, even though P-body components play crucial roles in mRNA silencing and decay, aggregation into P bodies is not required for function but is instead a consequence of their activity.
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“Furthermore, P-bodies form in an RNA-dependent manner as a consequence of RNAi and RNA degradation (80).”
From Overview of MicroRNA Biogenesis, Mechanisms of Actions, and Circulation (2018) · cites this paper as [80]
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MicroRNA-dependent localization of targeted mRNAs to mammalian P-bodies
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FMRP safeguards long mRNA expression by opposing repressive RNP statesPreprint
Ma, K., Hossain, A. K. M. M., Judson, K. L., Cheng, T., & Greenblatt, E. J. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.07.23.739932
N4BP1 uses tandem KH domains to associate with EDC4 and mRNA decapping factors in P-bodies
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The RNA-binding proteins Ddx6 and Ddx61 support development in mRNA-decay deficient pnrc2 mutants
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Metadata
- Authors
- Eulalio, A., Behm-Ansmant, I., Schweizer, D., & Izaurralde, E.
- Affiliations
- MPI for Developmental Biology, Spemannstrasse 35, D-72076 Tübingen, Germany
- Max Planck Institute for Developmental Biology (DE)
- Corresponding author
- Izaurralde, Elisa
- ORCID
- Izaurralde, Elisa
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 2007-06-01
- Volume
- 27
- Issue
- 11
- Pages
- 3970-3981
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- PMC
- PMC1900022
- OpenAlex
- W2101555571
- Cited by
- 710
- References
- 62
- Citations by year
- 2026: 18 · 2025: 20 · 2024: 25 · 2023: 26 · 2022: 34 · 2021: 33 · 2020: 30 · 2019: 26 · 2018: 31 · 2017: 29 · 2016: 30 · 2015: 32 · 2014: 40 · 2013: 45 · 2012: 58
- FWCI
- 22.81
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Molecular and Cellular Biology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion, other-oa)
- Max Planck Institute for Plasma Physics (submittedVersion)
- Funders
- European Molecular Biology Organization
- Max-Planck-Gesellschaft
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Keywords
- P-bodies, RNA-mediated gene silencing, siRNA, microRNA, nonsense-mediated mRNA decay, mRNA degradation, post-transcriptional regulation, polysome association, mRNA decapping
- MeSH terms
- Animals, Drosophila melanogaster, Humans, Polyribosomes, Ribonucleoproteins, RNA, Messenger, Gene Silencing, RNA Stability, Cytoplasmic Structures, Drosophila Proteins, RNA, Small Interfering, MicroRNAs
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