The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Targeted Disruption of the Basic Krüppel-Like Factor Gene (Klf3) Reveals a Role in Adipogenesis
Open accessAbstract
Krüppel-like factors (KLFs) recognize CACCC and GC-rich sequences in gene regulatory elements. Here, we describe the disruption of the murine basic Krüppel-like factor gene (Bklf or Klf3). Klf3 knockout mice have less white adipose tissue, and their fat pads contain smaller and fewer cells. Adipocyte differentiation is altered in murine embryonic fibroblasts from Klf3 knockouts. Klf3 expression was studied in the 3T3-L1 cellular system. Adipocyte differentiation is accompanied by a decline in Klf3 expression, and forced overexpression of Klf3 blocks 3T3-L1 differentiation. Klf3 represses transcription by recruiting C-terminal binding protein (CtBP) corepressors. CtBPs bind NADH and may function as metabolic sensors. A Klf3 mutant that does not bind CtBP cannot block adipogenesis. Other KLFs, Klf2, Klf5, and Klf15, also regulate adipogenesis, and functional CACCC elements occur in key adipogenic genes, including in the C/ebpalpha promoter. We find that C/ebpalpha is derepressed in Klf3 and Ctbp knockout fibroblasts and adipocytes from Klf3 knockout mice. Chromatin immunoprecipitations confirm that Klf3 binds the C/ebpalpha promoter in vivo. These results implicate Klf3 and CtBP in controlling adipogenesis.
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Cites
Adipocyte differentiation from the inside out
Rosen, E. D., & MacDougald, O. A. (2006) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2066 · Cited by 2,607
CCAAT-Enhancer Binding Protein: A Component of a Differentiation Switch
Umek, R. M., Friedman, A. D., & McKnight, S. L. (1991) · Science · doi:10.1126/science.1987644 · Cited by 712
Crossley, M., Whitelaw, E., Perkins, A., Williams, G., Fujiwara, Y., & Orkin, S. H. (1996) · Molecular and Cellular Biology · doi:10.1128/mcb.16.4.1695 · Cited by 222
Cited by
Mammalian Krüppel-Like Factors in Health and Diseases
McConnell, B. B., & Yang, V. W. (2010) · Physiological Reviews · doi:10.1152/physrev.00058.2009 · Cited by 1,018
New developments in adipogenesis
Lefterova, M. I., & Lazar, M. A. (2009) · Trends in Endocrinology & Metabolism · doi:10.1016/j.tem.2008.11.005 · Cited by 863
Lowe, C. E., O'Rahilly, S., & Rochford, J. J. (2011) · Journal of Cell Science · doi:10.1242/jcs.079699 · Cited by 345
Targeted deletion of autophagy-related 5(atg5) impairs adipogenesis in a cellular model and in mice
Baerga, R., Zhang, Y., Chen, P.-H., Goldman, S., & Jin, S. V. (2009) · Autophagy · doi:10.4161/auto.5.8.9991 · Cited by 304
Adipogenesis: A Complex Interplay of Multiple Molecular Determinants and Pathways
Ambele, M. A., Dhanraj, P., Giles, R., & Pepper, M. S. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21124283 · Cited by 300
Metadata
- Authors
- Sue, N., Jack, B. H. A., Eaton, S. A., Pearson, R. C. M., Funnell, A. P. W., Turner, J., Czolij, R., Denyer, G., Bao, S., Molero-Navajas, J. C., Perkins, A., Fujiwara, Y., Orkin, S. H., Bell-Anderson, K., & Crossley, M.
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 2008-06-01
- Volume
- 28
- Issue
- 12
- Pages
- 3967-3978
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- OpenAlex
- W2110269473
- Cited by
- 186
- References
- 3
- FWCI
- 4.34
- Type
- journal article
- Language
- en
- Open access
- green
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- Sue, N., Jack, B. H. A., Eaton, S. A., Pearson, R. C. M., Funnell, A. P. W., Turner, J., Czolij, R., Denyer, G., Bao, S., Molero-Navajas, J. C., Perkins, A., Fujiwara, Y., Orkin, S. H., Bell-Anderson, K., & Crossley, M. (2008). Targeted Disruption of the Basic Krüppel-Like Factor Gene (Klf3) Reveals a Role in Adipogenesis. Molecular and Cellular Biology. https://doi.org/10.1128/mcb.01942-07
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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