The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
Certain and Progressive Methylation of Histone H4 at Lysine 20 during the Cell Cycle
Open accessAbstract
Methylation of histone H4 at lysine 20 (K20) has been implicated in transcriptional activation, gene silencing, heterochromatin formation, mitosis, and DNA repair. However, little is known about how this modification is regulated or how it contributes to these diverse processes. Metabolic labeling and top-down mass spectrometry reveal that newly synthesized H4 is progressively methylated at K20 during the G(2), M, and G(1) phases of the cell cycle in a process that is largely inescapable and irreversible. Approximately 98% of new H4 becomes dimethylated within two to three cell cycles, and K20 methylation turnover in vivo is undetectable. New H4 is methylated regardless of prior acetylation, and acetylation occurs predominantly on K20-dimethylated H4, refuting the hypothesis that K20 methylation antagonizes H4 acetylation and represses transcription epigenetically. Despite suggestions that it is required for normal mitosis and cell cycle progression, K20 methylation proceeds normally during colchicine treatment. Moreover, delays in PR-Set7 synthesis and K20 methylation which accompany altered cell cycle progression during sodium butyrate treatment appear to be secondary to histone hyperacetylation or other effects of butyrate since depletion of PR-Set7 did not affect cell cycle progression. Together, our data provide an unbiased perspective of the regulation and function of K20 methylation.
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Cites
Chromatin modifications and their function
Kouzarides, T. (2007) · Cell · doi:10.1016/j.cell.2007.02.005 · Cited by 10,883
High-Resolution Profiling of Histone Methylations in the Human Genome
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“However, results from a recent genome-wide mapping study suggest that both 1m and 2mK4-H3 are preferentially enriched in chromatin flanking the promoters of active genes ( 2 ).”
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DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139
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Genome-wide maps of chromatin state in pluripotent and lineage-committed cells
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Regulation of chromatin structure by site-specific histone H3 methyltransferases
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“For example, trimethylation of K4 on H3 is required for the maximal activation of several genes in Saccharomyces cerevisiae (50), while trimethylation of K9 silences nearby chromatin ( 45 ).”
BackgroundActive Genes Are Tri-Methylated at K4 of Histone H3
Santos-Rosa, H., Schneider, R., Bannister, A. J., Sherriff, J., Bernstein, B. E., Emre, N. C. T., Schreiber, S. L., Mellor, J., & Kouzarides, T. (2002) · Nature · doi:10.1038/nature01080 · Cited by 2,138
Cited by
Playing the End Game: DNA Double-Strand Break Repair Pathway Choice
Chapman, J. R., Taylor, M. R. G., & Boulton, S. J. (2012) · Molecular Cell · doi:10.1016/j.molcel.2012.07.029 · Cited by 1,625
Histone post-translational modifications - cause and consequence of genome function
Millán-Zambrano, G., Burton, A., Bannister, A. J., & Schneider, R. (2022) · Nature Reviews Genetics · doi:10.1038/s41576-022-00468-7 · Cited by 1,116
Chromatin replication and epigenome maintenance
Alabert, C., & Groth, A. (2012) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3288 · Cited by 592
Acetylation limits 53BP1 association with damaged chromatin to promote homologous recombination
Tang, J., Cho, N. W., Cui, G., Manion, E. M., Shanbhag, N. M., Botuyan, M. V., Mer, G., & Greenberg, R. A. (2013) · Nature Structural & Molecular Biology · doi:10.1038/nsmb.2499 · Cited by 521
H3K36 Methylation Antagonizes PRC2-mediated H3K27 Methylation
Yuan, W., Xu, M., Huang, C., Liu, N., Chen, S., & Zhu, B. (2011) · Journal of Biological Chemistry · doi:10.1074/jbc.m110.194027 · Cited by 542
Metadata
- Authors
- Pesavento, J. J., Yang, H., Kelleher, N. L., & Mizzen, C. A.
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 2008-01-01
- Volume
- 28
- Issue
- 1
- Pages
- 468-486
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- OpenAlex
- W2156343724
- Cited by
- 289
- References
- 7
- FWCI
- 5.75
- Type
- journal article
- Language
- en
- Open access
- green
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